Restriction map for
pFN21AE4542
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BssSI BstBI BstEII BstPI BstUI
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 1083 AAATTT [GA]^AATT[CT]
Aor51HI 117 AGCGCT AGC^GCT
ApoI 1083 AAATTT [GA]^AATT[CT]
AvaI 1694 CCCGGG C^[CT]CG[GA]G
BbsI 456 GAAGAC GAAGAC(2/6)
BbvCI 1805 CCTCAGC CCTCAGC(-5/-2)
BglI 465 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 456 GAAGAC GAAGAC(2/6)
BsaAI 730 TACGTA [CT]AC^GT[GA]
BsgI 784 GTGCAG GTGCAG(16/14)
BsmBI 366 CGTCTC CGTCTC(1/5)
BsoBI 1694 CCCGGG C^[CT]CG[GA]G
Bsp1407I 176 TGTACA T^GTACA
BsrBI 1737 CCGCTC CCGCTC(-3/-3)
BsrDI 446 GCAATG GCAATG(2/0)
BsrFI 377 ACCGGC [GA]^CCGG[CT]
BsrGI 176 TGTACA T^GTACA
Bst1107I 600 GTATAC GTA^TAC
BstZ17I 600 GTATAC GTA^TAC
CfoI 118 GCGC GCG^C
Cfr10I 377 ACCGGC [GA]^CCGG[CT]
Eco47III 117 AGCGCT AGC^GCT
HaeII 117 AGCGCT [GA]GCGC^[CT]
HhaI 118 GCGC GCG^C
HinP1I 118 GCGC G^CGC
MseI 1357 TTAA T^TAA
NspI 1590 GCATGT [GA]CATG^[CT]
PshAI 927 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1707 GCTCTTC GCTCTTC(1/4)
SmaI 1694 CCCGGG CCC^GGG
SnaBI 730 TACGTA TAC^GTA
SspBI 176 TGTACA T^GTACA
Tru9I 1357 TTAA T^TAA
XbaI 999 TCTAGA T^CTAGA
XmaCI 1694 CCCGGG C^CCGGG
XmaI 1694 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 364 GACGTC GACGT^C
751 GACGTC
AlwI 684 GATCC GGATC(4/5)
294 GGATC
BanI 400 GGTGCC G^G[CT][GA]CC
836 GGTGCC
BclI 1334 TGATCA T^GATCA
1416 TGATCA
BlpI 1575 GCTGAGC GC^T[ACGT]AGC
1839 GCTGAGC
Bpu1102I 1575 GCTGAGC GC^T[ACGT]AGC
1839 GCTGAGC
BsaI 63 GGTCTC GGTCTC(1/5)
1531 GGTCTC
BspMI 131 ACCTGC ACCTGC(4/8)
1835 ACCTGC
BsrI 277 CCAGT ACTGG(1/-1)
779 CCAGT
BstAPI 315 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
336 GCAGACACTGC
BstYI 294 GGATCT [GA]^GATC[CT]
683 AGATCC
CelII 1575 GCTGAGC GC^T[ACGT]AGC
1839 GCTGAGC
DsaI 654 CCACGG C^C[GA][CT]GG
1434 CCGTGG
FbaI 1334 TGATCA T^GATCA
1416 TGATCA
HgaI 270 GACGC GACGC(5/10)
480 GACGC
MflI 294 GGATCT [GA]^GATC[CT]
683 AGATCC
PleI 1612 GACTC GAGTC(4/5)
1815 GAGTC
PstI 706 CTGCAG CTGCA^G
1666 CTGCAG
PvuII 223 CAGCTG CAG^CTG
310 CAGCTG
SfaNI 201 GATGC GCATC(5/9)
1026 GCATC
SfcI 706 CTGCAG C^T[GA][CT]AG
1666 CTGCAG
Tsp509I 796 AATT ^AATT
1084 AATT
XcmI 485 CCACACCCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
920 CCACAAGGACTGTGG
XhoII 294 GGATCT [GA]^GATC[CT]
683 AGATCC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
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AccI AciI AcyI AfaI AluI
AlwNI AspHI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI SacI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I