################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I FseI FspI HapII HpaI HpaII KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 983 GAATTC [GA]^AATT[CT] AflIII 1241 ACATGT A^C[GA][CT]GT AlwNI 751 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1165 AGCGCT AGC^GCT ApoI 983 GAATTC [GA]^AATT[CT] AseI 745 ATTAAT AT^TAAT AsnI 745 ATTAAT AT^TAAT BanI 483 GGCGCC G^G[CT][GA]CC BbeI 483 GGCGCC GGCGC^C BclI 812 TGATCA T^GATCA BlpI 1107 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1107 GCTCAGC GC^T[ACGT]AGC BsaI 1393 GAGACC GGTCTC(1/5) BseRI 1483 GAGGAG GAGGAG(10/8) BsmFI 948 GGGAC GGGAC(10/14) BsmI 1192 GAATGC GAATGC(1/-1) BspHI 263 TCATGA T^CATGA BspLU11I 1241 ACATGT A^CATGT BstEII 1179 GGTGACC G^GT[ACGT]ACC BstPI 1179 GGTGACC G^GT[ACGT]ACC CelII 1107 GCTCAGC GC^T[ACGT]AGC DsaI 449 CCATGG C^C[GA][CT]GG Eco47III 1165 AGCGCT AGC^GCT EcoNI 489 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1179 GGTGACC G^GT[ACGT]ACC EcoRI 983 GAATTC G^AATTC EcoRV 180 GATATC GAT^ATC EcoT22I 101 ATGCAT ATGCA^T FauI 1078 GCGGG CCCGC(4/6) FbaI 812 TGATCA T^GATCA HgaI 1280 GCGTC GACGC(5/10) HincII 922 GTTGAC GT[CT]^[GA]AC HindII 922 GTTGAC GT[CT]^[GA]AC HindIII 528 AAGCTT A^AGCTT KasI 483 GGCGCC G^GCGCC MaeII 338 ACGT A^CGT MslI 1314 CATTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 281 GCTTGTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 483 GGCGCC GG^CGCC NcoI 449 CCATGG C^CATGG NdeI 1030 CATATG CA^TATG NheI 1035 GCTAGC G^CTAGC NsiI 101 ATGCAT ATGCA^T PflMI 7 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 216 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 745 ATTAAT AT^TAAT RcaI 263 TCATGA T^CATGA SfoI 483 GGCGCC GGC^GCC SmlI 640 CTTGAG C^T[CT][GA]AG Tsp45I 1180 GTGAC ^GT[GC]AC TspRI 62 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 7 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 40 CCAGATGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AciI 1000 GCGG CCGC(-3/-1) 1078 GCGG AcyI 483 GGCGCC G[GA]^CG[CT]C 1279 GGCGTC AfaI 325 GTAC GT^AC 476 GTAC ApaI 595 GGGCCC GGGCC^C 1474 GGGCCC BalI 434 TGGCCA TGG^CCA 446 TGGCCA BciVI 28 GGATAC GTATCC(6/5) 230 GTATCC BfaI 881 CTAG C^TAG 1036 CTAG BglII 672 AGATCT A^GATCT 693 AGATCT BsaHI 483 GGCGCC G[GA]^CG[CT]C 1279 GGCGTC BsmAI 1250 GAGAC GTCTC(1/5) 1393 GAGAC BstF5I 635 CATCC GGATG(2/0) 517 GGATG BstYI 672 AGATCT [GA]^GATC[CT] 693 AGATCT CfoI 484 GCGC GCG^C 1166 GCGC DdeI 850 CTTAG C^T[ACGT]AG 1108 CTCAG DraII 216 AGGTCCT [GA]G^G[ACGT]CC[CT] 1473 AGGGCCC EaeI 434 TGGCCA [CT]^GGCC[GA] 446 TGGCCA EcoO109I 216 AGGTCCT [GA]G^G[ACGT]CC[CT] 1473 AGGGCCC FokI 635 CATCC GGATG(9/13) 517 GGATG HaeII 483 GGCGCC [GA]GCGC^[CT] 1165 AGCGCT HhaI 484 GCGC GCG^C 1166 GCGC Hin1I 483 GGCGCC G[GA]^CG[CT]C 1279 GGCGTC HinP1I 484 GCGC G^CGC 1166 GCGC MaeI 881 CTAG C^TAG 1036 CTAG MflI 672 AGATCT [GA]^GATC[CT] 693 AGATCT MluNI 434 TGGCCA TGG^CCA 446 TGGCCA MscI 434 TGGCCA TGG^CCA 446 TGGCCA MseI 746 TTAA T^TAA 763 TTAA NspI 1241 ACATGT [GA]CATG^[CT] 1273 GCATGT RsaI 325 GTAC GT^AC 476 GTAC TaqI 981 TCGA T^CGA 1335 TCGA Tru9I 746 TTAA T^TAA 763 TTAA TthHB8I 981 TCGA T^CGA 1335 TCGA XhoII 672 AGATCT [GA]^GATC[CT] 693 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstXI Cac8I Cfr13I DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I