Restriction map for
pFN21AE5609
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TseI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 787 GTCTAC GT^[AC][GT]AC
AseI 1332 ATTAAT AT^TAAT
AsnI 1332 ATTAAT AT^TAAT
AspHI 46 GAGCTC G[AT]GC[AT]^C
AvaII 1351 GGTCC G^G[AT]CC
BamHI 1591 GGATCC G^GATCC
BanI 102 GGCACC G^G[CT][GA]CC
BanII 46 GAGCTC G[GA]GC[CT]^C
BciVI 1181 GGATAC GTATCC(6/5)
BclI 861 TGATCA T^GATCA
BglII 930 AGATCT A^GATCT
BmyI 46 GAGCTC G[AGT]GC[ACT]^C
BsaAI 1229 TACGTG [CT]AC^GT[GA]
BseRI 97 GAGGAG GAGGAG(10/8)
BsgI 1378 GTGCAG GTGCAG(16/14)
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
BsmAI 393 GAGAC GTCTC(1/5)
BsmFI 871 GTCCC GGGAC(10/14)
BsmI 201 GCATTC GAATGC(1/-1)
Bsp1286I 46 GAGCTC G[AGT]GC[ACT]^C
BspHI 851 TCATGA T^CATGA
BspMI 105 ACCTGC ACCTGC(4/8)
BstAPI 1129 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1392 TTCGAA TT^CGAA
DraI 82 TTTAAA TTT^AAA
DsaI 1541 CCGTGG C^C[GA][CT]GG
EarI 1116 GAAGAG CTCTTC(1/4)
EcoT14I 902 CCAAGG C^C[AT][AT]GG
FbaI 861 TGATCA T^GATCA
HaeIII 52 GGCC GG^CC
HphI 899 TCACC GGTGA(8/7)
Ksp632I 1116 GAAGAG CTCTTC(1/4)
MslI 438 CATTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 1129 GCATATTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 173 CATATG CA^TATG
NspV 1392 TTCGAA TT^CGAA
PflMI 700 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1332 ATTAAT AT^TAAT
RcaI 851 TCATGA T^CATGA
SacI 46 GAGCTC GAGCT^C
SfaNI 154 GATGC GCATC(5/9)
SfcI 723 CTATAG C^T[GA][CT]AG
SfuI 1392 TTCGAA TT^CGAA
SmlI 926 CTCAAG C^T[CT][GA]AG
StyI 902 CCAAGG C^C[AT][AT]GG
Tsp45I 262 GTCAC ^GT[GC]AC
Van91I 700 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 756 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1228 GTAC GT^AC
1303 GTAC
Asp700I 1084 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1292 GAAAATGTTC
BsiYI 700 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
831 CCATAATAGGG
BslI 700 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
831 CCATAATAGGG
BssKI 27 CCTGG ^CC[ACGT]GG
246 CCTGG
BstNI 27 CCTGG CC^[AT]GG
246 CCTGG
BstYI 930 AGATCT [GA]^GATC[CT]
1591 GGATCC
Cfr13I 52 GGCCC G^G[ACGT]CC
1351 GGTCC
EcoRII 27 CCTGG ^CC[AT]GG
246 CCTGG
FauI 31 GCGGG CCCGC(4/6)
609 GCGGG
MaeIII 262 GTCAC ^GT[ACGT]AC
670 GTTAC
MfeI 206 CAATTG C^AATTG
1416 CAATTG
MflI 930 AGATCT [GA]^GATC[CT]
1591 GGATCC
MunI 206 CAATTG C^AATTG
1416 CAATTG
MvaI 27 CCTGG CC^[AT]GG
246 CCTGG
NlaIII 360 CATG CATG^
852 CATG
PleI 35 GAGTC GAGTC(4/5)
785 GAGTC
RsaI 1228 GTAC GT^AC
1303 GTAC
Sau96I 52 GGCCC G^G[ACGT]CC
1351 GGTCC
ScrFI 27 CCTGG CC^[ACGT]GG
246 CCTGG
SspI 502 AATATT AAT^ATT
1329 AATATT
XhoII 930 AGATCT [GA]^GATC[CT]
1591 GGATCC
XmnI 1084 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1292 GAAAATGTTC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BfaI BsaJI BsrI BstF5I DdeI
DpnII FokI HinfI MaeI MaeII
MboI MboII MnlI MseI NdeII
NlaIV Sau3AI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I