Restriction map for
pFN21AB7054
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbeI BclI BfrI BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI Tru9I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 1477 GTGCAC G^TGCAC
AocI 1117 CCTCAGG CC^T[ACGT]AGG
ApaLI 1477 GTGCAC G^TGCAC
Asp700I 1434 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1477 GTGCAC G[AT]GC[AT]^C
AspI 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 512 CCCGGG C^[CT]CG[GA]G
BamHI 936 GGATCC G^GATCC
BbrPI 1484 CACGTG CAC^GTG
BbvCI 974 CCTCAGC CCTCAGC(-5/-2)
BcgI 1290 GCAGTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 426 GGATAC GTATCC(6/5)
BglII 64 AGATCT A^GATCT
BlpI 1045 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1045 GCTGAGC GC^T[ACGT]AGC
BsaAI 1484 CACGTG [CT]AC^GT[GA]
BsaI 829 GAGACC GGTCTC(1/5)
BsiHKAI 1477 GTGCAC G[AT]GC[AT]^C
BsoBI 512 CCCGGG C^[CT]CG[GA]G
BspLU11I 1426 ACATGT A^CATGT
BspMI 1417 ACCTGC ACCTGC(4/8)
BsrBI 313 GAGCGG CCGCTC(-3/-3)
BstAPI 174 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1308 GGTGACC G^GT[ACGT]ACC
BstPI 1308 GGTGACC G^GT[ACGT]ACC
Bsu36I 1117 CCTCAGG CC^T[ACGT]AGG
CelII 1045 GCTGAGC GC^T[ACGT]AGC
DrdI 1095 GACTGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1117 CCTCAGG CC^T[ACGT]AGG
EcoNI 519 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1308 GGTGACC G^GT[ACGT]ACC
HincII 754 GTTGAC GT[CT]^[GA]AC
HindII 754 GTTGAC GT[CT]^[GA]AC
HindIII 1386 AAGCTT A^AGCTT
HinfI 912 GAGTC G^A[ACGT]TC
PflFI 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 912 GAGTC GAGTC(4/5)
PmaCI 1484 CACGTG CAC^GTG
PmlI 1484 CACGTG CAC^GTG
ScaI 592 AGTACT AGT^ACT
SmaI 512 CCCGGG CCC^GGG
StuI 47 AGGCCT AGG^CCT
Tth111I 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
XmnI 1434 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1426 ACATGT A^C[GA][CT]GT
1483 ACACGT
ApaI 125 GGGCCC GGGCC^C
515 GGGCCC
BalI 405 TGGCCA TGG^CCA
1580 TGGCCA
BanI 604 GGCACC G^G[CT][GA]CC
774 GGCACC
BanII 125 GGGCCC G[GA]GC[CT]^C
515 GGGCCC
BglI 259 GCCCATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
641 GCCACTGGGGC
DsaI 600 CCGTGG C^C[GA][CT]GG
1472 CCACGG
EaeI 405 TGGCCA [CT]^GGCC[GA]
1580 TGGCCA
EarI 852 CTCTTC CTCTTC(1/4)
1090 GAAGAG
HgaI 89 GACGC GACGC(5/10)
757 GACGC
Ksp632I 852 CTCTTC CTCTTC(1/4)
1090 GAAGAG
MaeII 985 ACGT A^CGT
1485 ACGT
MluNI 405 TGGCCA TGG^CCA
1580 TGGCCA
MscI 405 TGGCCA TGG^CCA
1580 TGGCCA
NspI 1426 ACATGT [GA]CATG^[CT]
1467 ACATGC
PflMI 249 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
370 CCAGCCTTTGG
PstI 181 CTGCAG CTGCA^G
282 CTGCAG
SfcI 181 CTGCAG C^T[GA][CT]AG
282 CTGCAG
TaqI 16 TCGA T^CGA
1299 TCGA
Tsp509I 320 AATT ^AATT
357 AATT
TthHB8I 16 TCGA T^CGA
1299 TCGA
Van91I 249 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
370 CCAGCCTTTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI XhoII