Restriction map for
pFN21AE6438
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MslI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 1006 GTAC GT^AC
AflII 1269 CTTAAG C^TTAAG
AlwI 1305 GATCC GGATC(4/5)
AlwNI 51 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1232 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 222 GAGCAC G[AT]GC[AT]^C
AvaII 404 GGTCC G^G[AT]CC
AviII 58 TGCGCA TGC^GCA
BalI 1071 TGGCCA TGG^CCA
BbvI 615 GCAGC GCAGC(8/12)
BciVI 1054 GTATCC GTATCC(6/5)
BfaI 398 CTAG C^TAG
BfrI 1269 CTTAAG C^TTAAG
BmyI 222 GAGCAC G[AGT]GC[ACT]^C
BsaAI 1348 TACGTA [CT]AC^GT[GA]
BsgI 1015 CTGCAC GTGCAG(16/14)
BsiHKAI 222 GAGCAC G[AT]GC[AT]^C
BsmAI 778 GTCTC GTCTC(1/5)
BsmFI 737 GGGAC GGGAC(10/14)
Bsp1286I 222 GAGCAC G[AGT]GC[ACT]^C
BsrDI 729 GCAATG GCAATG(2/0)
BstAPI 347 GCAGGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 59 GCGC GCG^C
DraI 1387 TTTAAA TTT^AAA
DsaI 864 CCACGG C^C[GA][CT]GG
EaeI 1071 TGGCCA [CT]^GGCC[GA]
EcoRI 573 GAATTC G^AATTC
EcoT14I 1216 CCAAGG C^C[AT][AT]GG
FspI 58 TGCGCA TGC^GCA
HhaI 59 GCGC GCG^C
HinP1I 59 GCGC G^CGC
HincII 558 GTTGAC GT[CT]^[GA]AC
HindII 558 GTTGAC GT[CT]^[GA]AC
MaeI 398 CTAG C^TAG
MfeI 526 CAATTG C^AATTG
MluNI 1071 TGGCCA TGG^CCA
MscI 1071 TGGCCA TGG^CCA
MspA1I 757 CAGCTG C[AC]G^C[GT]G
MunI 526 CAATTG C^AATTG
NlaIV 1173 GGCCCC GG[ACGT]^[ACGT]CC
PleI 319 GAGTC GAGTC(4/5)
PvuII 757 CAGCTG CAG^CTG
RsaI 1006 GTAC GT^AC
SfcI 1366 CTACAG C^T[GA][CT]AG
SnaBI 1348 TACGTA TAC^GTA
SspI 1234 AATATT AAT^ATT
StuI 870 AGGCCT AGG^CCT
StyI 1216 CCAAGG C^C[AT][AT]GG
TseI 615 GCAGC G^C[AT]GC
Tsp45I 53 GTCAC ^GT[GC]AC
XmnI 1232 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 7 CCGC CCGC(-3/-1)
1112 CCGC
BbsI 991 GAAGAC GAAGAC(2/6)
601 GTCTTC
BcnI 315 CCGGG CC^[GC]GG
1091 CCCGG
BglII 486 AGATCT A^GATCT
720 AGATCT
BpmI 1081 CTCCAG CTGGAG(16/14)
292 CTGGAG
BpuAI 991 GAAGAC GAAGAC(2/6)
601 GTCTTC
BsiYI 534 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCTGACCACGG
BslI 534 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCTGACCACGG
BsmI 343 GAATGC GAATGC(1/-1)
1274 GCATTC
BstF5I 543 GGATG GGATG(2/0)
567 GGATG
DraII 257 AGGCCCT [GA]G^G[ACGT]CC[CT]
1172 AGGCCCC
EarI 102 GAAGAG CTCTTC(1/4)
139 GAAGAG
EcoO109I 257 AGGCCCT [GA]G^G[ACGT]CC[CT]
1172 AGGCCCC
Fnu4HI 6 GCCGC GC^[ACGT]GC
615 GCAGC
FokI 543 GGATG GGATG(9/13)
567 GGATG
HapII 315 CCGG C^CGG
1092 CCGG
HpaII 315 CCGG C^CGG
1092 CCGG
ItaI 6 GCCGC GC^[ACGT]GC
615 GCAGC
Ksp632I 102 GAAGAG CTCTTC(1/4)
139 GAAGAG
MaeII 599 ACGT A^CGT
1349 ACGT
MaeIII 53 GTCAC ^GT[ACGT]AC
888 GTTAC
MspI 315 CCGG C^CGG
1092 CCGG
NciI 315 CCGGG CC^[GC]GG
1091 CCCGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BspHI
BspMI BsrI BssKI BstNI BstYI
Cfr13I DdeI DpnII EcoRII HaeIII
HinfI HphI MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII RcaI Sau3AI Sau96I ScrFI
SfaNI SmlI TfiI Tru9I Tsp509I
TspRI XhoII