Restriction map for
pFN21AE4567
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI
Bsu36I Cfr10I ClaI CpoI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MaeII MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1608 GTATAC GT^[AC][GT]AC
AlwNI 851 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 35 TGGCCA TGG^CCA
BciVI 892 GGATAC GTATCC(6/5)
BcnI 1131 CCGGG CC^[GC]GG
BglI 649 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 932 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 932 GCTCAGC GC^T[ACGT]AGC
BsaBI 1029 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 503 GAATGC GAATGC(1/-1)
BspHI 1185 TCATGA T^CATGA
BssHII 43 GCGCGC G^CGCGC
Bst1107I 1608 GTATAC GTA^TAC
BstYI 1546 GGATCT [GA]^GATC[CT]
BstZ17I 1608 GTATAC GTA^TAC
CelII 932 GCTCAGC GC^T[ACGT]AGC
DrdI 1105 GACAGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 35 TGGCCA [CT]^GGCC[GA]
EcoRI 1311 GAATTC G^AATTC
EcoT14I 2072 CCAAGG C^C[AT][AT]GG
HaeII 72 GGCGCT [GA]GCGC^[CT]
HapII 1131 CCGG C^CGG
HincII 529 GTTAAC GT[CT]^[GA]AC
HindII 529 GTTAAC GT[CT]^[GA]AC
HpaI 529 GTTAAC GTT^AAC
HpaII 1131 CCGG C^CGG
MamI 1029 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1546 GGATCT [GA]^GATC[CT]
MluNI 35 TGGCCA TGG^CCA
MscI 35 TGGCCA TGG^CCA
MslI 334 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1131 CCGG C^CGG
NciI 1131 CCGGG CC^[GC]GG
NdeI 1673 CATATG CA^TATG
PflMI 2072 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1185 TCATGA T^CATGA
SexAI 807 ACCAGGT A^CC[AT]GGT
SmlI 497 CTCAAG C^T[CT][GA]AG
SphI 1523 GCATGC GCATG^C
StuI 2075 AGGCCT AGG^CCT
StyI 2072 CCAAGG C^C[AT][AT]GG
Van91I 2072 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 943 CCAGATAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1546 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 1699 GATCC GGATC(4/5)
1546 GGATC
AseI 391 ATTAAT AT^TAAT
1348 ATTAAT
AsnI 391 ATTAAT AT^TAAT
1348 ATTAAT
AspHI 1601 GAGCAC G[AT]GC[AT]^C
1774 GTGCTC
AvaII 988 GGACC G^G[AT]CC
2064 GGACC
BsaI 794 GAGACC GGTCTC(1/5)
1230 GAGACC
BsgI 788 GTGCAG GTGCAG(16/14)
2122 GTGCAG
BsiHKAI 1601 GAGCAC G[AT]GC[AT]^C
1774 GTGCTC
BsmFI 569 GGGAC GGGAC(10/14)
1133 GGGAC
Bsp1407I 1396 TGTACA T^GTACA
1409 TGTACA
BsrGI 1396 TGTACA T^GTACA
1409 TGTACA
BsrI 1000 ACTGG ACTGG(1/-1)
294 CCAGT
BstEII 811 GGTTACC G^GT[ACGT]ACC
1126 GGTCACC
BstPI 811 GGTTACC G^GT[ACGT]ACC
1126 GGTCACC
BstXI 727 CCAGATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAACGACTTGG
DraI 556 TTTAAA TTT^AAA
1708 TTTAAA
EarI 112 CTCTTC CTCTTC(1/4)
2091 GAAGAG
EcoNI 439 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1450 CCTGATGAAGG
EcoO65I 811 GGTTACC G^GT[ACGT]ACC
1126 GGTCACC
FauI 51 CCCGC CCCGC(4/6)
85 GCGGG
Ksp632I 112 CTCTTC CTCTTC(1/4)
2091 GAAGAG
MfeI 582 CAATTG C^AATTG
887 CAATTG
MunI 582 CAATTG C^AATTG
887 CAATTG
NheI 327 GCTAGC G^CTAGC
1758 GCTAGC
NspI 1523 GCATGC [GA]CATG^[CT]
1971 GCATGT
PshBI 391 ATTAAT AT^TAAT
1348 ATTAAT
PstI 1901 CTGCAG CTGCA^G
2132 CTGCAG
SspBI 1396 TGTACA T^GTACA
1409 TGTACA
TaqI 1737 TCGA T^CGA
1791 TCGA
Tsp45I 1054 GTGAC ^GT[GC]AC
1127 GTCAC
TthHB8I 1737 TCGA T^CGA
1791 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI XmnI