Restriction map for
pFN21AE0955
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AlwI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I CelII CpoI DraI DraIII
EaeI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SphI Sse8387I SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1413 GTCGAC GT^[AC][GT]AC
AcyI 175 GGCGTC G[GA]^CG[CT]C
AflIII 671 ACATGT A^C[GA][CT]GT
AhdI 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 742 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 335 AGCGCT AGC^GCT
ApaI 857 GGGCCC GGGCC^C
AspEI 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1194 TGCGCA TGC^GCA
BanI 133 GGCACC G^G[CT][GA]CC
BsaHI 175 GGCGTC G[GA]^CG[CT]C
BsaI 418 GAGACC GGTCTC(1/5)
BsiEI 1415 CGACCG CG[GA][CT]^CG
BsmBI 1301 CGTCTC CGTCTC(1/5)
Bsp1407I 668 TGTACA T^GTACA
BspDI 901 ATCGAT AT^CGAT
BspLU11I 671 ACATGT A^CATGT
BspMI 746 ACCTGC ACCTGC(4/8)
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 377 GCAATG GCAATG(2/0)
BsrFI 136 ACCGGC [GA]^CCGG[CT]
BsrGI 668 TGTACA T^GTACA
BstAPI 483 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 297 GGTGACC G^GT[ACGT]ACC
BstPI 297 GGTGACC G^GT[ACGT]ACC
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
ClaI 901 ATCGAT AT^CGAT
DrdI 763 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 164 CCATGG C^C[GA][CT]GG
Eam1105I 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 335 AGCGCT AGC^GCT
EcoNI 16 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 297 GGTGACC G^GT[ACGT]ACC
FspI 1194 TGCGCA TGC^GCA
HaeII 335 AGCGCT [GA]GCGC^[CT]
HgaI 176 GCGTC GACGC(5/10)
Hin1I 175 GGCGTC G[GA]^CG[CT]C
HindIII 1376 AAGCTT A^AGCTT
HinfI 1389 GACTC G^A[ACGT]TC
NcoI 164 CCATGG C^CATGG
NspI 671 ACATGT [GA]CATG^[CT]
PaeR7I 186 CTCGAG C^TCGAG
PleI 1389 GACTC GAGTC(4/5)
PpuMI 1336 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 300 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 481 CTGCAG CTGCA^G
SalI 1413 GTCGAC G^TCGAC
SapI 681 GCTCTTC GCTCTTC(1/4)
SfcI 481 CTGCAG C^T[GA][CT]AG
SgrAI 135 CACCGGCG C[GA]^CCGG[CT]G
SpeI 429 ACTAGT A^CTAGT
SspBI 668 TGTACA T^GTACA
SspI 511 AATATT AAT^ATT
StuI 1187 AGGCCT AGG^CCT
XhoI 186 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BsmFI 965 GGGAC GGGAC(10/14)
1338 GTCCC
DpnII 486 GATC ^GATC
993 GATC
EarI 682 CTCTTC CTCTTC(1/4)
1264 CTCTTC
HincII 639 GTTGAC GT[CT]^[GA]AC
1413 GTCGAC
HindII 639 GTTGAC GT[CT]^[GA]AC
1413 GTCGAC
Ksp632I 682 CTCTTC CTCTTC(1/4)
1264 CTCTTC
MboI 486 GATC ^GATC
993 GATC
MseI 1076 TTAA T^TAA
1381 TTAA
MslI 363 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATCAAGGTG
NdeII 486 GATC ^GATC
993 GATC
PflMI 342 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
981 CCAGCTGGTGG
Sau3AI 486 GATC ^GATC
993 GATC
SfaNI 1420 GATGC GCATC(5/9)
151 GCATC
SmaI 265 CCCGGG CCC^GGG
1019 CCCGGG
Tru9I 1076 TTAA T^TAA
1381 TTAA
Van91I 342 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
981 CCAGCTGGTGG
XmaCI 265 CCCGGG C^CCGGG
1019 CCCGGG
XmaI 265 CCCGGG C^CCGGG
1019 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeI MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PvuII RsaI SacI
Sau96I ScrFI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I