################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NarI NdeII NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1021 GTAGAC GT^[AC][GT]AC AccII 15 CGCG CG^CG AcsI 753 AAATTC [GA]^AATT[CT] AhdI 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 753 AAATTC [GA]^AATT[CT] AspEI 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 231 GGACC G^G[AT]CC BcnI 171 CCCGG CC^[GC]GG BpmI 205 CTCCAG CTGGAG(16/14) BsaAI 928 TACGTA [CT]AC^GT[GA] BsaI 1088 GGTCTC GGTCTC(1/5) BsmAI 1089 GTCTC GTCTC(1/5) BsrFI 4 GCCGGC [GA]^CCGG[CT] BstBI 962 TTCGAA TT^CGAA BstEII 718 GGTCACC G^GT[ACGT]ACC BstPI 718 GGTCACC G^GT[ACGT]ACC BstUI 15 CGCG CG^CG Cfr10I 4 GCCGGC [GA]^CCGG[CT] Cfr13I 231 GGACC G^G[ACGT]CC DsaI 789 CCATGG C^C[GA][CT]GG Eam1105I 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 760 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 718 GGTCACC G^GT[ACGT]ACC EcoT14I 789 CCATGG C^C[AT][AT]GG FauI 103 GCGGG CCCGC(4/6) MvnI 15 CGCG CG^CG MwoI 808 GCAGTGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 4 GCCGGC GCC^GGC NciI 171 CCCGG CC^[GC]GG NcoI 789 CCATGG C^CATGG NgoMIV 4 GCCGGC G^CCGGC NlaIV 160 GGTTCC GG[ACGT]^[ACGT]CC NspV 962 TTCGAA TT^CGAA PleI 277 GACTC GAGTC(4/5) PstI 291 CTGCAG CTGCA^G Sau96I 231 GGACC G^G[ACGT]CC SfcI 291 CTGCAG C^T[GA][CT]AG SfuI 962 TTCGAA TT^CGAA SnaBI 928 TACGTA TAC^GTA StuI 178 AGGCCT AGG^CCT StyI 789 CCATGG C^C[AT][AT]GG SwaI 1076 ATTTAAAT ATTT^AAAT TspRI 809 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BciVI 394 GGATAC GTATCC(6/5) 975 GGATAC BsaJI 170 CCCCGG C^C[ACGT][ACGT]GG 789 CCATGG BsmFI 107 GGGAC GGGAC(10/14) 1283 GTCCC Bsp1407I 825 TGTACA T^GTACA 922 TGTACA BspHI 371 TCATGA T^CATGA 419 TCATGA BsrDI 345 CATTGC GCAATG(2/0) 399 CATTGC BsrGI 825 TGTACA T^GTACA 922 TGTACA BsrI 228 ACTGG ACTGG(1/-1) 711 CCAGT BstF5I 48 GGATG GGATG(2/0) 96 GGATG Cac8I 4 GCCGGC GC[ACGT]^[ACGT]GC 474 GCTTGC DraI 66 TTTAAA TTT^AAA 1077 TTTAAA EaeI 2 TGGCCG [CT]^GGCC[GA] 149 TGGCCG FokI 48 GGATG GGATG(9/13) 96 GGATG HapII 5 CCGG C^CGG 172 CCGG HpaI 184 GTTAAC GTT^AAC 441 GTTAAC HpaII 5 CCGG C^CGG 172 CCGG MaeII 783 ACGT A^CGT 929 ACGT MboII 1054 GAAGA GAAGA(8/7) 1072 GAAGA MslI 1012 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1148 CATCTAAATG MspI 5 CCGG C^CGG 172 CCGG NspI 828 ACATGC [GA]CATG^[CT] 1205 GCATGT PvuII 259 CAGCTG CAG^CTG 665 CAGCTG RcaI 371 TCATGA T^CATGA 419 TCATGA SfaNI 52 GATGC GCATC(5/9) 427 GATGC SspBI 825 TGTACA T^GTACA 922 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BsiYI BslI BssKI BstNI CfoI DdeI EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MaeIII MnlI MseI MspA1I MvaI NdeI NlaIII RsaI ScrFI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I