Restriction map for
pFN21AE4313
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NarI NciI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 636 GTCTAC GT^[AC][GT]AC
AfaI 500 GTAC GT^AC
Asp700I 789 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2 TGGCCA TGG^CCA
BpmI 560 CTCCAG CTGGAG(16/14)
BsgI 77 GTGCAG GTGCAG(16/14)
BspMI 534 ACCTGC ACCTGC(4/8)
BsrFI 205 GCCGGC [GA]^CCGG[CT]
BsrI 355 CCAGT ACTGG(1/-1)
BstYI 441 AGATCC [GA]^GATC[CT]
Cfr10I 205 GCCGGC [GA]^CCGG[CT]
EcoT14I 63 CCAAGG C^C[AT][AT]GG
HapII 206 CCGG C^CGG
HpaII 206 CCGG C^CGG
MflI 441 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 206 CCGG C^CGG
NaeI 205 GCCGGC GCC^GGC
NgoMIV 205 GCCGGC G^CCGGC
NheI 238 GCTAGC G^CTAGC
PstI 749 CTGCAG CTGCA^G
RsaI 500 GTAC GT^AC
SapI 82 GAAGAGC GCTCTTC(1/4)
StyI 63 CCAAGG C^C[AT][AT]GG
TaqI 833 TCGA T^CGA
TthHB8I 833 TCGA T^CGA
XcmI 47 CCACGAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 441 AGATCC [GA]^GATC[CT]
XmnI 789 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 156 GAATTT [GA]^AATT[CT]
481 GAATTT
AlwI 442 GATCC GGATC(4/5)
119 GGATC
AlwNI 148 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
641 CAGAAACTG
ApoI 156 GAATTT [GA]^AATT[CT]
481 GAATTT
BsmFI 192 GGGAC GGGAC(10/14)
706 GTCCC
BsmI 416 GCATTC GAATGC(1/-1)
673 GCATTC
BssSI 48 CACGAG CACGAG(-5/-1)
411 CTCGTG
BstF5I 93 CATCC GGATG(2/0)
515 CATCC
DdeI 170 CTAAG C^T[ACGT]AG
196 CTAAG
DsaI 580 CCACGG C^C[GA][CT]GG
710 CCACGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
203 TGGCCG
FokI 93 CATCC GGATG(9/13)
515 CATCC
HphI 122 TCACC GGTGA(8/7)
431 TCACC
MslI 93 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
123 CACCCTTGTG
MspA1I 151 CAGCTG C[AC]G^C[GT]G
752 CAGCTG
NlaIII 104 CATG CATG^
633 CATG
NlaIV 53 GGCTCC GG[ACGT]^[ACGT]CC
351 GGTTCC
PleI 916 GAGTC GAGTC(4/5)
924 GAGTC
PvuII 151 CAGCTG CAG^CTG
752 CAGCTG
SfaNI 947 GATGC GCATC(5/9)
514 GCATC
StuI 176 AGGCCT AGG^CCT
791 AGGCCT
Tsp45I 494 GTGAC ^GT[GC]AC
604 GTCAC
TspRI 356 CAGTG CA[GC]TG[ACGT][ACGT]^
492 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AvaII BbvI BfaI BsaJI
BseRI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MvaI MwoI NdeII Sau3AI Sau96I
ScrFI SfcI TfiI TseI Tsp509I