Restriction map for
pFN21AE6126
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AluI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 278 GGTACC G^GTACC
AccII 14 CGCG CG^CG
AfaI 279 GTAC GT^AC
Alw44I 147 GTGCAC G^TGCAC
AlwI 198 GATCC GGATC(4/5)
ApaLI 147 GTGCAC G^TGCAC
Asp718I 278 GGTACC G^GTACC
BclI 62 TGATCA T^GATCA
BfaI 37 CTAG C^TAG
BglI 179 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 258 CTCCAG CTGGAG(16/14)
BsmAI 19 GTCTC GTCTC(1/5)
BsrBI 79 GAGCGG CCGCTC(-3/-3)
BsrI 90 CCAGT ACTGG(1/-1)
BstNI 260 CCAGG CC^[AT]GG
BstUI 14 CGCG CG^CG
BstYI 197 AGATCC [GA]^GATC[CT]
Cfr13I 71 GGGCC G^G[ACGT]CC
DraII 70 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 290 CCATGG C^C[GA][CT]GG
EcoNI 203 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 70 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 260 CCAGG ^CC[AT]GG
EcoT14I 290 CCATGG C^C[AT][AT]GG
FbaI 62 TGATCA T^GATCA
HaeIII 72 GGCC GG^CC
HinfI 17 GAGTC G^A[ACGT]TC
KpnI 278 GGTACC GGTAC^C
MaeI 37 CTAG C^TAG
MaeIII 40 GTAAC ^GT[ACGT]AC
MflI 197 AGATCC [GA]^GATC[CT]
MvaI 260 CCAGG CC^[AT]GG
MvnI 14 CGCG CG^CG
NcoI 290 CCATGG C^CATGG
PleI 17 GAGTC GAGTC(4/5)
RsaI 279 GTAC GT^AC
Sau96I 71 GGGCC G^G[ACGT]CC
StyI 290 CCATGG C^C[AT][AT]GG
TaqI 132 TCGA T^CGA
Tsp509I 310 AATT ^AATT
TspRI 91 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 132 TCGA T^CGA
XhoII 197 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 147 GTGCAC G[AT]GC[AT]^C
255 GTGCTC
BcnI 184 CCCGG CC^[GC]GG
267 CCCGG
BmyI 147 GTGCAC G[AGT]GC[ACT]^C
255 GTGCTC
BsaJI 183 CCCCGG C^C[ACGT][ACGT]GG
290 CCATGG
BseRI 31 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsiHKAI 147 GTGCAC G[AT]GC[AT]^C
255 GTGCTC
Bsp1286I 147 GTGCAC G[AGT]GC[ACT]^C
255 GTGCTC
BstF5I 84 GGATG GGATG(2/0)
143 GGATG
DpnII 63 GATC ^GATC
198 GATC
FokI 84 GGATG GGATG(9/13)
143 GGATG
HapII 185 CCGG C^CGG
268 CCGG
HpaII 185 CCGG C^CGG
268 CCGG
MboI 63 GATC ^GATC
198 GATC
MboII 300 GAAGA GAAGA(8/7)
206 TCTTC
MspA1I 154 CCGCTG C[AC]G^C[GT]G
282 CCGCTG
MspI 185 CCGG C^CGG
268 CCGG
NciI 184 CCCGG CC^[GC]GG
267 CCCGG
NdeII 63 GATC ^GATC
198 GATC
NlaIII 27 CATG CATG^
291 CATG
Sau3AI 63 GATC ^GATC
198 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BanI BbvI BsiYI BslI
BspMI BssKI Cac8I FauI Fnu4HI
ItaI MnlI MwoI NlaIV ScrFI
TseI