Restriction map for
pFN21AE2622
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 423 TCCGGA T^CCGGA
ApaI 4 GGGCCC GGGCC^C
AspI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 944 TGATCA T^GATCA
BcnI 555 CCCGG CC^[GC]GG
BfaI 995 CTAG C^TAG
BglI 1030 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 100 GCTCAGC GC^T[ACGT]AGC
BpmI 447 CTCCAG CTGGAG(16/14)
Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC
BsaWI 423 TCCGGA [AT]^CCGG[AT]
BseAI 423 TCCGGA T^CCGGA
BsmFI 563 GTCCC GGGAC(10/14)
BspEI 423 TCCGGA T^CCGGA
BspHI 1013 TCATGA T^CATGA
BssSI 185 CTCGTG CACGAG(-5/-1)
BstXI 461 CCAACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 100 GCTCAGC GC^T[ACGT]AGC
CfoI 33 GCGC GCG^C
DsaI 467 CCGTGG C^C[GA][CT]GG
EcoT22I 311 ATGCAT ATGCA^T
FauI 87 CCCGC CCCGC(4/6)
FbaI 944 TGATCA T^GATCA
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
MaeI 995 CTAG C^TAG
MroI 423 TCCGGA T^CCGGA
NciI 555 CCCGG CC^[GC]GG
NsiI 311 ATGCAT ATGCA^T
PflFI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 930 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 574 CAGCTG CAG^CTG
RcaI 1013 TCATGA T^CATGA
ScaI 1023 AGTACT AGT^ACT
Tsp509I 234 AATT ^AATT
Tth111I 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 177 CCAGGACCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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BalI 405 TGGCCA TGG^CCA
1028 TGGCCA
BamHI 352 GGATCC G^GATCC
883 GGATCC
BanII 4 GGGCCC G[GA]GC[CT]^C
49 GGGCTC
BmyI 4 GGGCCC G[AGT]GC[ACT]^C
49 GGGCTC
Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C
49 GGGCTC
BstYI 352 GGATCC [GA]^GATC[CT]
883 GGATCC
Cac8I 276 GCCTGC GC[ACGT]^[ACGT]GC
892 GCCTGC
HapII 424 CCGG C^CGG
556 CCGG
HpaII 424 CCGG C^CGG
556 CCGG
MaeII 332 ACGT A^CGT
626 ACGT
MboII 394 GAAGA GAAGA(8/7)
153 TCTTC
MflI 352 GGATCC [GA]^GATC[CT]
883 GGATCC
MluNI 405 TGGCCA TGG^CCA
1028 TGGCCA
MscI 405 TGGCCA TGG^CCA
1028 TGGCCA
MspA1I 574 CAGCTG C[AC]G^C[GT]G
879 CAGCGG
MspI 424 CCGG C^CGG
556 CCGG
TaqI 231 TCGA T^CGA
291 TCGA
TthHB8I 231 TCGA T^CGA
291 TCGA
XhoII 352 GGATCC [GA]^GATC[CT]
883 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI