Restriction map for
pFN21AE5402
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 370 GTCTAC GT^[AC][GT]AC
AcsI 698 AAATTC [GA]^AATT[CT]
AocI 448 CCTCAGG CC^T[ACGT]AGG
Aor51HI 395 AGCGCT AGC^GCT
ApaI 592 GGGCCC GGGCC^C
ApoI 698 AAATTC [GA]^AATT[CT]
BanII 592 GGGCCC G[GA]GC[CT]^C
BciVI 585 GGATAC GTATCC(6/5)
BclI 140 TGATCA T^GATCA
BglII 306 AGATCT A^GATCT
BmyI 592 GGGCCC G[AGT]GC[ACT]^C
BsaAI 408 CACGTA [CT]AC^GT[GA]
BsgI 625 CTGCAC GTGCAG(16/14)
BsmAI 577 GTCTC GTCTC(1/5)
Bsp1286I 592 GGGCCC G[AGT]GC[ACT]^C
BsrBI 931 CCGCTC CCGCTC(-3/-3)
BsrI 456 ACTGG ACTGG(1/-1)
BstXI 705 CCATCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 448 CCTCAGG CC^T[ACGT]AGG
CfoI 396 GCGC GCG^C
DsaI 581 CCATGG C^C[GA][CT]GG
Eco47III 395 AGCGCT AGC^GCT
Eco81I 448 CCTCAGG CC^T[ACGT]AGG
FauI 350 CCCGC CCCGC(4/6)
FbaI 140 TGATCA T^GATCA
HaeII 395 AGCGCT [GA]GCGC^[CT]
HhaI 396 GCGC GCG^C
HinP1I 396 GCGC G^CGC
HindIII 619 AAGCTT A^AGCTT
MaeII 409 ACGT A^CGT
MspA1I 874 CAGCTG C[AC]G^C[GT]G
NcoI 581 CCATGG C^CATGG
PflMI 873 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 811 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 874 CAGCTG CAG^CTG
ScaI 215 AGTACT AGT^ACT
SmlI 317 CTCAAG C^T[CT][GA]AG
TaqI 153 TCGA T^CGA
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 153 TCGA T^CGA
Van91I 873 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 422 TCCGGA T^CCGGA
632 TCCGGA
AfaI 216 GTAC GT^AC
411 GTAC
AflIII 680 ACATGT A^C[GA][CT]GT
845 ACATGT
Asp700I 175 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
498 GAAGGTCTTC
BamHI 341 GGATCC G^GATCC
927 GGATCC
BbsI 891 GAAGAC GAAGAC(2/6)
502 GTCTTC
BcnI 186 CCCGG CC^[GC]GG
648 CCCGG
BpuAI 891 GAAGAC GAAGAC(2/6)
502 GTCTTC
BsaWI 422 TCCGGA [AT]^CCGG[AT]
632 TCCGGA
BseAI 422 TCCGGA T^CCGGA
632 TCCGGA
BspEI 422 TCCGGA T^CCGGA
632 TCCGGA
BspLU11I 680 ACATGT A^CATGT
845 ACATGT
BstAPI 604 GCAGGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
798 GCAGACCTTGC
Cac8I 604 GCAGGC GC[ACGT]^[ACGT]GC
872 GCCAGC
DdeI 402 CTCAG C^T[ACGT]AG
449 CTCAG
EcoNI 45 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
803 CCTTGCAGAGG
EcoT22I 461 ATGCAT ATGCA^T
784 ATGCAT
MroI 422 TCCGGA T^CCGGA
632 TCCGGA
MseI 73 TTAA T^TAA
262 TTAA
MslI 438 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
568 CATGTCAATG
NciI 186 CCCGG CC^[GC]GG
648 CCCGG
NsiI 461 ATGCAT ATGCA^T
784 ATGCAT
NspI 680 ACATGT [GA]CATG^[CT]
845 ACATGT
PleI 286 GACTC GAGTC(4/5)
190 GAGTC
RsaI 216 GTAC GT^AC
411 GTAC
Tru9I 73 TTAA T^TAA
262 TTAA
Tsp45I 530 GTGAC ^GT[GC]AC
686 GTCAC
Tsp509I 514 AATT ^AATT
699 AATT
XmnI 175 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
498 GAAGGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BpmI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstYI Cfr13I
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
TseI XhoII