Restriction map for
pFN21AE3630
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII BstAPI
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoRV
EcoT22I FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 492 GTATAC GT^[AC][GT]AC
Asp700I 1491 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1615 GTGCTC G[AT]GC[AT]^C
BbvCI 1246 GCTGAGG CCTCAGC(-5/-2)
BfaI 17 CTAG C^TAG
BglII 1091 AGATCT A^GATCT
BsaAI 1472 TACGTA [CT]AC^GT[GA]
BsaI 1081 GAGACC GGTCTC(1/5)
BsaWI 283 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1615 GTGCTC G[AT]GC[AT]^C
BsmBI 450 CGTCTC CGTCTC(1/5)
Bsp1407I 788 TGTACA T^GTACA
BspMI 693 GCAGGT ACCTGC(4/8)
BsrGI 788 TGTACA T^GTACA
BssSI 1338 CACGAG CACGAG(-5/-1)
Bst1107I 492 GTATAC GTA^TAC
BstBI 799 TTCGAA TT^CGAA
BstEII 162 GGTCACC G^GT[ACGT]ACC
BstPI 162 GGTCACC G^GT[ACGT]ACC
BstZ17I 492 GTATAC GTA^TAC
DraIII 165 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 432 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 427 CGGCCG C^GGCCG
EarI 1402 GAAGAG CTCTTC(1/4)
EclXI 427 CGGCCG C^GGCCG
Eco52I 427 CGGCCG C^GGCCG
EcoO65I 162 GGTCACC G^GT[ACGT]ACC
EcoRI 1006 GAATTC G^AATTC
HaeII 1533 AGCGCC [GA]GCGC^[CT]
HincII 808 GTCAAC GT[CT]^[GA]AC
HindII 808 GTCAAC GT[CT]^[GA]AC
Ksp632I 1402 GAAGAG CTCTTC(1/4)
MaeI 17 CTAG C^TAG
MspA1I 481 CAGCTG C[AC]G^C[GT]G
NcoI 1124 CCATGG C^CATGG
NspV 799 TTCGAA TT^CGAA
PflMI 652 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 481 CAGCTG CAG^CTG
ScaI 902 AGTACT AGT^ACT
SfuI 799 TTCGAA TT^CGAA
SnaBI 1472 TACGTA TAC^GTA
SspBI 788 TGTACA T^GTACA
StuI 1106 AGGCCT AGG^CCT
TfiI 486 GAATC G^A[AT]TC
Van91I 652 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1491 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 206 CGCG CG^CG
1023 CGCG
AcsI 1006 GAATTC [GA]^AATT[CT]
1222 GAATTT
ApaI 681 GGGCCC GGGCC^C
817 GGGCCC
ApoI 1006 GAATTC [GA]^AATT[CT]
1222 GAATTT
BalI 231 TGGCCA TGG^CCA
1155 TGGCCA
BcgI 1063 GCACCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1382 GCACGCTGGTCG
BclI 887 TGATCA T^GATCA
1265 TGATCA
BglI 420 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1054 GCCTTCATGGC
BsiEI 285 CGGTCG CG[GA][CT]^CG
427 CGGCCG
BsrBI 307 GAGCGG CCGCTC(-3/-3)
1177 GAGCGG
BsrDI 574 GCAATG GCAATG(2/0)
1001 GCAATG
BsrI 804 ACTGG ACTGG(1/-1)
1158 CCAGT
BstUI 206 CGCG CG^CG
1023 CGCG
BstXI 947 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1034 CCAACACCGTGG
BstYI 882 GGATCT [GA]^GATC[CT]
1091 AGATCT
FbaI 887 TGATCA T^GATCA
1265 TGATCA
HgaI 356 GACGC GACGC(5/10)
1021 GACGC
MflI 882 GGATCT [GA]^GATC[CT]
1091 AGATCT
MluNI 231 TGGCCA TGG^CCA
1155 TGGCCA
MscI 231 TGGCCA TGG^CCA
1155 TGGCCA
MvnI 206 CGCG CG^CG
1023 CGCG
NaeI 613 GCCGGC GCC^GGC
1508 GCCGGC
NgoMIV 613 GCCGGC G^CCGGC
1508 GCCGGC
SmlI 126 CTTGAG C^T[CT][GA]AG
718 CTCAAG
XhoII 882 GGATCT [GA]^GATC[CT]
1091 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I