Restriction map for
pFN21AE4472
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CfoI
Cfr10I ClaI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 834 GTAC GT^AC
AflII 1194 CTTAAG C^TTAAG
AflIII 314 ACGTGT A^C[GA][CT]GT
AocI 1306 CCTGAGG CC^T[ACGT]AGG
AspHI 1444 GAGCAC G[AT]GC[AT]^C
BanI 61 GGCACC G^G[CT][GA]CC
BfrI 1194 CTTAAG C^TTAAG
BlpI 903 GCTCAGC GC^T[ACGT]AGC
BmyI 1444 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 903 GCTCAGC GC^T[ACGT]AGC
BsaI 940 GAGACC GGTCTC(1/5)
BsiHKAI 1444 GAGCAC G[AT]GC[AT]^C
BsmBI 917 GAGACG CGTCTC(1/5)
Bsp1286I 1444 GAGCAC G[AGT]GC[ACT]^C
BspHI 1346 TCATGA T^CATGA
BspMI 1448 ACCTGC ACCTGC(4/8)
BsrDI 38 GCAATG GCAATG(2/0)
BstAPI 80 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 806 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG
CelII 903 GCTCAGC GC^T[ACGT]AGC
CpoI 460 CGGACCG CG^G[AT]CCG
Eco81I 1306 CCTGAGG CC^T[ACGT]AGG
FauI 824 CCCGC CCCGC(4/6)
MseI 1195 TTAA T^TAA
NspI 827 GCATGC [GA]CATG^[CT]
PshAI 1155 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1346 TCATGA T^CATGA
RsaI 834 GTAC GT^AC
RsrII 460 CGGACCG CG^G[AT]CCG
SapI 1188 GCTCTTC GCTCTTC(1/4)
SphI 827 GCATGC GCATG^C
StuI 1166 AGGCCT AGG^CCT
Tru9I 1195 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 555 AAATTT [GA]^AATT[CT]
1090 AAATTC
ApoI 555 AAATTT [GA]^AATT[CT]
1090 AAATTC
AvaI 1324 CTCGAG C^[CT]CG[GA]G
1400 CTCGAG
BalI 207 TGGCCA TGG^CCA
869 TGGCCA
BbsI 325 GTCTTC GAAGAC(2/6)
856 GTCTTC
BclI 378 TGATCA T^GATCA
1286 TGATCA
BglII 596 AGATCT A^GATCT
1040 AGATCT
BpuAI 325 GTCTTC GAAGAC(2/6)
856 GTCTTC
BsoBI 1324 CTCGAG C^[CT]CG[GA]G
1400 CTCGAG
BssSI 70 CACGAG CACGAG(-5/-1)
124 CACGAG
BstYI 596 AGATCT [GA]^GATC[CT]
1040 AGATCT
DraII 334 GGGACCC [GA]G^G[ACGT]CC[CT]
779 AGGACCT
EcoO109I 334 GGGACCC [GA]G^G[ACGT]CC[CT]
779 AGGACCT
EcoRV 175 GATATC GAT^ATC
1147 GATATC
FbaI 378 TGATCA T^GATCA
1286 TGATCA
MaeII 314 ACGT A^CGT
614 ACGT
MflI 596 AGATCT [GA]^GATC[CT]
1040 AGATCT
MluNI 207 TGGCCA TGG^CCA
869 TGGCCA
MscI 207 TGGCCA TGG^CCA
869 TGGCCA
MslI 681 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1290 CACCGCTGTG
PaeR7I 1324 CTCGAG C^TCGAG
1400 CTCGAG
PflMI 356 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
861 CCACTCGCTGG
PpuMI 334 GGGACCC [GA]G^G[AT]CC[CT]
779 AGGACCT
PstI 1276 CTGCAG CTGCA^G
1420 CTGCAG
PvuII 433 CAGCTG CAG^CTG
544 CAGCTG
Van91I 356 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
861 CCACTCGCTGG
XcmI 210 CCAAGTCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
260 CCAGCAGCCAGGTGG
XhoI 1324 CTCGAG C^TCGAG
1400 CTCGAG
XhoII 596 AGATCT [GA]^GATC[CT]
1040 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I