Restriction map for
pFN21AE1457
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AvrII BamHI BcgI BciVI
BclI BfrI BlnI BsaBI BsiWI
BspDI BspHI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoRV
EcoT22I FbaI FseI HpaI KpnI
MamI MfeI MluI MunI NcoI
NdeI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfuI SgrAI SpeI
SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 3610 GTCTAC GT^[AC][GT]AC
AccIII 280 TCCGGA T^CCGGA
AhdI 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 929 CCTCAGG CC^T[ACGT]AGG
Asp700I 3423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1703 TGCGCA TGC^GCA
BbeI 3148 GGCGCC GGCGC^C
BglII 1631 AGATCT A^GATCT
BsaI 2572 GGTCTC GGTCTC(1/5)
BseAI 280 TCCGGA T^CCGGA
BsmBI 3493 CGTCTC CGTCTC(1/5)
BsmI 2285 GAATGC GAATGC(1/-1)
BspEI 280 TCCGGA T^CCGGA
BspLU11I 3614 ACATGT A^CATGT
BssSI 535 CTCGTG CACGAG(-5/-1)
BstEII 1056 GGTGACC G^GT[ACGT]ACC
BstPI 1056 GGTGACC G^GT[ACGT]ACC
Bsu36I 929 CCTCAGG CC^T[ACGT]AGG
DrdI 2794 GACTGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 929 CCTCAGG CC^T[ACGT]AGG
EcoNI 141 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1056 GGTGACC G^GT[ACGT]ACC
FspI 1703 TGCGCA TGC^GCA
HindIII 1673 AAGCTT A^AGCTT
KasI 3148 GGCGCC G^GCGCC
MroI 280 TCCGGA T^CCGGA
MseI 2036 TTAA T^TAA
NaeI 3719 GCCGGC GCC^GGC
NarI 3148 GGCGCC GG^CGCC
NgoMIV 3719 GCCGGC G^CCGGC
NheI 1203 GCTAGC G^CTAGC
NotI 3711 GCGGCCGC GC^GGCCGC
PaeR7I 2467 CTCGAG C^TCGAG
PflMI 1431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 2092 GAGCTC GAGCT^C
SapI 435 GCTCTTC GCTCTTC(1/4)
SbfI 1770 CCTGCAGG CCTGCA^GG
SfoI 3148 GGCGCC GGC^GCC
SnaBI 1969 TACGTA TAC^GTA
SphI 561 GCATGC GCATG^C
Sse8387I 1770 CCTGCAGG CCTGCA^GG
StuI 3665 AGGCCT AGG^CCT
TfiI 1303 GATTC G^A[AT]TC
Tru9I 2036 TTAA T^TAA
Van91I 1431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2467 CTCGAG C^TCGAG
XmnI 3423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AcsI 276 GAATTC [GA]^AATT[CT]
897 GAATTC
AcyI 2596 GACGCC G[GA]^CG[CT]C
3148 GGCGCC
Alw44I 520 GTGCAC G^TGCAC
805 GTGCAC
AlwI 3174 GATCC GGATC(4/5)
684 GGATC
ApaLI 520 GTGCAC G^TGCAC
805 GTGCAC
ApoI 276 GAATTC [GA]^AATT[CT]
897 GAATTC
BalI 2775 TGGCCA TGG^CCA
3600 TGGCCA
BbrPI 808 CACGTG CAC^GTG
2146 CACGTG
BlpI 870 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
Bpu1102I 870 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
BsaHI 2596 GACGCC G[GA]^CG[CT]C
3148 GGCGCC
BsaWI 280 TCCGGA [AT]^CCGG[AT]
3500 TCCGGT
BsiEI 2364 CGGCCG CG[GA][CT]^CG
3712 CGGCCG
BsrBI 2047 GAGCGG CCGCTC(-3/-3)
3709 GAGCGG
BsrFI 2435 ACCGGC [GA]^CCGG[CT]
3719 GCCGGC
BssHII 243 GCGCGC G^CGCGC
2388 GCGCGC
CelII 870 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
Cfr10I 2435 ACCGGC [GA]^CCGG[CT]
3719 GCCGGC
EagI 2364 CGGCCG C^GGCCG
3712 CGGCCG
EclXI 2364 CGGCCG C^GGCCG
3712 CGGCCG
Eco52I 2364 CGGCCG C^GGCCG
3712 CGGCCG
EcoRI 276 GAATTC G^AATTC
897 GAATTC
Hin1I 2596 GACGCC G[GA]^CG[CT]C
3148 GGCGCC
HincII 1696 GTCAAC GT[CT]^[GA]AC
2523 GTTGAC
HindII 1696 GTCAAC GT[CT]^[GA]AC
2523 GTTGAC
KspI 358 CCGCGG CCGC^GG
2647 CCGCGG
MluNI 2775 TGGCCA TGG^CCA
3600 TGGCCA
MscI 2775 TGGCCA TGG^CCA
3600 TGGCCA
PmaCI 808 CACGTG CAC^GTG
2146 CACGTG
PmlI 808 CACGTG CAC^GTG
2146 CACGTG
SacII 358 CCGCGG CCGC^GG
2647 CCGCGG
SexAI 1052 ACCTGGT A^CC[AT]GGT
1124 ACCTGGT
SmaI 1397 CCCGGG CCC^GGG
2568 CCCGGG
XmaCI 1397 CCCGGG C^CCGGG
2568 CCCGGG
XmaI 1397 CCCGGG C^CCGGG
2568 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII