Restriction map for
pFN21AE3648
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BsaAI BseAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 54 GACGTC GACGT^C
AgeI 278 ACCGGT A^CCGGT
AlwI 480 GATCC GGATC(4/5)
AlwNI 256 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 25 GTGCTC G[AT]GC[AT]^C
BanII 195 GGGCTC G[GA]GC[CT]^C
BbeI 115 GGCGCC GGCGC^C
BbsI 51 GAAGAC GAAGAC(2/6)
BglII 685 AGATCT A^GATCT
BlpI 523 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC
BpuAI 51 GAAGAC GAAGAC(2/6)
BsaBI 241 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 278 ACCGGT [AT]^CCGG[AT]
BseRI 355 GAGGAG GAGGAG(10/8)
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
BspMI 441 GCAGGT ACCTGC(4/8)
BsrBI 79 GAGCGG CCGCTC(-3/-3)
BsrI 659 CCAGT ACTGG(1/-1)
CelII 523 GCTGAGC GC^T[ACGT]AGC
DraII 361 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 643 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 186 CCACGG C^C[GA][CT]GG
EcoNI 348 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 361 GGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 406 GACGC GACGC(5/10)
HinfI 246 GAATC G^A[ACGT]TC
KasI 115 GGCGCC G^GCGCC
MaeIII 639 GTCAC ^GT[ACGT]AC
MamI 241 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 39 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 516 GCCGGC GCC^GGC
NarI 115 GGCGCC GG^CGCC
NgoMIV 516 GCCGGC G^CCGGC
NotI 217 GCGGCCGC GC^GGCCGC
PflMI 186 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 278 ACCGGT A^CCGGT
PstI 64 CTGCAG CTGCA^G
PvuII 448 CAGCTG CAG^CTG
SexAI 596 ACCTGGT A^CC[AT]GGT
SfcI 64 CTGCAG C^T[GA][CT]AG
SfoI 115 GGCGCC GGC^GCC
SmlI 484 CTCAAG C^T[CT][GA]AG
TfiI 246 GAATC G^A[AT]TC
Tsp45I 639 GTCAC ^GT[GC]AC
Van91I 186 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 54 GACGTC G[GA]^CG[CT]C
115 GGCGCC
AfaI 204 GTAC GT^AC
489 GTAC
AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
415 GACCGTGTC
BcnI 90 CCCGG CC^[GC]GG
210 CCGGG
BpmI 271 CTGGAG CTGGAG(16/14)
451 CTGGAG
BsaHI 54 GACGTC G[GA]^CG[CT]C
115 GGCGCC
BsaI 541 GAGACC GGTCTC(1/5)
583 GAGACC
BsgI 390 GTGCAG GTGCAG(16/14)
445 GTGCAG
BsiEI 218 CGGCCG CG[GA][CT]^CG
518 CGGCCG
BstAPI 308 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
507 GCAGGATGTGC
BstYI 479 AGATCC [GA]^GATC[CT]
685 AGATCT
EaeI 218 CGGCCG [CT]^GGCC[GA]
518 CGGCCG
EagI 218 CGGCCG C^GGCCG
518 CGGCCG
EarI 319 CTCTTC CTCTTC(1/4)
385 GAAGAG
EclXI 218 CGGCCG C^GGCCG
518 CGGCCG
Eco52I 218 CGGCCG C^GGCCG
518 CGGCCG
EcoT14I 365 CCTTGG C^C[AT][AT]GG
427 CCAAGG
FauI 174 GCGGG CCCGC(4/6)
370 GCGGG
Hin1I 54 GACGTC G[GA]^CG[CT]C
115 GGCGCC
HphI 233 TCACC GGTGA(8/7)
249 TCACC
Ksp632I 319 CTCTTC CTCTTC(1/4)
385 GAAGAG
MaeII 44 ACGT A^CGT
55 ACGT
MflI 479 AGATCC [GA]^GATC[CT]
685 AGATCT
NciI 90 CCCGG CC^[GC]GG
210 CCGGG
NlaIII 39 CATG CATG^
201 CATG
PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
415 GACCGTGTC
RsaI 204 GTAC GT^AC
489 GTAC
SfaNI 457 GATGC GCATC(5/9)
495 GATGC
StyI 365 CCTTGG C^C[AT][AT]GG
427 CCAAGG
Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
415 GACCGTGTC
XhoII 479 AGATCC [GA]^GATC[CT]
685 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI