Restriction map for
pFN21AE3592
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BspEI BspHI BspLU11I
BspMI BsrBI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
TspRI Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 393 GTAC GT^AC
AgeI 212 ACCGGT A^CCGGT
Alw44I 328 GTGCAC G^TGCAC
AlwNI 704 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 7 CCTAAGG CC^T[ACGT]AGG
ApaLI 328 GTGCAC G^TGCAC
AspHI 328 GTGCAC G[AT]GC[AT]^C
BanI 3 GGCACC G^G[CT][GA]CC
BcgI 456 CGACCTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 717 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 212 ACCGGT [AT]^CCGG[AT]
BsiHKAI 328 GTGCAC G[AT]GC[AT]^C
BsmFI 681 GGGAC GGGAC(10/14)
Bsp1407I 392 TGTACA T^GTACA
BspDI 580 ATCGAT AT^CGAT
BsrDI 301 GCAATG GCAATG(2/0)
BsrFI 212 ACCGGT [GA]^CCGG[CT]
BsrGI 392 TGTACA T^GTACA
BssSI 189 CACGAG CACGAG(-5/-1)
BstF5I 426 CATCC GGATG(2/0)
Bsu36I 7 CCTAAGG CC^T[ACGT]AGG
Cfr10I 212 ACCGGT [GA]^CCGG[CT]
ClaI 580 ATCGAT AT^CGAT
DraII 663 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 178 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 448 CTCTTC CTCTTC(1/4)
Eco81I 7 CCTAAGG CC^T[ACGT]AGG
EcoNI 643 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 663 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 363 GATATC GAT^ATC
EcoT14I 356 CCATGG C^C[AT][AT]GG
FauI 691 CCCGC CCCGC(4/6)
FokI 426 CATCC GGATG(9/13)
Ksp632I 448 CTCTTC CTCTTC(1/4)
MseI 223 TTAA T^TAA
MslI 351 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 356 CCATGG C^CATGG
NdeI 631 CATATG CA^TATG
PflMI 703 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 212 ACCGGT A^CCGGT
PpuMI 663 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 393 GTAC GT^AC
SmaI 58 CCCGGG CCC^GGG
SmlI 339 CTTGAG C^T[CT][GA]AG
SspBI 392 TGTACA T^GTACA
StyI 356 CCATGG C^C[AT][AT]GG
TaqI 581 TCGA T^CGA
Tru9I 223 TTAA T^TAA
Tsp509I 221 AATT ^AATT
TthHB8I 581 TCGA T^CGA
Van91I 703 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 173 CCAAGCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 515 GATCC GGATC(4/5)
546 GATCC
AvaI 58 CCCGGG C^[CT]CG[GA]G
70 CCCGAG
BbsI 76 GAAGAC GAAGAC(2/6)
590 GTCTTC
BcnI 58 CCCGG CC^[GC]GG
59 CCGGG
BpmI 647 CTCCAG CTGGAG(16/14)
563 CTGGAG
BpuAI 76 GAAGAC GAAGAC(2/6)
590 GTCTTC
BseRI 644 CTCCTC GAGGAG(10/8)
192 GAGGAG
BsgI 124 GTGCAG GTGCAG(16/14)
166 GTGCAG
BsoBI 58 CCCGGG C^[CT]CG[GA]G
70 CCCGAG
DdeI 8 CTAAG C^T[ACGT]AG
263 CTGAG
DsaI 356 CCATGG C^C[GA][CT]GG
488 CCGTGG
HapII 59 CCGG C^CGG
213 CCGG
HinfI 273 GATTC G^A[ACGT]TC
522 GAATC
HpaII 59 CCGG C^CGG
213 CCGG
HphI 323 TCACC GGTGA(8/7)
353 TCACC
MspI 59 CCGG C^CGG
213 CCGG
NciI 58 CCCGG CC^[GC]GG
59 CCGGG
NlaIII 357 CATG CATG^
519 CATG
TfiI 273 GATTC G^A[AT]TC
522 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I DpnII EcoRII
Fnu4HI HaeIII ItaI MboI MboII
MnlI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI