Restriction map for
pFN21AE1425
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccI 316 GTAGAC GT^[AC][GT]AC
AcsI 57 AAATTT [GA]^AATT[CT]
AcyI 319 GACGCC G[GA]^CG[CT]C
ApoI 57 AAATTT [GA]^AATT[CT]
AspHI 586 GTGCTC G[AT]GC[AT]^C
BalI 218 TGGCCA TGG^CCA
BbsI 846 GAAGAC GAAGAC(2/6)
BpuAI 846 GAAGAC GAAGAC(2/6)
BsaHI 319 GACGCC G[GA]^CG[CT]C
BsaI 754 GAGACC GGTCTC(1/5)
BsiHKAI 586 GTGCTC G[AT]GC[AT]^C
BsmFI 206 GGGAC GGGAC(10/14)
BspMI 484 ACCTGC ACCTGC(4/8)
BssSI 112 CTCGTG CACGAG(-5/-1)
Cac8I 810 GCTCGC GC[ACGT]^[ACGT]GC
EcoNI 901 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 319 GACGCC G[GA]^CG[CT]C
MaeII 689 ACGT A^CGT
MluNI 218 TGGCCA TGG^CCA
MscI 218 TGGCCA TGG^CCA
NspI 408 GCATGT [GA]CATG^[CT]
PpuMI 1084 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 542 CAGCTG CAG^CTG
SfaNI 311 GCATC GCATC(5/9)
SmlI 966 CTCAAG C^T[CT][GA]AG
Tsp509I 58 AATT ^AATT
XbaI 456 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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ApaI 38 GGGCCC GGGCC^C
561 GGGCCC
AvaII 612 GGACC G^G[AT]CC
1085 GGACC
BamHI 51 GGATCC G^GATCC
282 GGATCC
BanI 619 GGCACC G^G[CT][GA]CC
979 GGTGCC
BanII 38 GGGCCC G[GA]GC[CT]^C
561 GGGCCC
BfaI 232 CTAG C^TAG
457 CTAG
BpmI 212 CTCCAG CTGGAG(16/14)
650 CTGGAG
BsgI 120 CTGCAC GTGCAG(16/14)
73 GTGCAG
BstXI 55 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCACCTCCCTGG
BstYI 51 GGATCC [GA]^GATC[CT]
282 GGATCC
DsaI 601 CCACGG C^C[GA][CT]GG
615 CCACGG
EaeI 218 TGGCCA [CT]^GGCC[GA]
465 CGGCCA
EcoT14I 276 CCTTGG C^C[AT][AT]GG
1052 CCAAGG
HgaI 319 GACGC GACGC(5/10)
107 GCGTC
MaeI 232 CTAG C^TAG
457 CTAG
MflI 51 GGATCC [GA]^GATC[CT]
282 GGATCC
MspA1I 4 CCGCTG C[AC]G^C[GT]G
542 CAGCTG
StyI 276 CCTTGG C^C[AT][AT]GG
1052 CCAAGG
TfiI 266 GATTC G^A[AT]TC
1044 GAATC
XcmI 758 CCAGAGCAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
869 CCAGGACAGCAGTGG
XhoII 51 GGATCC [GA]^GATC[CT]
282 GGATCC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
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AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI Van91I