Restriction map for
pFN21AE3641
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbeI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MamI
MluI MroI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflMI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 1183 GACGCC G[GA]^CG[CT]C
AgeI 34 ACCGGT A^CCGGT
AspI 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 275 TGGCCA TGG^CCA
BamHI 1500 GGATCC G^GATCC
BbrPI 1189 CACGTG CAC^GTG
BbsI 204 GAAGAC GAAGAC(2/6)
BcgI 830 CGACACGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 310 CTAG C^TAG
BpuAI 204 GAAGAC GAAGAC(2/6)
BsaAI 1189 CACGTG [CT]AC^GT[GA]
BsaHI 1183 GACGCC G[GA]^CG[CT]C
BsaI 1327 GAGACC GGTCTC(1/5)
BsaWI 34 ACCGGT [AT]^CCGG[AT]
BsgI 1339 CTGCAC GTGCAG(16/14)
BstEII 1020 GGTGACC G^GT[ACGT]ACC
BstPI 1020 GGTGACC G^GT[ACGT]ACC
BstXI 51 CCAGACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1015 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1020 GGTGACC G^GT[ACGT]ACC
HaeII 421 AGCGCC [GA]GCGC^[CT]
Hin1I 1183 GACGCC G[GA]^CG[CT]C
KspI 671 CCGCGG CCGC^GG
MaeI 310 CTAG C^TAG
MaeII 1190 ACGT A^CGT
MfeI 533 CAATTG C^AATTG
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MseI 815 TTAA T^TAA
MunI 533 CAATTG C^AATTG
NaeI 1465 GCCGGC GCC^GGC
NgoMIV 1465 GCCGGC G^CCGGC
PaeR7I 236 CTCGAG C^TCGAG
PflFI 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 34 ACCGGT A^CCGGT
PleI 1489 GACTC GAGTC(4/5)
PmaCI 1189 CACGTG CAC^GTG
PmlI 1189 CACGTG CAC^GTG
PshAI 733 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 469 GAGCTC GAGCT^C
SacII 671 CCGCGG CCGC^GG
ScaI 315 AGTACT AGT^ACT
SexAI 1016 ACCAGGT A^CC[AT]GGT
SfiI 489 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 962 GATTC G^A[AT]TC
Tru9I 815 TTAA T^TAA
Tth111I 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 138 CCAGGATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 236 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 660 CGCG CG^CG
672 CGCG
AcsI 762 GAATTT [GA]^AATT[CT]
1147 AAATTC
AhdI 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1243 GACTTTGGGTC
Alw44I 544 GTGCAC G^TGCAC
825 GTGCAC
ApaLI 544 GTGCAC G^TGCAC
825 GTGCAC
ApoI 762 GAATTT [GA]^AATT[CT]
1147 AAATTC
AspEI 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1243 GACTTTGGGTC
AvaI 236 CTCGAG C^[CT]CG[GA]G
1321 CCCGAG
BbvCI 385 CCTCAGC CCTCAGC(-5/-2)
558 GCTGAGG
BpmI 1008 CTCCAG CTGGAG(16/14)
857 CTGGAG
BsmI 614 GCATTC GAATGC(1/-1)
1174 GCATTC
BsoBI 236 CTCGAG C^[CT]CG[GA]G
1321 CCCGAG
BsrBI 231 CCGCTC CCGCTC(-3/-3)
406 GAGCGG
BstF5I 1284 CATCC GGATG(2/0)
141 GGATG
BstUI 660 CGCG CG^CG
672 CGCG
BstYI 587 AGATCC [GA]^GATC[CT]
1500 GGATCC
CfoI 422 GCGC GCG^C
659 GCGC
DsaI 671 CCGCGG C^C[GA][CT]GG
809 CCGTGG
Eam1105I 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1243 GACTTTGGGTC
EarI 1408 CTCTTC CTCTTC(1/4)
463 GAAGAG
FokI 1284 CATCC GGATG(9/13)
141 GGATG
HhaI 422 GCGC GCG^C
659 GCGC
HinP1I 422 GCGC G^CGC
659 GCGC
HinfI 962 GATTC G^A[ACGT]TC
1489 GACTC
Ksp632I 1408 CTCTTC CTCTTC(1/4)
463 GAAGAG
MflI 587 AGATCC [GA]^GATC[CT]
1500 GGATCC
MslI 1080 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1284 CATCCCGGTG
MvnI 660 CGCG CG^CG
672 CGCG
SbfI 696 CCTGCAGG CCTGCA^GG
1397 CCTGCAGG
SmlI 236 CTCGAG C^T[CT][GA]AG
649 CTCAAG
Sse8387I 696 CCTGCAGG CCTGCA^GG
1397 CCTGCAGG
StuI 156 AGGCCT AGG^CCT
701 AGGCCT
TaqI 237 TCGA T^CGA
1082 TCGA
TthHB8I 237 TCGA T^CGA
1082 TCGA
XhoII 587 AGATCC [GA]^GATC[CT]
1500 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI