Restriction map for
pFN21AE4401
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 236 CGCG CG^CG
BalI 906 TGGCCA TGG^CCA
BamHI 568 GGATCC G^GATCC
BbeI 328 GGCGCC GGCGC^C
BglII 866 AGATCT A^GATCT
BspMI 162 GCAGGT ACCTGC(4/8)
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BstEII 1239 GGTGACC G^GT[ACGT]ACC
BstPI 1239 GGTGACC G^GT[ACGT]ACC
BstUI 236 CGCG CG^CG
EcoO65I 1239 GGTGACC G^GT[ACGT]ACC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
HphI 1239 GGTGA GGTGA(8/7)
KasI 328 GGCGCC G^GCGCC
MluNI 906 TGGCCA TGG^CCA
MscI 906 TGGCCA TGG^CCA
MvnI 236 CGCG CG^CG
NaeI 841 GCCGGC GCC^GGC
NarI 328 GGCGCC GG^CGCC
NgoMIV 841 GCCGGC G^CCGGC
PaeR7I 935 CTCGAG C^TCGAG
PflMI 846 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 688 CTGCAG CTGCA^G
ScaI 899 AGTACT AGT^ACT
SfcI 688 CTGCAG C^T[GA][CT]AG
SfoI 328 GGCGCC GGC^GCC
SmlI 935 CTCGAG C^T[CT][GA]AG
StuI 671 AGGCCT AGG^CCT
StyI 47 CCAAGG C^C[AT][AT]GG
TaqI 936 TCGA T^CGA
TthHB8I 936 TCGA T^CGA
Van91I 846 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 41 CCAGACCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 935 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 712 GTCTAC GT^[AC][GT]AC
1165 GTCTAC
AlwNI 385 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
847 CAGGCCCTG
AocI 129 CCTCAGG CC^T[ACGT]AGG
285 CCTCAGG
AspHI 228 GTGCTC G[AT]GC[AT]^C
551 GAGCAC
AvaI 935 CTCGAG C^[CT]CG[GA]G
1024 CCCGAG
BanI 328 GGCGCC G^G[CT][GA]CC
658 GGTGCC
BsgI 160 GTGCAG GTGCAG(16/14)
700 GTGCAG
BsiHKAI 228 GTGCTC G[AT]GC[AT]^C
551 GAGCAC
BsoBI 935 CTCGAG C^[CT]CG[GA]G
1024 CCCGAG
BstXI 450 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1229 CCAGCGGCATGG
Bsu36I 129 CCTCAGG CC^T[ACGT]AGG
285 CCTCAGG
Eco81I 129 CCTCAGG CC^T[ACGT]AGG
285 CCTCAGG
FauI 67 GCGGG CCCGC(4/6)
325 GCGGG
HaeII 328 GGCGCC [GA]GCGC^[CT]
970 GGCGCT
MslI 402 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
556 CACCTCTGTG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI XhoII