Restriction map for
pFN21AE2234
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BanI BbeI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BpmI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI HapII HgaI Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 193 GAATTT [GA]^AATT[CT]
AlwNI 643 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 263 AGCGCT AGC^GCT
ApoI 193 GAATTT [GA]^AATT[CT]
AspHI 583 GAGCAC G[AT]GC[AT]^C
AvaII 758 GGTCC G^G[AT]CC
BalI 729 TGGCCA TGG^CCA
BamHI 162 GGATCC G^GATCC
BbrPI 441 CACGTG CAC^GTG
BfaI 737 CTAG C^TAG
BlpI 30 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTCAGC GC^T[ACGT]AGC
BsaAI 441 CACGTG [CT]AC^GT[GA]
BsgI 42 CTGCAC GTGCAG(16/14)
BsiHKAI 583 GAGCAC G[AT]GC[AT]^C
BsmAI 509 GAGAC GTCTC(1/5)
BsrDI 272 GCAATG GCAATG(2/0)
BssSI 378 CACGAG CACGAG(-5/-1)
BstAPI 261 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 498 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 30 GCTCAGC GC^T[ACGT]AGC
EarI 749 CTCTTC CTCTTC(1/4)
Eco47III 263 AGCGCT AGC^GCT
HaeII 263 AGCGCT [GA]GCGC^[CT]
HindIII 460 AAGCTT A^AGCTT
Ksp632I 749 CTCTTC CTCTTC(1/4)
MaeI 737 CTAG C^TAG
MaeII 442 ACGT A^CGT
MluNI 729 TGGCCA TGG^CCA
MscI 729 TGGCCA TGG^CCA
PmaCI 441 CACGTG CAC^GTG
PmlI 441 CACGTG CAC^GTG
PstI 665 CTGCAG CTGCA^G
PvuII 714 CAGCTG CAG^CTG
SfcI 665 CTGCAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 16 CCGC CCGC(-3/-1)
720 GCGG
AviII 269 TGCGCA TGC^GCA
483 TGCGCA
BclI 2 TGATCA T^GATCA
710 TGATCA
BseRI 746 CTCCTC GAGGAG(10/8)
169 GAGGAG
BspMI 154 ACCTGC ACCTGC(4/8)
52 GCAGGT
BsrI 512 ACTGG ACTGG(1/-1)
617 ACTGG
BstYI 144 GGATCT [GA]^GATC[CT]
162 GGATCC
DdeI 31 CTCAG C^T[ACGT]AG
599 CTGAG
EaeI 337 CGGCCA [CT]^GGCC[GA]
729 TGGCCA
FbaI 2 TGATCA T^GATCA
710 TGATCA
FspI 269 TGCGCA TGC^GCA
483 TGCGCA
HinfI 105 GACTC G^A[ACGT]TC
672 GACTC
MflI 144 GGATCT [GA]^GATC[CT]
162 GGATCC
MseI 374 TTAA T^TAA
544 TTAA
MspA1I 16 CCGCTG C[AC]G^C[GT]G
714 CAGCTG
PleI 105 GACTC GAGTC(4/5)
672 GACTC
Tru9I 374 TTAA T^TAA
544 TTAA
Tsp45I 433 GTGAC ^GT[GC]AC
670 GTGAC
XhoII 144 GGATCT [GA]^GATC[CT]
162 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I ItaI MaeIII
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I
TspRI