Restriction map for
pFN21AE3666
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI MaeI MaeII
MfeI MluI MroI MunI NarI
NciI NdeI NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflII 106 CTTAAG C^TTAAG
AflIII 973 ACATGT A^C[GA][CT]GT
AlwNI 697 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1044 CCTAAGG CC^T[ACGT]AGG
BalI 609 TGGCCA TGG^CCA
BbsI 196 GAAGAC GAAGAC(2/6)
BclI 257 TGATCA T^GATCA
BfrI 106 CTTAAG C^TTAAG
BmyI 538 GTGCCC G[AGT]GC[ACT]^C
BpmI 360 CTCCAG CTGGAG(16/14)
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaBI 931 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 348 GGTCTC GGTCTC(1/5)
BsiEI 616 CGATCG CG[GA][CT]^CG
BsmFI 80 GGGAC GGGAC(10/14)
Bsp1286I 538 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 973 ACATGT A^CATGT
BspMI 369 GCAGGT ACCTGC(4/8)
BsrI 127 ACTGG ACTGG(1/-1)
BssHII 94 GCGCGC G^CGCGC
BstXI 174 CCATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1044 CCTAAGG CC^T[ACGT]AGG
DraI 751 TTTAAA TTT^AAA
DraII 698 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 68 GACAGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1044 CCTAAGG CC^T[ACGT]AGG
EcoO109I 698 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 174 CCATGG C^C[AT][AT]GG
FbaI 257 TGATCA T^GATCA
HindIII 919 AAGCTT A^AGCTT
KspI 23 CCGCGG CCGC^GG
MamI 931 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 609 TGGCCA TGG^CCA
MscI 609 TGGCCA TGG^CCA
MslI 240 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4 GCCGGC GCC^GGC
NcoI 174 CCATGG C^CATGG
NgoMIV 4 GCCGGC G^CCGGC
NspI 973 ACATGT [GA]CATG^[CT]
PflMI 696 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 698 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 616 CGATCG CGAT^CG
PvuII 442 CAGCTG CAG^CTG
SacII 23 CCGCGG CCGC^GG
SexAI 695 ACCAGGT A^CC[AT]GGT
SmlI 106 CTTAAG C^T[CT][GA]AG
StyI 174 CCATGG C^C[AT][AT]GG
TaqI 62 TCGA T^CGA
TspRI 640 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 62 TCGA T^CGA
Van91I 696 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 783 GAATTC [GA]^AATT[CT]
1051 GAATTC
AfaI 438 GTAC GT^AC
840 GTAC
AlwI 400 GATCC GGATC(4/5)
816 GGATC
ApoI 783 GAATTC [GA]^AATT[CT]
1051 GAATTC
AspI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
694 GACCAGGTC
AvaII 699 GGTCC G^G[AT]CC
877 GGACC
BciVI 281 GGATAC GTATCC(6/5)
561 GTATCC
BseRI 28 GAGGAG GAGGAG(10/8)
429 GAGGAG
BsmAI 905 GAGAC GTCTC(1/5)
349 GTCTC
BsrFI 4 GCCGGC [GA]^CCGG[CT]
547 ACCGGC
BstYI 399 AGATCC [GA]^GATC[CT]
816 GGATCT
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
547 ACCGGC
Cfr13I 699 GGTCC G^G[ACGT]CC
877 GGACC
DsaI 23 CCGCGG C^C[GA][CT]GG
174 CCATGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
609 TGGCCA
EarI 733 GAAGAG CTCTTC(1/4)
826 GAAGAG
EcoRI 783 GAATTC G^AATTC
1051 GAATTC
HapII 5 CCGG C^CGG
548 CCGG
HgaI 91 GACGC GACGC(5/10)
530 GCGTC
HpaII 5 CCGG C^CGG
548 CCGG
Ksp632I 733 GAAGAG CTCTTC(1/4)
826 GAAGAG
MflI 399 AGATCC [GA]^GATC[CT]
816 GGATCT
MseI 107 TTAA T^TAA
752 TTAA
MspA1I 23 CCGCGG C[AC]G^C[GT]G
442 CAGCTG
MspI 5 CCGG C^CGG
548 CCGG
PflFI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
694 GACCAGGTC
PleI 451 GACTC GAGTC(4/5)
37 GAGTC
RsaI 438 GTAC GT^AC
840 GTAC
Sau96I 699 GGTCC G^G[ACGT]CC
877 GGACC
SfcI 980 CTACAG C^T[GA][CT]AG
1033 CTACAG
SspI 461 AATATT AAT^ATT
994 AATATT
Tru9I 107 TTAA T^TAA
752 TTAA
Tsp45I 655 GTCAC ^GT[GC]AC
713 GTGAC
Tth111I 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
694 GACCAGGTC
XcmI 362 CCAGGGAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
662 CCATTATTTTCTTGG
XhoII 399 AGATCC [GA]^GATC[CT]
816 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstUI Cac8I CfoI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MvnI
MwoI NdeII NlaIII Sau3AI ScrFI
SfaNI TfiI TseI Tsp509I