Restriction map for
pFN21AB5917
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbvCI BciVI
BclI BfrI BglI BlnI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluNI MroI MscI MseI
MslI MunI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 511 GACGTC GACGT^C
AccI 1230 GTCTAC GT^[AC][GT]AC
AfaI 1016 GTAC GT^AC
AlwI 1422 GATCC GGATC(4/5)
AocI 744 CCTGAGG CC^T[ACGT]AGG
ApaI 217 GGGCCC GGGCC^C
AscI 14 GGCGCGCC GG^CGCGCC
BbrPI 1242 CACGTG CAC^GTG
BcgI 658 GCATATGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 764 CTAG C^TAG
BlpI 1306 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1306 GCTGAGC GC^T[ACGT]AGC
BsaAI 1242 CACGTG [CT]AC^GT[GA]
BsaI 985 GAGACC GGTCTC(1/5)
BseRI 964 CTCCTC GAGGAG(10/8)
BsmBI 513 CGTCTC CGTCTC(1/5)
BspLU11I 641 ACATGT A^CATGT
BssSI 1440 CTCGTG CACGAG(-5/-1)
BstAPI 84 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 744 CCTGAGG CC^T[ACGT]AGG
CelII 1306 GCTGAGC GC^T[ACGT]AGC
EagI 312 CGGCCG C^GGCCG
EclXI 312 CGGCCG C^GGCCG
Eco52I 312 CGGCCG C^GGCCG
Eco81I 744 CCTGAGG CC^T[ACGT]AGG
MaeI 764 CTAG C^TAG
MluI 1114 ACGCGT A^CGCGT
NdeI 659 CATATG CA^TATG
NspI 641 ACATGT [GA]CATG^[CT]
PflMI 1182 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1242 CACGTG CAC^GTG
PmlI 1242 CACGTG CAC^GTG
RsaI 1016 GTAC GT^AC
SacI 1087 GAGCTC GAGCT^C
SgrAI 282 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 463 CTCAAG C^T[CT][GA]AG
Van91I 1182 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1182 CCAGCTTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 641 ACATGT A^C[GA][CT]GT
1114 ACGCGT
AlwNI 838 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1159 CAGGAGCTG
AviII 269 TGCGCA TGC^GCA
1103 TGCGCA
BglII 347 AGATCT A^GATCT
710 AGATCT
BsiEI 312 CGGCCG CG[GA][CT]^CG
551 CGGTCG
BspMI 1433 ACCTGC ACCTGC(4/8)
456 GCAGGT
BssHII 15 GCGCGC G^CGCGC
45 GCGCGC
BstXI 698 CCAGGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1034 CCACCAGACTGG
DraIII 1120 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1293 CACACTGTG
EarI 739 CTCTTC CTCTTC(1/4)
669 GAAGAG
EcoT14I 752 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
FspI 269 TGCGCA TGC^GCA
1103 TGCGCA
Ksp632I 739 CTCTTC CTCTTC(1/4)
669 GAAGAG
NaeI 67 GCCGGC GCC^GGC
1004 GCCGGC
NgoMIV 67 GCCGGC G^CCGGC
1004 GCCGGC
NlaIII 442 CATG CATG^
642 CATG
PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT]
544 GGGACCT
PshAI 142 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
546 GACCTCGGTC
StyI 752 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
ApaLI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIV PleI PstI PvuII
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI TaqI TseI Tsp45I
TspRI TthHB8I XhoII