Restriction map for
pFN21AE4430
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 967 GACGTC GACGT^C
Alw44I 562 GTGCAC G^TGCAC
ApaLI 562 GTGCAC G^TGCAC
AvrII 1102 CCTAGG C^CTAGG
BalI 1334 TGGCCA TGG^CCA
BamHI 655 GGATCC G^GATCC
BbsI 1057 GAAGAC GAAGAC(2/6)
BcnI 1238 CCCGG CC^[GC]GG
BfaI 1103 CTAG C^TAG
BlnI 1102 CCTAGG C^CTAGG
BpuAI 1057 GAAGAC GAAGAC(2/6)
BsaI 798 GGTCTC GGTCTC(1/5)
BsgI 1243 CTGCAC GTGCAG(16/14)
BsmBI 693 CGTCTC CGTCTC(1/5)
BspMI 611 ACCTGC ACCTGC(4/8)
BsrBI 472 GAGCGG CCGCTC(-3/-3)
BstXI 196 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 655 GGATCC [GA]^GATC[CT]
EaeI 1334 TGGCCA [CT]^GGCC[GA]
FauI 474 GCGGG CCCGC(4/6)
HapII 1239 CCGG C^CGG
HgaI 849 GCGTC GACGC(5/10)
HpaII 1239 CCGG C^CGG
MaeI 1103 CTAG C^TAG
MflI 655 GGATCC [GA]^GATC[CT]
MluNI 1334 TGGCCA TGG^CCA
MscI 1334 TGGCCA TGG^CCA
MslI 622 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1239 CCGG C^CGG
NciI 1238 CCCGG CC^[GC]GG
NspI 59 ACATGC [GA]CATG^[CT]
PstI 704 CTGCAG CTGCA^G
TfiI 245 GATTC G^A[AT]TC
XhoII 655 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 288 CCGC CCGC(-3/-1)
474 GCGG
AcsI 535 GAATTC [GA]^AATT[CT]
1144 GAATTC
AcyI 848 GGCGTC G[GA]^CG[CT]C
967 GACGTC
AfaI 171 GTAC GT^AC
778 GTAC
ApaI 45 GGGCCC GGGCC^C
70 GGGCCC
ApoI 535 GAATTC [GA]^AATT[CT]
1144 GAATTC
AvaI 31 CTCGGG C^[CT]CG[GA]G
164 CTCGGG
BclI 113 TGATCA T^GATCA
464 TGATCA
BglI 305 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1327 GCCACTCTGGC
BsaHI 848 GGCGTC G[GA]^CG[CT]C
967 GACGTC
BsoBI 31 CTCGGG C^[CT]CG[GA]G
164 CTCGGG
EarI 826 CTCTTC CTCTTC(1/4)
1174 GAAGAG
EcoRI 535 GAATTC G^AATTC
1144 GAATTC
FbaI 113 TGATCA T^GATCA
464 TGATCA
Hin1I 848 GGCGTC G[GA]^CG[CT]C
967 GACGTC
Ksp632I 826 CTCTTC CTCTTC(1/4)
1174 GAAGAG
PflMI 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCACTGTCTGG
RsaI 171 GTAC GT^AC
778 GTAC
SacI 515 GAGCTC GAGCT^C
640 GAGCTC
SfaNI 495 GCATC GCATC(5/9)
1207 GCATC
Tsp509I 536 AATT ^AATT
1145 AATT
Van91I 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCACTGTCTGG
XcmI 326 CCAGTGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
626 CCAGTGACCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AluI AlwI AlwNI AocI AspHI
AvaII BanII BbvI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraII Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PvuII
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I TspRI