Restriction map for
pFN21AE6109
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI Ksp632I KspI MaeII
MamI MboI MboII MfeI MflI
MluI MluNI MscI MslI MunI
MvnI NaeI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TspRI
Tth111I Van91I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 423 TCCGGA T^CCGGA
AcyI 7 GGCGCC G[GA]^CG[CT]C
AfaI 311 GTAC GT^AC
BanI 7 GGCGCC G^G[CT][GA]CC
BanII 253 GAGCCC G[GA]GC[CT]^C
BbeI 7 GGCGCC GGCGC^C
BciVI 320 GTATCC GTATCC(6/5)
BmyI 253 GAGCCC G[AGT]GC[ACT]^C
BsaHI 7 GGCGCC G[GA]^CG[CT]C
BsaWI 423 TCCGGA [AT]^CCGG[AT]
BseAI 423 TCCGGA T^CCGGA
BseRI 148 GAGGAG GAGGAG(10/8)
BsgI 67 CTGCAC GTGCAG(16/14)
BsiYI 90 CCCCGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 90 CCCCGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 139 GAGAC GTCTC(1/5)
Bsp1286I 253 GAGCCC G[AGT]GC[ACT]^C
BspEI 423 TCCGGA T^CCGGA
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BstF5I 378 CATCC GGATG(2/0)
DdeI 388 CTTAG C^T[ACGT]AG
DraI 399 TTTAAA TTT^AAA
DraII 348 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 32 TGGCCG [CT]^GGCC[GA]
EcoO109I 348 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 126 CCATGG C^C[AT][AT]GG
FokI 378 CATCC GGATG(9/13)
HaeII 7 GGCGCC [GA]GCGC^[CT]
Hin1I 7 GGCGCC G[GA]^CG[CT]C
HinfI 413 GAATC G^A[ACGT]TC
KasI 7 GGCGCC G^GCGCC
MroI 423 TCCGGA T^CCGGA
MspA1I 21 CCGCTG C[AC]G^C[GT]G
NarI 7 GGCGCC GG^CGCC
NcoI 126 CCATGG C^CATGG
NspI 283 GCATGC [GA]CATG^[CT]
PaeR7I 56 CTCGAG C^TCGAG
PpuMI 348 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 311 GTAC GT^AC
SfoI 7 GGCGCC GGC^GCC
SmaI 44 CCCGGG CCC^GGG
SmlI 56 CTCGAG C^T[CT][GA]AG
SphI 283 GCATGC GCATG^C
StuI 60 AGGCCT AGG^CCT
StyI 126 CCATGG C^C[AT][AT]GG
TaqI 57 TCGA T^CGA
TfiI 413 GAATC G^A[AT]TC
Tsp45I 132 GTCAC ^GT[GC]AC
Tsp509I 206 AATT ^AATT
TthHB8I 57 TCGA T^CGA
XhoI 56 CTCGAG C^TCGAG
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AvaI 44 CCCGGG C^[CT]CG[GA]G
56 CTCGAG
AvaII 80 GGACC G^G[AT]CC
349 GGACC
BfaI 308 CTAG C^TAG
325 CTAG
BsoBI 44 CCCGGG C^[CT]CG[GA]G
56 CTCGAG
BstNI 200 CCTGG CC^[AT]GG
381 CCAGG
CfoI 8 GCGC GCG^C
26 GCGC
DsaI 95 CCGTGG C^C[GA][CT]GG
126 CCATGG
EcoRII 200 CCTGG ^CC[AT]GG
381 CCAGG
FauI 91 CCCGC CCCGC(4/6)
279 GCGGG
HhaI 8 GCGC GCG^C
26 GCGC
HinP1I 8 GCGC G^CGC
26 GCGC
MaeI 308 CTAG C^TAG
325 CTAG
MvaI 200 CCTGG CC^[AT]GG
381 CCAGG
MwoI 28 GCTGTGGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
274 GCTTTGCGGGC
NlaIII 127 CATG CATG^
284 CATG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BbvI BcnI BsaJI BsrI
BssKI Cac8I Cfr13I Fnu4HI HaeIII
HapII HpaII ItaI MaeIII MnlI
MseI MspI NciI NlaIV Sau96I
ScrFI SfaNI Tru9I TseI