Restriction map for
pFN21AE4484
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I FseI FspI HapII
HpaI HpaII KpnI KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NciI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 983 GAATTC [GA]^AATT[CT]
AflIII 1241 ACATGT A^C[GA][CT]GT
AlwNI 751 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1165 AGCGCT AGC^GCT
ApoI 983 GAATTC [GA]^AATT[CT]
AseI 745 ATTAAT AT^TAAT
AsnI 745 ATTAAT AT^TAAT
BanI 483 GGCGCC G^G[CT][GA]CC
BbeI 483 GGCGCC GGCGC^C
BclI 812 TGATCA T^GATCA
BlpI 1107 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1107 GCTCAGC GC^T[ACGT]AGC
BsaI 1393 GAGACC GGTCTC(1/5)
BseRI 1483 GAGGAG GAGGAG(10/8)
BsmFI 948 GGGAC GGGAC(10/14)
BsmI 1192 GAATGC GAATGC(1/-1)
BspHI 263 TCATGA T^CATGA
BspLU11I 1241 ACATGT A^CATGT
BstEII 1179 GGTGACC G^GT[ACGT]ACC
BstPI 1179 GGTGACC G^GT[ACGT]ACC
CelII 1107 GCTCAGC GC^T[ACGT]AGC
DsaI 449 CCATGG C^C[GA][CT]GG
Eco47III 1165 AGCGCT AGC^GCT
EcoNI 489 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1179 GGTGACC G^GT[ACGT]ACC
EcoRI 983 GAATTC G^AATTC
EcoRV 180 GATATC GAT^ATC
EcoT22I 101 ATGCAT ATGCA^T
FauI 1078 GCGGG CCCGC(4/6)
FbaI 812 TGATCA T^GATCA
HgaI 1280 GCGTC GACGC(5/10)
HincII 922 GTTGAC GT[CT]^[GA]AC
HindII 922 GTTGAC GT[CT]^[GA]AC
HindIII 528 AAGCTT A^AGCTT
KasI 483 GGCGCC G^GCGCC
MaeII 338 ACGT A^CGT
MslI 1314 CATTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 281 GCTTGTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 483 GGCGCC GG^CGCC
NcoI 449 CCATGG C^CATGG
NdeI 1030 CATATG CA^TATG
NheI 1035 GCTAGC G^CTAGC
NsiI 101 ATGCAT ATGCA^T
PflMI 7 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 216 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 745 ATTAAT AT^TAAT
RcaI 263 TCATGA T^CATGA
SfoI 483 GGCGCC GGC^GCC
SmlI 640 CTTGAG C^T[CT][GA]AG
Tsp45I 1180 GTGAC ^GT[GC]AC
TspRI 62 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 7 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 40 CCAGATGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 1000 GCGG CCGC(-3/-1)
1078 GCGG
AcyI 483 GGCGCC G[GA]^CG[CT]C
1279 GGCGTC
AfaI 325 GTAC GT^AC
476 GTAC
ApaI 595 GGGCCC GGGCC^C
1474 GGGCCC
BalI 434 TGGCCA TGG^CCA
446 TGGCCA
BciVI 28 GGATAC GTATCC(6/5)
230 GTATCC
BfaI 881 CTAG C^TAG
1036 CTAG
BglII 672 AGATCT A^GATCT
693 AGATCT
BsaHI 483 GGCGCC G[GA]^CG[CT]C
1279 GGCGTC
BsmAI 1250 GAGAC GTCTC(1/5)
1393 GAGAC
BstF5I 635 CATCC GGATG(2/0)
517 GGATG
BstYI 672 AGATCT [GA]^GATC[CT]
693 AGATCT
CfoI 484 GCGC GCG^C
1166 GCGC
DdeI 850 CTTAG C^T[ACGT]AG
1108 CTCAG
DraII 216 AGGTCCT [GA]G^G[ACGT]CC[CT]
1473 AGGGCCC
EaeI 434 TGGCCA [CT]^GGCC[GA]
446 TGGCCA
EcoO109I 216 AGGTCCT [GA]G^G[ACGT]CC[CT]
1473 AGGGCCC
FokI 635 CATCC GGATG(9/13)
517 GGATG
HaeII 483 GGCGCC [GA]GCGC^[CT]
1165 AGCGCT
HhaI 484 GCGC GCG^C
1166 GCGC
Hin1I 483 GGCGCC G[GA]^CG[CT]C
1279 GGCGTC
HinP1I 484 GCGC G^CGC
1166 GCGC
MaeI 881 CTAG C^TAG
1036 CTAG
MflI 672 AGATCT [GA]^GATC[CT]
693 AGATCT
MluNI 434 TGGCCA TGG^CCA
446 TGGCCA
MscI 434 TGGCCA TGG^CCA
446 TGGCCA
MseI 746 TTAA T^TAA
763 TTAA
NspI 1241 ACATGT [GA]CATG^[CT]
1273 GCATGT
RsaI 325 GTAC GT^AC
476 GTAC
TaqI 981 TCGA T^CGA
1335 TCGA
Tru9I 746 TTAA T^TAA
763 TTAA
TthHB8I 981 TCGA T^CGA
1335 TCGA
XhoII 672 AGATCT [GA]^GATC[CT]
693 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AvaII BanII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstXI Cac8I Cfr13I DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI Tsp509I