Restriction map for
pFN21AE1392
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# NO CUT ENZYMES ( 214 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstF5I BstNI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeIII HapII HgaI
Hin1I HinfI HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI TseI Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AspHI 181 GAGCAC G[AT]GC[AT]^C
AvaII 223 GGACC G^G[AT]CC
BamHI 138 GGATCC G^GATCC
BanI 102 GGCACC G^G[CT][GA]CC
BpmI 256 CTGGAG CTGGAG(16/14)
BsiHKAI 181 GAGCAC G[AT]GC[AT]^C
BsiYI 186 CCTTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 186 CCTTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 222 GGGAC GGGAC(10/14)
BstEII 27 GGTGACC G^GT[ACGT]ACC
BstPI 27 GGTGACC G^GT[ACGT]ACC
BstYI 138 GGATCC [GA]^GATC[CT]
CfoI 262 GCGC GCG^C
Cfr13I 223 GGACC G^G[ACGT]CC
DdeI 199 CTAAG C^T[ACGT]AG
DpnII 139 GATC ^GATC
EcoNI 186 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 27 GGTGACC G^GT[ACGT]ACC
HaeII 261 GGCGCT [GA]GCGC^[CT]
HhaI 262 GCGC GCG^C
HinP1I 262 GCGC G^CGC
HincII 91 GTTGAC GT[CT]^[GA]AC
HindII 91 GTTGAC GT[CT]^[GA]AC
HindIII 148 AAGCTT A^AGCTT
HphI 27 GGTGA GGTGA(8/7)
MboI 139 GATC ^GATC
MflI 138 GGATCC [GA]^GATC[CT]
MseI 145 TTAA T^TAA
NdeII 139 GATC ^GATC
Sau3AI 139 GATC ^GATC
Sau96I 223 GGACC G^G[ACGT]CC
Tru9I 145 TTAA T^TAA
Tsp45I 28 GTGAC ^GT[GC]AC
XhoII 138 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 5 enzymes) #
###################################
AlwI 139 GATCC GGATC(4/5)
138 GGATC
BmyI 79 GTGCCC G[AGT]GC[ACT]^C
181 GAGCAC
Bsp1286I 79 GTGCCC G[AGT]GC[ACT]^C
181 GAGCAC
DraIII 184 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
271 CACTTGGTG
MwoI 232 GCTTCTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
255 GCTGGAGGCGC
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
AluI MaeIII MboII MnlI NlaIV