Restriction map for
pFN21AE3659
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 433 GTAC GT^AC
AluI 189 AGCT AG^CT
Aor51HI 69 AGCGCT AGC^GCT
AspHI 301 GAGCAC G[AT]GC[AT]^C
AvaI 50 CCCGAG C^[CT]CG[GA]G
AvaII 47 GGACC G^G[AT]CC
AvrII 43 CCTAGG C^CTAGG
BalI 440 TGGCCA TGG^CCA
BamHI 228 GGATCC G^GATCC
BclI 446 TGATCA T^GATCA
BcnI 12 CCGGG CC^[GC]GG
BfaI 44 CTAG C^TAG
BlnI 43 CCTAGG C^CTAGG
BlpI 124 GCTCAGC GC^T[ACGT]AGC
BmyI 301 GAGCAC G[AGT]GC[ACT]^C
BpmI 298 CTGGAG CTGGAG(16/14)
Bpu1102I 124 GCTCAGC GC^T[ACGT]AGC
BsgI 400 GTGCAG GTGCAG(16/14)
BsiEI 61 CGGCCG CG[GA][CT]^CG
BsiHKAI 301 GAGCAC G[AT]GC[AT]^C
BsmBI 37 CGTCTC CGTCTC(1/5)
BsmFI 166 GGGAC GGGAC(10/14)
BsoBI 50 CCCGAG C^[CT]CG[GA]G
Bsp1286I 301 GAGCAC G[AGT]GC[ACT]^C
BsrI 254 ACTGG ACTGG(1/-1)
BssHII 21 GCGCGC G^CGCGC
BssSI 112 CACGAG CACGAG(-5/-1)
BstXI 335 CCAATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 124 GCTCAGC GC^T[ACGT]AGC
DdeI 125 CTCAG C^T[ACGT]AG
EagI 61 CGGCCG C^GGCCG
EarI 131 CTCTTC CTCTTC(1/4)
EclXI 61 CGGCCG C^GGCCG
Eco47III 69 AGCGCT AGC^GCT
Eco52I 61 CGGCCG C^GGCCG
FauI 33 CCCGC CCCGC(4/6)
FbaI 446 TGATCA T^GATCA
HgaI 36 GCGTC GACGC(5/10)
Ksp632I 131 CTCTTC CTCTTC(1/4)
MaeI 44 CTAG C^TAG
MluNI 440 TGGCCA TGG^CCA
MscI 440 TGGCCA TGG^CCA
MspA1I 188 CAGCTG C[AC]G^C[GT]G
NciI 12 CCGGG CC^[GC]GG
PpuMI 46 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 188 CAGCTG CAG^CTG
RsaI 433 GTAC GT^AC
SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G
StuI 271 AGGCCT AGG^CCT
TaqI 236 TCGA T^CGA
Tsp509I 197 AATT ^AATT
TspRI 510 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 236 TCGA T^CGA
XcmI 386 CCAACTGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 22 CGCG CG^CG
35 CGCG
BbvI 67 GCAGC GCAGC(8/12)
402 GCAGC
BsrFI 25 GCCGGC [GA]^CCGG[CT]
63 GCCGGC
BstF5I 468 CATCC GGATG(2/0)
429 GGATG
BstUI 22 CGCG CG^CG
35 CGCG
BstYI 228 GGATCC [GA]^GATC[CT]
374 AGATCC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
63 GCCGGC
DraII 46 AGGACCC [GA]G^G[ACGT]CC[CT]
116 AGGCCCT
EaeI 61 CGGCCG [CT]^GGCC[GA]
440 TGGCCA
EcoO109I 46 AGGACCC [GA]G^G[ACGT]CC[CT]
116 AGGCCCT
EcoT14I 43 CCTAGG C^C[AT][AT]GG
341 CCTTGG
FokI 468 CATCC GGATG(9/13)
429 GGATG
HaeII 69 AGCGCT [GA]GCGC^[CT]
94 GGCGCT
HphI 171 GGTGA GGTGA(8/7)
369 TCACC
MboII 174 GAAGA GAAGA(8/7)
132 TCTTC
MflI 228 GGATCC [GA]^GATC[CT]
374 AGATCC
MvnI 22 CGCG CG^CG
35 CGCG
MwoI 23 GCGCCGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
63 GCCGGCAGCGC
NaeI 25 GCCGGC GCC^GGC
63 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
63 GCCGGC
NlaIII 261 CATG CATG^
444 CATG
NlaIV 47 GGACCC GG[ACGT]^[ACGT]CC
228 GGATCC
PleI 366 GACTC GAGTC(4/5)
53 GAGTC
PshAI 48 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
186 GACAGCTGTC
StyI 43 CCTAGG C^C[AT][AT]GG
341 CCTTGG
TfiI 105 GAATC G^A[AT]TC
207 GAATC
TseI 67 GCAGC G^C[AT]GC
402 GCAGC
XhoII 228 GGATCC [GA]^GATC[CT]
374 AGATCC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AlwI BsaJI BseRI BsiYI
BslI BsmAI BssKI BstNI Cac8I
CfoI Cfr13I DpnII EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MnlI MspI
MvaI NdeII Sau3AI Sau96I ScrFI