Restriction map for
pFN21AE1358
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MboII MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI TfiI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 432 GCGG CCGC(-3/-1)
AfaI 255 GTAC GT^AC
AgeI 316 ACCGGT A^CCGGT
AspI 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 405 GGACC G^G[AT]CC
AvrII 360 CCTAGG C^CTAGG
BanI 364 GGTGCC G^G[CT][GA]CC
BcnI 5 CCGGG CC^[GC]GG
BfaI 361 CTAG C^TAG
BlnI 360 CCTAGG C^CTAGG
BsaBI 394 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 316 ACCGGT [AT]^CCGG[AT]
BsmAI 31 GTCTC GTCTC(1/5)
Bsp1407I 254 TGTACA T^GTACA
BsrFI 316 ACCGGT [GA]^CCGG[CT]
BsrGI 254 TGTACA T^GTACA
BsrI 121 ACTGG ACTGG(1/-1)
BstEII 125 GGTCACC G^GT[ACGT]ACC
BstNI 369 CCTGG CC^[AT]GG
BstPI 125 GGTCACC G^GT[ACGT]ACC
Cac8I 21 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 316 ACCGGT [GA]^CCGG[CT]
Cfr13I 405 GGACC G^G[ACGT]CC
EcoO65I 125 GGTCACC G^GT[ACGT]ACC
EcoRII 369 CCTGG ^CC[AT]GG
EcoT14I 360 CCTAGG C^C[AT][AT]GG
HincII 351 GTTGAC GT[CT]^[GA]AC
HindII 351 GTTGAC GT[CT]^[GA]AC
HinfI 330 GAGTC G^A[ACGT]TC
MaeI 361 CTAG C^TAG
MamI 394 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 91 TTAA T^TAA
MvaI 369 CCTGG CC^[AT]GG
NciI 5 CCGGG CC^[GC]GG
NlaIV 364 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 316 ACCGGT A^CCGGT
PleI 330 GAGTC GAGTC(4/5)
RsaI 255 GTAC GT^AC
Sau96I 405 GGACC G^G[ACGT]CC
SspBI 254 TGTACA T^GTACA
SspI 412 AATATT AAT^ATT
StyI 360 CCTAGG C^C[AT][AT]GG
TaqI 13 TCGA T^CGA
Tru9I 91 TTAA T^TAA
TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 13 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 297 AGCT AG^CT
385 AGCT
AlwI 15 GATCC GGATC(4/5)
311 GGATC
AlwNI 116 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
293 CAGAAGCTG
BbvI 25 GCTGC GCAGC(8/12)
298 GCTGC
BmyI 152 GGGCAC G[AGT]GC[ACT]^C
365 GTGCCC
Bsp1286I 152 GGGCAC G[AGT]GC[ACT]^C
365 GTGCCC
BssKI 5 CCGGG ^CC[ACGT]GG
369 CCTGG
BstF5I 203 GGATG GGATG(2/0)
393 GGATG
DdeI 328 CTGAG C^T[ACGT]AG
427 CTGAG
DpnII 15 GATC ^GATC
312 GATC
EaeI 2 TGGCCG [CT]^GGCC[GA]
264 TGGCCG
FokI 203 GGATG GGATG(9/13)
393 GGATG
HapII 5 CCGG C^CGG
317 CCGG
HpaII 5 CCGG C^CGG
317 CCGG
MboI 15 GATC ^GATC
312 GATC
MspI 5 CCGG C^CGG
317 CCGG
NdeII 15 GATC ^GATC
312 GATC
Sau3AI 15 GATC ^GATC
312 GATC
ScrFI 5 CCGGG CC^[ACGT]GG
369 CCTGG
TseI 25 GCTGC G^C[AT]GC
298 GCTGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
BsaJI Fnu4HI HaeIII HphI ItaI
MaeIII MnlI Tsp45I Tsp509I