Restriction map for
pFN21AE6348
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# NO CUT ENZYMES ( 124 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BseRI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfaNI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI
SwaI TaqI TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 288 GACGTC GACGT^C
AccI 499 GTCTAC GT^[AC][GT]AC
AlwNI 201 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 558 CCTCAGG CC^T[ACGT]AGG
AspI 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 314 CCCGAG C^[CT]CG[GA]G
AvaII 321 GGACC G^G[AT]CC
BamHI 485 GGATCC G^GATCC
BglI 751 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 455 AGATCT A^GATCT
BsaI 163 GAGACC GGTCTC(1/5)
BsgI 391 CTGCAC GTGCAG(16/14)
BsmBI 286 GAGACG CGTCTC(1/5)
BsoBI 314 CCCGAG C^[CT]CG[GA]G
BsrDI 732 CATTGC GCAATG(2/0)
BssHII 96 GCGCGC G^CGCGC
BstAPI 200 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 558 CCTCAGG CC^T[ACGT]AGG
DraIII 371 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 558 CCTCAGG CC^T[ACGT]AGG
EcoNI 554 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 18 GCGGG CCCGC(4/6)
HinfI 699 GAATC G^A[ACGT]TC
HphI 524 TCACC GGTGA(8/7)
MaeII 289 ACGT A^CGT
MseI 944 TTAA T^TAA
NaeI 924 GCCGGC GCC^GGC
NcoI 47 CCATGG C^CATGG
NdeI 337 CATATG CA^TATG
NgoMIV 924 GCCGGC G^CCGGC
PflFI 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 238 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 544 GAGCTC GAGCT^C
SapI 546 GCTCTTC GCTCTTC(1/4)
SbfI 753 CCTGCAGG CCTGCA^GG
SfiI 750 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 753 CCTGCAGG CCTGCA^GG
TfiI 699 GAATC G^A[AT]TC
Tru9I 944 TTAA T^TAA
Tsp509I 219 AATT ^AATT
Tth111I 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 238 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 486 GATCC GGATC(4/5)
485 GGATC
ApaI 20 GGGCCC GGGCC^C
913 GGGCCC
AspHI 544 GAGCTC G[AT]GC[AT]^C
715 GAGCAC
BsaWI 272 TCCGGT [AT]^CCGG[AT]
803 TCCGGT
BsiHKAI 544 GAGCTC G[AT]GC[AT]^C
715 GAGCAC
BspMI 332 ACCTGC ACCTGC(4/8)
531 GCAGGT
BsrFI 616 ACCGGC [GA]^CCGG[CT]
924 GCCGGC
BsrI 707 ACTGG ACTGG(1/-1)
156 CCAGT
BstXI 32 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
47 CCATGGAGATGG
BstYI 455 AGATCT [GA]^GATC[CT]
485 GGATCC
Cfr10I 616 ACCGGC [GA]^CCGG[CT]
924 GCCGGC
DdeI 559 CTCAG C^T[ACGT]AG
574 CTGAG
DpnII 456 GATC ^GATC
486 GATC
MaeIII 91 GTCAC ^GT[ACGT]AC
291 GTCAC
MboI 456 GATC ^GATC
486 GATC
MflI 455 AGATCT [GA]^GATC[CT]
485 GGATCC
MslI 48 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
354 CACCCCGGTG
NdeII 456 GATC ^GATC
486 GATC
NspI 509 GCATGT [GA]CATG^[CT]
863 GCATGT
Sau3AI 456 GATC ^GATC
486 GATC
Tsp45I 91 GTCAC ^GT[GC]AC
291 GTCAC
XhoII 455 AGATCT [GA]^GATC[CT]
485 GGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI Ksp632I MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PstI
PvuII RsaI Sau96I ScrFI SfcI
SfoI SspBI StyI TseI TspRI