Restriction map for
pFN21AE1469
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BlnI BpmI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MspI MunI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 142 CGCG CG^CG
AcyI 82 GGCGCC G[GA]^CG[CT]C
AluI 269 AGCT AG^CT
AlwI 232 GATCC GGATC(4/5)
AlwNI 99 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 150 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 180 CCCGAG C^[CT]CG[GA]G
AvaII 164 GGACC G^G[AT]CC
BanI 82 GGCGCC G^G[CT][GA]CC
BbeI 82 GGCGCC GGCGC^C
BbsI 94 GAAGAC GAAGAC(2/6)
BbvI 107 GCTGC GCAGC(8/12)
BglI 74 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 221 AGATCT A^GATCT
BlpI 270 GCTGAGC GC^T[ACGT]AGC
BmyI 241 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 270 GCTGAGC GC^T[ACGT]AGC
BpuAI 94 GAAGAC GAAGAC(2/6)
BsaHI 82 GGCGCC G[GA]^CG[CT]C
BsgI 17 CTGCAC GTGCAG(16/14)
BsoBI 180 CCCGAG C^[CT]CG[GA]G
Bsp1286I 241 GTGCCC G[AGT]GC[ACT]^C
BsrI 161 ACTGG ACTGG(1/-1)
BstUI 142 CGCG CG^CG
BstYI 221 AGATCT [GA]^GATC[CT]
CelII 270 GCTGAGC GC^T[ACGT]AGC
DraII 72 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 176 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 72 AGGCCCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 107 GCTGC GC^[ACGT]GC
HgaI 101 GCGTC GACGC(5/10)
Hin1I 82 GGCGCC G[GA]^CG[CT]C
ItaI 107 GCTGC GC^[ACGT]GC
KasI 82 GGCGCC G^GCGCC
MaeII 119 ACGT A^CGT
MaeIII 125 GTTAC ^GT[ACGT]AC
MflI 221 AGATCT [GA]^GATC[CT]
MvnI 142 CGCG CG^CG
NarI 82 GGCGCC GG^CGCC
NlaIII 90 CATG CATG^
PflFI 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 172 GACTC GAGTC(4/5)
SfcI 10 CTACAG C^T[GA][CT]AG
SfoI 82 GGCGCC GGC^GCC
StuI 184 AGGCCT AGG^CCT
TfiI 258 GATTC G^A[AT]TC
TseI 107 GCTGC G^C[AT]GC
Tth111I 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 221 AGATCT [GA]^GATC[CT]
XmnI 150 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Cac8I 4 GCTCGC GC[ACGT]^[ACGT]GC
70 GCAGGC
Cfr13I 73 GGCCC G^G[ACGT]CC
164 GGACC
DpnII 222 GATC ^GATC
232 GATC
HaeII 51 GGCGCT [GA]GCGC^[CT]
82 GGCGCC
HaeIII 73 GGCC GG^CC
185 GGCC
HinfI 172 GACTC G^A[ACGT]TC
258 GATTC
HphI 147 GGTGA GGTGA(8/7)
249 GGTGA
MboI 222 GATC ^GATC
232 GATC
MboII 94 GAAGA GAAGA(8/7)
252 GAAGA
NdeII 222 GATC ^GATC
232 GATC
NlaIV 73 GGCCCC GG[ACGT]^[ACGT]CC
82 GGCGCC
Sau3AI 222 GATC ^GATC
232 GATC
Sau96I 73 GGCCC G^G[ACGT]CC
164 GGACC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BsaJI BsiYI BslI BssKI BstNI
CfoI DdeI EcoRII HhaI HinP1I
MnlI MvaI MwoI ScrFI