Restriction map for
pFN21AE1563
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AcsI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiEI
BsiWI BsmBI BsmFI BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HindIII KasI KpnI KspI
MamI MfeI MflI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 320 TCCGGA T^CCGGA
AclI 1224 AACGTT AA^CGTT
AhdI 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 600 GATCC GGATC(4/5)
ApaI 420 GGGCCC GGGCC^C
Asp700I 1006 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 19 GTCTTC GAAGAC(2/6)
BcgI 402 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 442 CTAG C^TAG
BpmI 187 CTGGAG CTGGAG(16/14)
BpuAI 19 GTCTTC GAAGAC(2/6)
BsaAI 181 TACGTG [CT]AC^GT[GA]
BsaI 586 GAGACC GGTCTC(1/5)
BseAI 320 TCCGGA T^CCGGA
BsgI 1315 GTGCAG GTGCAG(16/14)
Bsp1407I 104 TGTACA T^GTACA
BspEI 320 TCCGGA T^CCGGA
BsrGI 104 TGTACA T^GTACA
BssSI 214 CACGAG CACGAG(-5/-1)
BstXI 556 CCAGACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1002 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 99 GACGC GACGC(5/10)
HincII 1231 GTTAAC GT[CT]^[GA]AC
HindII 1231 GTTAAC GT[CT]^[GA]AC
HpaI 1231 GTTAAC GTT^AAC
MaeI 442 CTAG C^TAG
MroI 320 TCCGGA T^CCGGA
NcoI 1116 CCATGG C^CATGG
NspI 251 ACATGC [GA]CATG^[CT]
PleI 1301 GAGTC GAGTC(4/5)
PpuMI 1209 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1224 AACGTT AA^CGTT
SacI 1280 GAGCTC GAGCT^C
SapI 747 GCTCTTC GCTCTTC(1/4)
SmaI 590 CCCGGG CCC^GGG
SmlI 1259 CTCAAG C^T[CT][GA]AG
SspBI 104 TGTACA T^GTACA
TfiI 209 GAATC G^A[AT]TC
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
XmnI 1006 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 142 GTCTAC GT^[AC][GT]AC
769 GTCTAC
AfaI 105 GTAC GT^AC
525 GTAC
AspHI 691 GAGCAC G[AT]GC[AT]^C
1280 GAGCTC
AvaI 583 CCCGAG C^[CT]CG[GA]G
590 CCCGGG
BalI 662 TGGCCA TGG^CCA
761 TGGCCA
BsaWI 320 TCCGGA [AT]^CCGG[AT]
602 TCCGGT
BsiHKAI 691 GAGCAC G[AT]GC[AT]^C
1280 GAGCTC
BsoBI 583 CCCGAG C^[CT]CG[GA]G
590 CCCGGG
BspMI 8 ACCTGC ACCTGC(4/8)
545 ACCTGC
BsrI 563 ACTGG ACTGG(1/-1)
996 CCAGT
BstEII 60 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
BstPI 60 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
EaeI 662 TGGCCA [CT]^GGCC[GA]
761 TGGCCA
EcoO65I 60 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
FauI 138 GCGGG CCCGC(4/6)
963 GCGGG
HinfI 209 GAATC G^A[ACGT]TC
1301 GAGTC
MaeII 182 ACGT A^CGT
1225 ACGT
MluNI 662 TGGCCA TGG^CCA
761 TGGCCA
MscI 662 TGGCCA TGG^CCA
761 TGGCCA
MslI 76 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
782 CACATGGATG
PflMI 753 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAGTTCCTGG
PstI 289 CTGCAG CTGCA^G
1357 CTGCAG
PvuII 292 CAGCTG CAG^CTG
1354 CAGCTG
RsaI 105 GTAC GT^AC
525 GTAC
TspRI 1083 CAGTG CA[GC]TG[ACGT][ACGT]^
1155 CACTG
Van91I 753 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAGTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I