################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TseI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 787 GTCTAC GT^[AC][GT]AC AseI 1332 ATTAAT AT^TAAT AsnI 1332 ATTAAT AT^TAAT AspHI 46 GAGCTC G[AT]GC[AT]^C AvaII 1351 GGTCC G^G[AT]CC BamHI 1591 GGATCC G^GATCC BanI 102 GGCACC G^G[CT][GA]CC BanII 46 GAGCTC G[GA]GC[CT]^C BciVI 1181 GGATAC GTATCC(6/5) BclI 861 TGATCA T^GATCA BglII 930 AGATCT A^GATCT BmyI 46 GAGCTC G[AGT]GC[ACT]^C BsaAI 1229 TACGTG [CT]AC^GT[GA] BseRI 97 GAGGAG GAGGAG(10/8) BsgI 1378 GTGCAG GTGCAG(16/14) BsiHKAI 46 GAGCTC G[AT]GC[AT]^C BsmAI 393 GAGAC GTCTC(1/5) BsmFI 871 GTCCC GGGAC(10/14) BsmI 201 GCATTC GAATGC(1/-1) Bsp1286I 46 GAGCTC G[AGT]GC[ACT]^C BspHI 851 TCATGA T^CATGA BspMI 105 ACCTGC ACCTGC(4/8) BstAPI 1129 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1392 TTCGAA TT^CGAA DraI 82 TTTAAA TTT^AAA DsaI 1541 CCGTGG C^C[GA][CT]GG EarI 1116 GAAGAG CTCTTC(1/4) EcoT14I 902 CCAAGG C^C[AT][AT]GG FbaI 861 TGATCA T^GATCA HaeIII 52 GGCC GG^CC HphI 899 TCACC GGTGA(8/7) Ksp632I 1116 GAAGAG CTCTTC(1/4) MslI 438 CATTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 1129 GCATATTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 173 CATATG CA^TATG NspV 1392 TTCGAA TT^CGAA PflMI 700 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1332 ATTAAT AT^TAAT RcaI 851 TCATGA T^CATGA SacI 46 GAGCTC GAGCT^C SfaNI 154 GATGC GCATC(5/9) SfcI 723 CTATAG C^T[GA][CT]AG SfuI 1392 TTCGAA TT^CGAA SmlI 926 CTCAAG C^T[CT][GA]AG StyI 902 CCAAGG C^C[AT][AT]GG Tsp45I 262 GTCAC ^GT[GC]AC Van91I 700 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 756 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 1228 GTAC GT^AC 1303 GTAC Asp700I 1084 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1292 GAAAATGTTC BsiYI 700 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 831 CCATAATAGGG BslI 700 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 831 CCATAATAGGG BssKI 27 CCTGG ^CC[ACGT]GG 246 CCTGG BstNI 27 CCTGG CC^[AT]GG 246 CCTGG BstYI 930 AGATCT [GA]^GATC[CT] 1591 GGATCC Cfr13I 52 GGCCC G^G[ACGT]CC 1351 GGTCC EcoRII 27 CCTGG ^CC[AT]GG 246 CCTGG FauI 31 GCGGG CCCGC(4/6) 609 GCGGG MaeIII 262 GTCAC ^GT[ACGT]AC 670 GTTAC MfeI 206 CAATTG C^AATTG 1416 CAATTG MflI 930 AGATCT [GA]^GATC[CT] 1591 GGATCC MunI 206 CAATTG C^AATTG 1416 CAATTG MvaI 27 CCTGG CC^[AT]GG 246 CCTGG NlaIII 360 CATG CATG^ 852 CATG PleI 35 GAGTC GAGTC(4/5) 785 GAGTC RsaI 1228 GTAC GT^AC 1303 GTAC Sau96I 52 GGCCC G^G[ACGT]CC 1351 GGTCC ScrFI 27 CCTGG CC^[ACGT]GG 246 CCTGG SspI 502 AATATT AAT^ATT 1329 AATATT XhoII 930 AGATCT [GA]^GATC[CT] 1591 GGATCC XmnI 1084 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1292 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BfaI BsaJI BsrI BstF5I DdeI DpnII FokI HinfI MaeI MaeII MboI MboII MnlI MseI NdeII NlaIV Sau3AI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I