Restriction map for
pFN21AE1559
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HinfI
HpaI KasI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 547 GGTACC G^GTACC
AccI 148 GTCTAC GT^[AC][GT]AC
AflII 267 CTTAAG C^TTAAG
Asp718I 547 GGTACC G^GTACC
AvaI 1010 CCCGGG C^[CT]CG[GA]G
BalI 26 TGGCCA TGG^CCA
BamHI 1104 GGATCC G^GATCC
BbrPI 1342 CACGTG CAC^GTG
BbsI 408 GAAGAC GAAGAC(2/6)
BfaI 1213 CTAG C^TAG
BfrI 267 CTTAAG C^TTAAG
BlpI 75 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC
BpuAI 408 GAAGAC GAAGAC(2/6)
BsaAI 1342 CACGTG [CT]AC^GT[GA]
BsaI 11 GGTCTC GGTCTC(1/5)
BsgI 820 GTGCAG GTGCAG(16/14)
BsoBI 1010 CCCGGG C^[CT]CG[GA]G
BsrDI 745 CATTGC GCAATG(2/0)
BsrFI 511 GCCGGC [GA]^CCGG[CT]
BstEII 997 GGTGACC G^GT[ACGT]ACC
BstPI 997 GGTGACC G^GT[ACGT]ACC
CelII 75 GCTCAGC GC^T[ACGT]AGC
Cfr10I 511 GCCGGC [GA]^CCGG[CT]
EcoO65I 997 GGTGACC G^GT[ACGT]ACC
HaeII 1241 AGCGCC [GA]GCGC^[CT]
HincII 970 GTCAAC GT[CT]^[GA]AC
HindII 970 GTCAAC GT[CT]^[GA]AC
KpnI 547 GGTACC GGTAC^C
MaeI 1213 CTAG C^TAG
MaeII 1343 ACGT A^CGT
MluNI 26 TGGCCA TGG^CCA
MscI 26 TGGCCA TGG^CCA
MseI 268 TTAA T^TAA
NaeI 511 GCCGGC GCC^GGC
NcoI 5 CCATGG C^CATGG
NgoMIV 511 GCCGGC G^CCGGC
PmaCI 1342 CACGTG CAC^GTG
PmlI 1342 CACGTG CAC^GTG
SmaI 1010 CCCGGG CCC^GGG
StuI 1119 AGGCCT AGG^CCT
Tru9I 268 TTAA T^TAA
XcmI 470 CCAGGCTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1010 CCCGGG C^CCGGG
XmaI 1010 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1068 AAATTC [GA]^AATT[CT]
1222 AAATTC
AflIII 368 ACATGT A^C[GA][CT]GT
1277 ACATGT
ApoI 1068 AAATTC [GA]^AATT[CT]
1222 AAATTC
Asp700I 1218 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1405 GAAGGGGTTC
AvaII 216 GGACC G^G[AT]CC
582 GGACC
BsmAI 643 GAGAC GTCTC(1/5)
12 GTCTC
BsmFI 1025 GTCCC GGGAC(10/14)
1041 GTCCC
BspLU11I 368 ACATGT A^CATGT
1277 ACATGT
BspMI 38 ACCTGC ACCTGC(4/8)
391 ACCTGC
BstYI 713 AGATCC [GA]^GATC[CT]
1104 GGATCC
CfoI 873 GCGC GCG^C
1242 GCGC
EarI 909 CTCTTC CTCTTC(1/4)
1018 CTCTTC
HhaI 873 GCGC GCG^C
1242 GCGC
HinP1I 873 GCGC G^CGC
1242 GCGC
Ksp632I 909 CTCTTC CTCTTC(1/4)
1018 CTCTTC
MflI 713 AGATCC [GA]^GATC[CT]
1104 GGATCC
PflMI 203 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
579 CCAGGACCTGG
PpuMI 215 AGGACCC [GA]G^G[AT]CC[CT]
581 AGGACCT
PshAI 217 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
367 GACATGTGTC
PstI 348 CTGCAG CTGCA^G
475 CTGCAG
SapI 908 GCTCTTC GCTCTTC(1/4)
1017 GCTCTTC
TaqI 124 TCGA T^CGA
981 TCGA
Tsp509I 1069 AATT ^AATT
1223 AATT
TthHB8I 124 TCGA T^CGA
981 TCGA
Van91I 203 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
579 CCAGGACCTGG
XhoII 713 AGATCC [GA]^GATC[CT]
1104 GGATCC
XmnI 1218 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1405 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TseI Tsp45I TspRI