Restriction map for
pFN21AE6108
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboII MfeI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 157 GGCGCC G[GA]^CG[CT]C
AlwI 342 GGATC GGATC(4/5)
AvaI 109 CCCGGG C^[CT]CG[GA]G
AvaII 151 GGACC G^G[AT]CC
BanI 157 GGCGCC G^G[CT][GA]CC
BbeI 157 GGCGCC GGCGC^C
BglI 33 GCCGTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 165 GGGCAC G[AGT]GC[ACT]^C
BpmI 184 CTGGAG CTGGAG(16/14)
BsaHI 157 GGCGCC G[GA]^CG[CT]C
BsiEI 70 CGGCCG CG[GA][CT]^CG
BsiYI 73 CCGCCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 73 CCGCCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 109 CCCGGG C^[CT]CG[GA]G
Bsp1286I 165 GGGCAC G[AGT]GC[ACT]^C
BsrDI 358 CATTGC GCAATG(2/0)
BsrFI 68 GCCGGC [GA]^CCGG[CT]
BssHII 91 GCGCGC G^CGCGC
BstF5I 48 CATCC GGATG(2/0)
BstYI 342 GGATCT [GA]^GATC[CT]
Cfr10I 68 GCCGGC [GA]^CCGG[CT]
CpoI 150 CGGACCG CG^G[AT]CCG
DpnII 343 GATC ^GATC
DraII 271 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 70 CGGCCG C^GGCCG
EclXI 70 CGGCCG C^GGCCG
Eco52I 70 CGGCCG C^GGCCG
EcoO109I 271 AGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 48 CATCC GGATG(9/13)
HaeII 157 GGCGCC [GA]GCGC^[CT]
Hin1I 157 GGCGCC G[GA]^CG[CT]C
HinfI 244 GAATC G^A[ACGT]TC
KasI 157 GGCGCC G^GCGCC
MboI 343 GATC ^GATC
MflI 342 GGATCT [GA]^GATC[CT]
MseI 278 TTAA T^TAA
MspA1I 147 CAGCGG C[AC]G^C[GT]G
NaeI 68 GCCGGC GCC^GGC
NarI 157 GGCGCC GG^CGCC
NdeII 343 GATC ^GATC
NgoMIV 68 GCCGGC G^CCGGC
NlaIII 96 CATG CATG^
NspI 95 GCATGC [GA]CATG^[CT]
PstI 239 CTGCAG CTGCA^G
RsrII 150 CGGACCG CG^G[AT]CCG
Sau3AI 343 GATC ^GATC
SfaNI 47 GCATC GCATC(5/9)
SfcI 239 CTGCAG C^T[GA][CT]AG
SfoI 157 GGCGCC GGC^GCC
SmaI 109 CCCGGG CCC^GGG
SphI 95 GCATGC GCATG^C
TfiI 244 GAATC G^A[AT]TC
Tru9I 278 TTAA T^TAA
XhoII 342 GGATCT [GA]^GATC[CT]
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 87 GTAC GT^AC
326 GTAC
AluI 188 AGCT AG^CT
253 AGCT
BsrI 288 ACTGG ACTGG(1/-1)
338 ACTGG
BstNI 161 CCTGG CC^[AT]GG
183 CCTGG
DdeI 268 CTGAG C^T[ACGT]AG
293 CTCAG
EaeI 70 CGGCCG [CT]^GGCC[GA]
143 CGGCCA
EcoRII 161 CCTGG ^CC[AT]GG
183 CCTGG
MvaI 161 CCTGG CC^[AT]GG
183 CCTGG
NlaIV 30 GGGGCC GG[ACGT]^[ACGT]CC
157 GGCGCC
RsaI 87 GTAC GT^AC
326 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI BbvI BcnI BsaJI
BssKI BstUI Cac8I CfoI Cfr13I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvnI
MwoI NciI Sau96I ScrFI TseI
TspRI