Restriction map for
pFN21AE4390
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BbrPI
BciVI BclI BfrI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SalI SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Asp700I 1014 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 44 GAGCTC G[AT]GC[AT]^C
AvaII 642 GGTCC G^G[AT]CC
AvrII 55 CCTAGG C^CTAGG
BbeI 903 GGCGCC GGCGC^C
BbsI 682 GAAGAC GAAGAC(2/6)
BbvCI 459 CCTCAGC CCTCAGC(-5/-2)
BfaI 56 CTAG C^TAG
BglII 1166 AGATCT A^GATCT
BlnI 55 CCTAGG C^CTAGG
BpuAI 682 GAAGAC GAAGAC(2/6)
BsaAI 1114 TACGTG [CT]AC^GT[GA]
BsiEI 1067 CGACCG CG[GA][CT]^CG
BsiHKAI 44 GAGCTC G[AT]GC[AT]^C
BsmAI 106 GAGAC GTCTC(1/5)
BspHI 344 TCATGA T^CATGA
BsrBI 1135 GAGCGG CCGCTC(-3/-3)
BsrFI 1128 GCCGGC [GA]^CCGG[CT]
BstAPI 487 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 296 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1128 GCCGGC [GA]^CCGG[CT]
EarI 193 CTCTTC CTCTTC(1/4)
HaeII 903 GGCGCC [GA]GCGC^[CT]
HincII 352 GTCAAC GT[CT]^[GA]AC
HindII 352 GTCAAC GT[CT]^[GA]AC
KasI 903 GGCGCC G^GCGCC
Ksp632I 193 CTCTTC CTCTTC(1/4)
KspI 318 CCGCGG CCGC^GG
MaeI 56 CTAG C^TAG
NaeI 1128 GCCGGC GCC^GGC
NarI 903 GGCGCC GG^CGCC
NgoMIV 1128 GCCGGC G^CCGGC
NspI 130 ACATGC [GA]CATG^[CT]
RcaI 344 TCATGA T^CATGA
SacI 44 GAGCTC GAGCT^C
SacII 318 CCGCGG CCGC^GG
SfiI 1041 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 903 GGCGCC GGC^GCC
TfiI 885 GAATC G^A[AT]TC
Tsp509I 444 AATT ^AATT
XmnI 1014 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 903 GGCGCC G[GA]^CG[CT]C
1150 GACGCC
AlwI 1095 GATCC GGATC(4/5)
554 GGATC
ApaI 30 GGGCCC GGGCC^C
373 GGGCCC
BalI 239 TGGCCA TGG^CCA
1040 TGGCCA
BanI 903 GGCGCC G^G[CT][GA]CC
1177 GGCACC
BcgI 815 GCATCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
890 GCATGGCCTTCG
BsaHI 903 GGCGCC G[GA]^CG[CT]C
1150 GACGCC
BsaWI 334 ACCGGA [AT]^CCGG[AT]
1229 ACCGGA
BseRI 256 GAGGAG GAGGAG(10/8)
307 GAGGAG
BsmFI 137 GGGAC GGGAC(10/14)
643 GTCCC
BsrDI 165 CATTGC GCAATG(2/0)
843 CATTGC
BstYI 1094 AGATCC [GA]^GATC[CT]
1166 AGATCT
HgaI 1150 GACGC GACGC(5/10)
496 GCGTC
Hin1I 903 GGCGCC G[GA]^CG[CT]C
1150 GACGCC
MflI 1094 AGATCC [GA]^GATC[CT]
1166 AGATCT
MluNI 239 TGGCCA TGG^CCA
1040 TGGCCA
MscI 239 TGGCCA TGG^CCA
1040 TGGCCA
MslI 243 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
297 CATCGCCATG
PleI 523 GACTC GAGTC(4/5)
338 GAGTC
SmaI 20 CCCGGG CCC^GGG
509 CCCGGG
TaqI 278 TCGA T^CGA
860 TCGA
TthHB8I 278 TCGA T^CGA
860 TCGA
XhoII 1094 AGATCC [GA]^GATC[CT]
1166 AGATCT
XmaCI 20 CCCGGG C^CCGGG
509 CCCGGG
XmaI 20 CCCGGG C^CCGGG
509 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI Van91I XcmI