Restriction map for
pFN21AE1437
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AgeI Alw44I Aor51HI ApaLI
AscI AspI AvrII BbeI BbsI
BcgI BciVI BglI BlnI BpuAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII
BstPI Cfr10I CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI HpaI KasI MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PstI PvuI PvuII
RsrII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 1171 GGTACC G^GTACC
AclI 2614 AACGTT AA^CGTT
AflII 1335 CTTAAG C^TTAAG
AflIII 1787 ACGTGT A^C[GA][CT]GT
AhdI 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1577 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 966 GGGCCC GGGCC^C
AseI 2206 ATTAAT AT^TAAT
AsnI 2206 ATTAAT AT^TAAT
Asp700I 420 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1171 GGTACC G^GTACC
AspEI 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 647 CTCGAG C^[CT]CG[GA]G
BamHI 1364 GGATCC G^GATCC
BbrPI 817 CACGTG CAC^GTG
BbvCI 1480 CCTCAGC CCTCAGC(-5/-2)
BclI 2126 TGATCA T^GATCA
BfrI 1335 CTTAAG C^TTAAG
BglII 2489 AGATCT A^GATCT
BsaAI 817 CACGTG [CT]AC^GT[GA]
BsoBI 647 CTCGAG C^[CT]CG[GA]G
BspDI 860 ATCGAT AT^CGAT
BspHI 2802 TCATGA T^CATGA
BspMI 1105 GCAGGT ACCTGC(4/8)
Bst1107I 1491 GTATAC GTA^TAC
BstXI 2594 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1491 GTATAC GTA^TAC
ClaI 860 ATCGAT AT^CGAT
DraIII 746 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1411 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1992 CCGCGG C^C[GA][CT]GG
Eam1105I 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2580 GAATTC G^AATTC
EcoT14I 587 CCAAGG C^C[AT][AT]GG
FbaI 2126 TGATCA T^GATCA
HaeII 1979 AGCGCC [GA]GCGC^[CT]
HincII 3026 GTCGAC GT[CT]^[GA]AC
HindII 3026 GTCGAC GT[CT]^[GA]AC
HindIII 125 AAGCTT A^AGCTT
KpnI 1171 GGTACC GGTAC^C
KspI 1992 CCGCGG CCGC^GG
MspA1I 1992 CCGCGG C[AC]G^C[GT]G
NspI 779 GCATGT [GA]CATG^[CT]
PaeR7I 647 CTCGAG C^TCGAG
PflMI 2278 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 817 CACGTG CAC^GTG
PmlI 817 CACGTG CAC^GTG
PshBI 2206 ATTAAT AT^TAAT
Psp1406I 2614 AACGTT AA^CGTT
RcaI 2802 TCATGA T^CATGA
SacII 1992 CCGCGG CCGC^GG
SalI 3026 GTCGAC G^TCGAC
SpeI 1275 ACTAGT A^CTAGT
SspI 848 AATATT AAT^ATT
StyI 587 CCAAGG C^C[AT][AT]GG
Van91I 2278 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1536 TCTAGA T^CTAGA
XhoI 647 CTCGAG C^TCGAG
XmnI 420 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1993 CGCG CG^CG
2231 CGCG
AccIII 2146 TCCGGA T^CCGGA
2948 TCCGGA
AcyI 973 GACGCC G[GA]^CG[CT]C
2213 GACGCC
AocI 1368 CCTAAGG CC^T[ACGT]AGG
2448 CCTGAGG
AviII 9 TGCGCA TGC^GCA
2852 TGCGCA
BalI 1008 TGGCCA TGG^CCA
2063 TGGCCA
BlpI 2508 GCTCAGC GC^T[ACGT]AGC
2740 GCTAAGC
Bpu1102I 2508 GCTCAGC GC^T[ACGT]AGC
2740 GCTAAGC
BsaHI 973 GACGCC G[GA]^CG[CT]C
2213 GACGCC
BsaI 280 GAGACC GGTCTC(1/5)
1766 GAGACC
BseAI 2146 TCCGGA T^CCGGA
2948 TCCGGA
BsgI 1236 CTGCAC GTGCAG(16/14)
2090 GTGCAG
BspEI 2146 TCCGGA T^CCGGA
2948 TCCGGA
BssSI 880 CACGAG CACGAG(-5/-1)
1292 CACGAG
BstUI 1993 CGCG CG^CG
2231 CGCG
Bsu36I 1368 CCTAAGG CC^T[ACGT]AGG
2448 CCTGAGG
CelII 2508 GCTCAGC GC^T[ACGT]AGC
2740 GCTAAGC
EarI 563 CTCTTC CTCTTC(1/4)
2359 CTCTTC
Eco81I 1368 CCTAAGG CC^T[ACGT]AGG
2448 CCTGAGG
EcoNI 1672 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1773 CCTGCAAGAGG
FauI 365 CCCGC CCCGC(4/6)
2229 CCCGC
FspI 9 TGCGCA TGC^GCA
2852 TGCGCA
Hin1I 973 GACGCC G[GA]^CG[CT]C
2213 GACGCC
Ksp632I 563 CTCTTC CTCTTC(1/4)
2359 CTCTTC
MluNI 1008 TGGCCA TGG^CCA
2063 TGGCCA
MroI 2146 TCCGGA T^CCGGA
2948 TCCGGA
MscI 1008 TGGCCA TGG^CCA
2063 TGGCCA
MvnI 1993 CGCG CG^CG
2231 CGCG
PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT]
1436 AGGTCCC
SacI 931 GAGCTC GAGCT^C
2313 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII