Restriction map for
pFN21AE4553
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FspI HincII HindII
HindIII HpaI KpnI MfeI MluNI
MroI MscI MslI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AclI 773 AACGTT AA^CGTT
AflII 1453 CTTAAG C^TTAAG
AflIII 1905 ACGCGT A^C[GA][CT]GT
AspI 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 592 CGAGAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1016 GTATCC GTATCC(6/5)
BclI 1103 TGATCA T^GATCA
BfrI 1453 CTTAAG C^TTAAG
BglI 1492 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1641 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 322 GTGCAG GTGCAG(16/14)
BsmBI 1044 GAGACG CGTCTC(1/5)
BsmI 357 GAATGC GAATGC(1/-1)
BspMI 313 GCAGGT ACCTGC(4/8)
BsrDI 1032 CATTGC GCAATG(2/0)
BssSI 947 CTCGTG CACGAG(-5/-1)
FbaI 1103 TGATCA T^GATCA
FseI 493 GGCCGGCC GGCCGG^CC
MaeII 774 ACGT A^CGT
MamI 1641 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1905 ACGCGT A^CGCGT
PflFI 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 773 AACGTT AA^CGTT
PvuII 210 CAGCTG CAG^CTG
SacI 1867 GAGCTC GAGCT^C
ScaI 292 AGTACT AGT^ACT
SexAI 581 ACCTGGT A^CC[AT]GGT
SmlI 1453 CTTAAG C^T[CT][GA]AG
Tth111I 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 219 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1763 GGCGCC G[GA]^CG[CT]C
1987 GGCGCC
Aor51HI 722 AGCGCT AGC^GCT
1979 AGCGCT
ApaI 503 GGGCCC GGGCC^C
1370 GGGCCC
AspHI 745 GTGCTC G[AT]GC[AT]^C
1867 GAGCTC
AvaI 1205 CCCGAG C^[CT]CG[GA]G
1802 CCCGAG
BbeI 1763 GGCGCC GGCGC^C
1987 GGCGCC
BglII 533 AGATCT A^GATCT
1806 AGATCT
BsaHI 1763 GGCGCC G[GA]^CG[CT]C
1987 GGCGCC
BsiHKAI 745 GTGCTC G[AT]GC[AT]^C
1867 GAGCTC
BsoBI 1205 CCCGAG C^[CT]CG[GA]G
1802 CCCGAG
BsrBI 664 GAGCGG CCGCTC(-3/-3)
1399 GAGCGG
BstAPI 402 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
669 GCACTTCCTGC
BstXI 1481 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1977 CCAGCGCTGTGG
Eco47III 722 AGCGCT AGC^GCT
1979 AGCGCT
Hin1I 1763 GGCGCC G[GA]^CG[CT]C
1987 GGCGCC
KasI 1763 GGCGCC G^GCGCC
1987 GGCGCC
KspI 5 CCGCGG CCGC^GG
442 CCGCGG
MaeIII 514 GTCAC ^GT[ACGT]AC
1558 GTCAC
MseI 1157 TTAA T^TAA
1454 TTAA
NaeI 494 GCCGGC GCC^GGC
1972 GCCGGC
NarI 1763 GGCGCC GG^CGCC
1987 GGCGCC
NcoI 499 CCATGG C^CATGG
1244 CCATGG
NgoMIV 494 GCCGGC G^CCGGC
1972 GCCGGC
PstI 463 CTGCAG CTGCA^G
1132 CTGCAG
SacII 5 CCGCGG CCGC^GG
442 CCGCGG
SfoI 1763 GGCGCC GGC^GCC
1987 GGCGCC
TaqI 16 TCGA T^CGA
687 TCGA
TfiI 618 GATTC G^A[AT]TC
933 GAATC
Tru9I 1157 TTAA T^TAA
1454 TTAA
Tsp45I 514 GTCAC ^GT[GC]AC
1558 GTCAC
Tsp509I 1175 AATT ^AATT
1608 AATT
TspRI 320 CAGTG CA[GC]TG[ACGT][ACGT]^
363 CAGTG
TthHB8I 16 TCGA T^CGA
687 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
XhoII