Restriction map for
pFN21AE5225
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BspDI BspEI
BspMI BsrI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeI MaeII MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SalI SapI SbfI ScaI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I TspRI Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 652 GTAC GT^AC
AspHI 237 GAGCTC G[AT]GC[AT]^C
AvaII 453 GGTCC G^G[AT]CC
BbeI 43 GGCGCC GGCGC^C
BbsI 530 GAAGAC GAAGAC(2/6)
BbvCI 492 CCTCAGC CCTCAGC(-5/-2)
BpuAI 530 GAAGAC GAAGAC(2/6)
BsaBI 367 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 331 GAGACC GGTCTC(1/5)
BseRI 181 GAGGAG GAGGAG(10/8)
BsgI 51 GTGCAG GTGCAG(16/14)
BsiHKAI 237 GAGCTC G[AT]GC[AT]^C
BsmAI 331 GAGAC GTCTC(1/5)
Bsp1407I 651 TGTACA T^GTACA
BspHI 375 TCATGA T^CATGA
BsrDI 56 GCAATG GCAATG(2/0)
BsrGI 651 TGTACA T^GTACA
BssHII 137 GCGCGC G^CGCGC
BstAPI 264 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 231 CATCC GGATG(2/0)
EaeI 299 CGGCCA [CT]^GGCC[GA]
EarI 724 GAAGAG CTCTTC(1/4)
FokI 231 CATCC GGATG(9/13)
HgaI 279 GACGC GACGC(5/10)
KasI 43 GGCGCC G^GCGCC
Ksp632I 724 GAAGAG CTCTTC(1/4)
MaeIII 676 GTGAC ^GT[ACGT]AC
MamI 367 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 102 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NgoMIV 102 GCCGGC G^CCGGC
PflMI 439 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 375 TCATGA T^CATGA
RsaI 652 GTAC GT^AC
SacI 237 GAGCTC GAGCT^C
SfiI 92 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 43 GGCGCC GGC^GCC
SgrAI 23 CACCGGCG C[GA]^CCGG[CT]G
SmaI 88 CCCGGG CCC^GGG
SmlI 274 CTCAAG C^T[CT][GA]AG
SspBI 651 TGTACA T^GTACA
Tsp45I 676 GTGAC ^GT[GC]AC
Tsp509I 564 AATT ^AATT
Van91I 439 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 680 CCAGCACAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 88 CCCGGG C^CCGGG
XmaI 88 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 43 GGCGCC G[GA]^CG[CT]C
279 GACGCC
AflIII 407 ACATGT A^C[GA][CT]GT
629 ACATGT
AluI 194 AGCT AG^CT
238 AGCT
ApaI 9 GGGCCC GGGCC^C
91 GGGCCC
BamHI 75 GGATCC G^GATCC
708 GGATCC
BanI 21 GGCACC G^G[CT][GA]CC
43 GGCGCC
BglI 93 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
114 GCCCCCGCGGC
BpmI 285 CTGGAG CTGGAG(16/14)
599 CTGGAG
BsaHI 43 GGCGCC G[GA]^CG[CT]C
279 GACGCC
BspLU11I 407 ACATGT A^CATGT
629 ACATGT
BsrBI 70 GAGCGG CCGCTC(-3/-3)
166 GAGCGG
BsrFI 24 ACCGGC [GA]^CCGG[CT]
102 GCCGGC
BstYI 75 GGATCC [GA]^GATC[CT]
708 GGATCC
Cfr10I 24 ACCGGC [GA]^CCGG[CT]
102 GCCGGC
DraII 8 AGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCT
EcoO109I 8 AGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCT
EcoT14I 317 CCAAGG C^C[AT][AT]GG
486 CCTTGG
HaeII 43 GGCGCC [GA]GCGC^[CT]
548 AGCGCC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
279 GACGCC
HphI 387 GGTGA GGTGA(8/7)
314 TCACC
MflI 75 GGATCC [GA]^GATC[CT]
708 GGATCC
NspI 407 ACATGT [GA]CATG^[CT]
629 ACATGT
SfaNI 355 GATGC GCATC(5/9)
426 GCATC
SfcI 522 CTATAG C^T[GA][CT]AG
569 CTGTAG
StyI 317 CCAAGG C^C[AT][AT]GG
486 CCTTGG
XhoII 75 GGATCC [GA]^GATC[CT]
708 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AlwI AvaI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI KspI MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI SacII Sau3AI Sau96I
ScrFI TseI