Restriction map for
pFN21AE1560
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 682 GTCTAC GT^[AC][GT]AC
AgeI 586 ACCGGT A^CCGGT
AlwNI 115 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 456 GAGCAC G[AT]GC[AT]^C
BamHI 567 GGATCC G^GATCC
BbsI 576 GAAGAC GAAGAC(2/6)
BcnI 605 CCGGG CC^[GC]GG
BglI 375 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 576 GAAGAC GAAGAC(2/6)
BsaI 693 GGTCTC GGTCTC(1/5)
BsaWI 586 ACCGGT [AT]^CCGG[AT]
BsiHKAI 456 GAGCAC G[AT]GC[AT]^C
BsmBI 520 CGTCTC CGTCTC(1/5)
BsrBI 247 GAGCGG CCGCTC(-3/-3)
BsrFI 586 ACCGGT [GA]^CCGG[CT]
BsrI 510 CCAGT ACTGG(1/-1)
BssSI 616 CTCGTG CACGAG(-5/-1)
BstYI 567 GGATCC [GA]^GATC[CT]
Cac8I 433 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 732 GCGC GCG^C
Cfr10I 586 ACCGGT [GA]^CCGG[CT]
DsaI 528 CCATGG C^C[GA][CT]GG
FauI 92 CCCGC CCCGC(4/6)
HaeII 731 AGCGCC [GA]GCGC^[CT]
HhaI 732 GCGC GCG^C
HinP1I 732 GCGC G^CGC
MaeIII 214 GTCAC ^GT[ACGT]AC
MflI 567 GGATCC [GA]^GATC[CT]
NciI 605 CCGGG CC^[GC]GG
NcoI 528 CCATGG C^CATGG
PinAI 586 ACCGGT A^CCGGT
SfaNI 152 GCATC GCATC(5/9)
Tsp45I 214 GTCAC ^GT[GC]AC
Tsp509I 535 AATT ^AATT
XhoII 567 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 568 GATCC GGATC(4/5)
567 GGATC
ApaI 224 GGGCCC GGGCC^C
610 GGGCCC
AvaII 251 GGACC G^G[AT]CC
359 GGACC
BanII 224 GGGCCC G[GA]GC[CT]^C
610 GGGCCC
BfaI 157 CTAG C^TAG
389 CTAG
BpmI 674 CTCCAG CTGGAG(16/14)
340 CTGGAG
BseRI 13 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsgI 423 GTGCAG GTGCAG(16/14)
496 GTGCAG
DpnII 60 GATC ^GATC
568 GATC
HapII 587 CCGG C^CGG
605 CCGG
HpaII 587 CCGG C^CGG
605 CCGG
HphI 405 GGTGA GGTGA(8/7)
215 TCACC
MaeI 157 CTAG C^TAG
389 CTAG
MaeII 519 ACGT A^CGT
629 ACGT
MboI 60 GATC ^GATC
568 GATC
MslI 153 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
363 CACAGCTGTG
MspI 587 CCGG C^CGG
605 CCGG
NdeII 60 GATC ^GATC
568 GATC
PstI 334 CTGCAG CTGCA^G
475 CTGCAG
Sau3AI 60 GATC ^GATC
568 GATC
SfcI 334 CTGCAG C^T[GA][CT]AG
475 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MboII MnlI MspA1I MvaI
MwoI NlaIII NlaIV PleI PvuII
Sau96I ScrFI StyI TseI TspRI