Restriction map for
pFN21AE1427
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI BcgI BclI BfrI BglII
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 3770 TCCGGA T^CCGGA
AcyI 2907 GGCGCC G[GA]^CG[CT]C
AflIII 14 ACGTGT A^C[GA][CT]GT
AhdI 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3341 TGCGCA TGC^GCA
AvrII 3880 CCTAGG C^CTAGG
BbeI 2907 GGCGCC GGCGC^C
BbrPI 13 CACGTG CAC^GTG
BlnI 3880 CCTAGG C^CTAGG
BsaAI 13 CACGTG [CT]AC^GT[GA]
BsaHI 2907 GGCGCC G[GA]^CG[CT]C
BseAI 3770 TCCGGA T^CCGGA
Bsp1407I 53 TGTACA T^GTACA
BspEI 3770 TCCGGA T^CCGGA
BsrGI 53 TGTACA T^GTACA
BstEII 2442 GGTGACC G^GT[ACGT]ACC
BstPI 2442 GGTGACC G^GT[ACGT]ACC
DrdI 2423 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2442 GGTGACC G^GT[ACGT]ACC
EcoRI 3366 GAATTC G^AATTC
FspI 3341 TGCGCA TGC^GCA
Hin1I 2907 GGCGCC G[GA]^CG[CT]C
KasI 2907 GGCGCC G^GCGCC
MroI 3770 TCCGGA T^CCGGA
MseI 680 TTAA T^TAA
MslI 2377 CATTCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2907 GGCGCC GG^CGCC
NheI 2596 GCTAGC G^CTAGC
PmaCI 13 CACGTG CAC^GTG
PmlI 13 CACGTG CAC^GTG
SacI 256 GAGCTC GAGCT^C
SapI 1086 GAAGAGC GCTCTTC(1/4)
SfoI 2907 GGCGCC GGC^GCC
SmaI 440 CCCGGG CCC^GGG
SspBI 53 TGTACA T^GTACA
TaqI 63 TCGA T^CGA
Tru9I 680 TTAA T^TAA
TthHB8I 63 TCGA T^CGA
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 1520 GTCTAC GT^[AC][GT]AC
2271 GTCTAC
AcsI 138 GAATTT [GA]^AATT[CT]
3366 GAATTC
ApoI 138 GAATTT [GA]^AATT[CT]
3366 GAATTC
BamHI 3129 GGATCC G^GATCC
3492 GGATCC
BciVI 2846 GTATCC GTATCC(6/5)
3620 GTATCC
BglI 3347 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3538 GCCTACCAGGC
BlpI 2775 GCTGAGC GC^T[ACGT]AGC
3459 GCTCAGC
Bpu1102I 2775 GCTGAGC GC^T[ACGT]AGC
3459 GCTCAGC
BsaI 1273 GAGACC GGTCTC(1/5)
3728 GAGACC
BsaWI 1142 ACCGGA [AT]^CCGG[AT]
3770 TCCGGA
BsmBI 2388 CGTCTC CGTCTC(1/5)
3186 CGTCTC
CelII 2775 GCTGAGC GC^T[ACGT]AGC
3459 GCTCAGC
EarI 1086 GAAGAG CTCTTC(1/4)
2266 GAAGAG
HaeII 116 GGCGCT [GA]GCGC^[CT]
2907 GGCGCC
HincII 2517 GTTGAC GT[CT]^[GA]AC
3228 GTTGAC
HindII 2517 GTTGAC GT[CT]^[GA]AC
3228 GTTGAC
Ksp632I 1086 GAAGAG CTCTTC(1/4)
2266 GAAGAG
MaeII 14 ACGT A^CGT
2357 ACGT
NdeI 193 CATATG CA^TATG
1992 CATATG
SbfI 690 CCTGCAGG CCTGCA^GG
1659 CCTGCAGG
SexAI 299 ACCAGGT A^CC[AT]GGT
2526 ACCAGGT
Sse8387I 690 CCTGCAGG CCTGCA^GG
1659 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII