Restriction map for
pFN21AE6431
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 348 GACGTC GACGT^C
AlwNI 739 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1090 GAGCTC G[AT]GC[AT]^C
AvaI 254 CTCGGG C^[CT]CG[GA]G
BanI 1077 GGTGCC G^G[CT][GA]CC
BbsI 688 GAAGAC GAAGAC(2/6)
BclI 809 TGATCA T^GATCA
BcnI 24 CCGGG CC^[GC]GG
BpuAI 688 GAAGAC GAAGAC(2/6)
BsiEI 109 CGGTCG CG[GA][CT]^CG
BsiHKAI 1090 GAGCTC G[AT]GC[AT]^C
BsmAI 126 GTCTC GTCTC(1/5)
BsoBI 254 CTCGGG C^[CT]CG[GA]G
BsrBI 67 CCGCTC CCGCTC(-3/-3)
BstXI 1190 CCAACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 825 GCGC GCG^C
CpoI 20 CGGACCG CG^G[AT]CCG
DraI 1273 TTTAAA TTT^AAA
EcoRV 1051 GATATC GAT^ATC
FauI 66 CCCGC CCCGC(4/6)
FbaI 809 TGATCA T^GATCA
HhaI 825 GCGC GCG^C
HinP1I 825 GCGC G^CGC
MfeI 213 CAATTG C^AATTG
MslI 1008 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 895 CAGCTG C[AC]G^C[GT]G
MunI 213 CAATTG C^AATTG
NciI 24 CCGGG CC^[GC]GG
PstI 13 CTGCAG CTGCA^G
PvuII 895 CAGCTG CAG^CTG
RsrII 20 CGGACCG CG^G[AT]CCG
SacI 1090 GAGCTC GAGCT^C
SmlI 1145 CTCAAG C^T[CT][GA]AG
SphI 236 GCATGC GCATG^C
SspI 446 AATATT AAT^ATT
TaqI 1025 TCGA T^CGA
TfiI 929 GAATC G^A[AT]TC
TspRI 1062 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1025 TCGA T^CGA
XbaI 352 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
348 GACGTC
AfaI 593 GTAC GT^AC
1125 GTAC
AlwI 1162 GATCC GGATC(4/5)
627 GGATC
ApaI 62 GGGCCC GGGCC^C
1365 GGGCCC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
348 GACGTC
BsaJI 42 CCAGGG C^C[ACGT][ACGT]GG
1317 CCAGGG
BseRI 1033 GAGGAG GAGGAG(10/8)
1231 GAGGAG
BsiYI 571 CCACGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1344 CCAGCATGTGG
BslI 571 CCACGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1344 CCAGCATGTGG
DraIII 130 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1223 CACAGCGTG
HapII 24 CCGG C^CGG
602 CCGG
HgaI 4 GCGTC GACGC(5/10)
741 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
348 GACGTC
HpaII 24 CCGG C^CGG
602 CCGG
MspI 24 CCGG C^CGG
602 CCGG
MwoI 92 GCTATTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1338 GCTCACCCAGC
PflMI 571 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1344 CCAGCATGTGG
PpuMI 971 AGGTCCT [GA]G^G[AT]CC[CT]
1040 GGGACCC
RsaI 593 GTAC GT^AC
1125 GTAC
SfcI 13 CTGCAG C^T[GA][CT]AG
380 CTGTAG
Van91I 571 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1344 CCAGCATGTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanII BbvI BfaI BmyI BsmFI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I