Restriction map for
pFN21AE6227
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MseI MslI MunI NarI NciI
NcoI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tsp45I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 88 GGTACC G^GTACC
AccI 439 GTCTAC GT^[AC][GT]AC
AccII 65 CGCG CG^CG
AcyI 562 GACGCC G[GA]^CG[CT]C
AlwI 146 GGATC GGATC(4/5)
Asp718I 88 GGTACC G^GTACC
AspHI 226 GAGCTC G[AT]GC[AT]^C
AspI 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 542 TGGCCA TGG^CCA
BanI 88 GGTACC G^G[CT][GA]CC
BanII 226 GAGCTC G[GA]GC[CT]^C
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
BglI 19 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 226 GAGCTC G[AGT]GC[ACT]^C
BsaHI 562 GACGCC G[GA]^CG[CT]C
BsgI 516 CTGCAC GTGCAG(16/14)
BsiHKAI 226 GAGCTC G[AT]GC[AT]^C
BsmFI 455 GGGAC GGGAC(10/14)
Bsp1286I 226 GAGCTC G[AGT]GC[ACT]^C
BstUI 65 CGCG CG^CG
CfoI 474 GCGC GCG^C
DdeI 550 CTCAG C^T[ACGT]AG
DsaI 71 CCGTGG C^C[GA][CT]GG
EarI 127 CTCTTC CTCTTC(1/4)
EcoT14I 305 CCTTGG C^C[AT][AT]GG
HhaI 474 GCGC GCG^C
Hin1I 562 GACGCC G[GA]^CG[CT]C
HinP1I 474 GCGC G^CGC
HincII 160 GTCAAC GT[CT]^[GA]AC
HindII 160 GTCAAC GT[CT]^[GA]AC
HinfI 457 GACTC G^A[ACGT]TC
KpnI 88 GGTACC GGTAC^C
Ksp632I 127 CTCTTC CTCTTC(1/4)
MaeII 212 ACGT A^CGT
MaeIII 209 GTAAC ^GT[ACGT]AC
MluNI 542 TGGCCA TGG^CCA
MscI 542 TGGCCA TGG^CCA
MvnI 65 CGCG CG^CG
NaeI 15 GCCGGC GCC^GGC
NgoMIV 15 GCCGGC G^CCGGC
NruI 64 TCGCGA TCG^CGA
PflFI 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 521 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 457 GACTC GAGTC(4/5)
PvuII 400 CAGCTG CAG^CTG
SacI 226 GAGCTC GAGCT^C
SfaNI 396 GATGC GCATC(5/9)
SmlI 459 CTCAAG C^T[CT][GA]AG
StuI 417 AGGCCT AGG^CCT
StyI 305 CCTTGG C^C[AT][AT]GG
Tsp509I 8 AATT ^AATT
TspRI 514 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 521 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 271 TCTAGA T^CTAGA
XcmI 290 CCATGTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BfaI 250 CTAG C^TAG
272 CTAG
BglII 35 AGATCT A^GATCT
383 AGATCT
BsiYI 169 CCGCAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
521 CCATCCGCTGG
BslI 169 CCGCAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
521 CCATCCGCTGG
BsrFI 15 GCCGGC [GA]^CCGG[CT]
602 ACCGGC
BsrI 114 ACTGG ACTGG(1/-1)
143 ACTGG
BstYI 35 AGATCT [GA]^GATC[CT]
383 AGATCT
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
602 ACCGGC
HapII 16 CCGG C^CGG
603 CCGG
HgaI 562 GACGC GACGC(5/10)
158 GCGTC
HpaII 16 CCGG C^CGG
603 CCGG
HphI 3 GGTGA GGTGA(8/7)
95 TCACC
MaeI 250 CTAG C^TAG
272 CTAG
MboII 128 TCTTC GAAGA(8/7)
353 TCTTC
MflI 35 AGATCT [GA]^GATC[CT]
383 AGATCT
MspA1I 400 CAGCTG C[AC]G^C[GT]G
525 CCGCTG
MspI 16 CCGG C^CGG
603 CCGG
NspI 311 GCATGT [GA]CATG^[CT]
476 GCATGT
XhoII 35 AGATCT [GA]^GATC[CT]
383 AGATCT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BseRI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EaeI EcoRII Fnu4HI
FokI HaeIII ItaI MboI MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI