Restriction map for
pFN21AE1490
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BbeI BbrPI
BciVI BclI BfaI BfrI BlnI
BsaHI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI EcoNI EcoO65I EcoRV
EcoT22I FbaI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I MaeI
MfeI MluI MluNI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1963 GTCGAC GT^[AC][GT]AC
AcsI 937 GAATTC [GA]^AATT[CT]
ApaI 1384 GGGCCC GGGCC^C
ApoI 937 GAATTC [GA]^AATT[CT]
AviII 648 TGCGCA TGC^GCA
BanI 1208 GGCACC G^G[CT][GA]CC
BbsI 1486 GAAGAC GAAGAC(2/6)
BbvCI 1695 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1486 GAAGAC GAAGAC(2/6)
BsaAI 1912 TACGTG [CT]AC^GT[GA]
BsaBI 1773 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1410 GAGACC GGTCTC(1/5)
BsgI 194 GTGCAG GTGCAG(16/14)
BsmBI 1942 GAGACG CGTCTC(1/5)
BsmI 559 GCATTC GAATGC(1/-1)
BsrDI 1868 GCAATG GCAATG(2/0)
BsrI 363 ACTGG ACTGG(1/-1)
BstXI 1340 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 937 GAATTC G^AATTC
FseI 929 GGCCGGCC GGCCGG^CC
FspI 648 TGCGCA TGC^GCA
HincII 1963 GTCGAC GT[CT]^[GA]AC
HindII 1963 GTCGAC GT[CT]^[GA]AC
KspI 925 CCGCGG CCGC^GG
MamI 1773 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1767 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SacI 1170 GAGCTC GAGCT^C
SacII 925 CCGCGG CCGC^GG
SalI 1963 GTCGAC G^TCGAC
SmaI 1380 CCCGGG CCC^GGG
SmlI 1096 CTCAAG C^T[CT][GA]AG
Tsp509I 938 AATT ^AATT
XmaCI 1380 CCCGGG C^CCGGG
XmaI 1380 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccIII 44 TCCGGA T^CCGGA
1727 TCCGGA
AflIII 665 ACGTGT A^C[GA][CT]GT
1798 ACACGT
Asp700I 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1731 GAAGAAGTTC
AspHI 160 GAGCAC G[AT]GC[AT]^C
1170 GAGCTC
BamHI 1644 GGATCC G^GATCC
1826 GGATCC
BcgI 1398 GCAGAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1814 GCAAGCACCTCG
BglII 1431 AGATCT A^GATCT
1511 AGATCT
BlpI 153 GCTAAGC GC^T[ACGT]AGC
1215 GCTCAGC
Bpu1102I 153 GCTAAGC GC^T[ACGT]AGC
1215 GCTCAGC
BsaWI 44 TCCGGA [AT]^CCGG[AT]
1727 TCCGGA
BseAI 44 TCCGGA T^CCGGA
1727 TCCGGA
BsiHKAI 160 GAGCAC G[AT]GC[AT]^C
1170 GAGCTC
BspEI 44 TCCGGA T^CCGGA
1727 TCCGGA
CelII 153 GCTAAGC GC^T[ACGT]AGC
1215 GCTCAGC
MroI 44 TCCGGA T^CCGGA
1727 TCCGGA
NcoI 23 CCATGG C^CATGG
1670 CCATGG
NlaIII 24 CATG CATG^
1671 CATG
PvuII 397 CAGCTG CAG^CTG
1693 CAGCTG
XmnI 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1731 GAAGAAGTTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI Aor51HI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII