Restriction map for
pFN21AB7764
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BbrPI BbvCI BcgI BclI BfrI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AgeI 915 ACCGGT A^CCGGT
ApaI 92 GGGCCC GGGCC^C
AvrII 837 CCTAGG C^CTAGG
BbsI 637 GAAGAC GAAGAC(2/6)
BciVI 19 GGATAC GTATCC(6/5)
BglII 1043 AGATCT A^GATCT
BlnI 837 CCTAGG C^CTAGG
BpuAI 637 GAAGAC GAAGAC(2/6)
BsaWI 915 ACCGGT [AT]^CCGG[AT]
BsgI 301 GTGCAG GTGCAG(16/14)
BsmBI 965 CGTCTC CGTCTC(1/5)
BspDI 760 ATCGAT AT^CGAT
BspMI 1301 ACCTGC ACCTGC(4/8)
BstYI 1043 AGATCT [GA]^GATC[CT]
ClaI 760 ATCGAT AT^CGAT
EarI 643 GAAGAG CTCTTC(1/4)
HgaI 1468 GACGC GACGC(5/10)
Ksp632I 643 GAAGAG CTCTTC(1/4)
MaeII 152 ACGT A^CGT
MaeIII 1416 GTTAC ^GT[ACGT]AC
MflI 1043 AGATCT [GA]^GATC[CT]
NaeI 545 GCCGGC GCC^GGC
NgoMIV 545 GCCGGC G^CCGGC
NlaIII 483 CATG CATG^
PflMI 1040 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 915 ACCGGT A^CCGGT
SacI 694 GAGCTC GAGCT^C
SexAI 526 ACCTGGT A^CC[AT]GGT
SfaNI 306 GCATC GCATC(5/9)
SfiI 189 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 544 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 761 TCGA T^CGA
TthHB8I 761 TCGA T^CGA
Van91I 1040 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1043 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 168 GTAC GT^AC
366 GTAC
BalI 360 TGGCCA TGG^CCA
488 TGGCCA
BfaI 676 CTAG C^TAG
838 CTAG
BglI 190 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1443 GCCCAGGCGGC
BpmI 932 CTCCAG CTGGAG(16/14)
682 CTGGAG
BsaI 1112 GGTCTC GGTCTC(1/5)
1191 GGTCTC
EcoNI 510 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
552 CCTCAAGAAGG
HphI 497 TCACC GGTGA(8/7)
512 TCACC
MaeI 676 CTAG C^TAG
838 CTAG
MluNI 360 TGGCCA TGG^CCA
488 TGGCCA
MscI 360 TGGCCA TGG^CCA
488 TGGCCA
MslI 498 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
513 CACCCAGGTG
NotI 187 GCGGCCGC GC^GGCCGC
196 GCGGCCGC
PstI 1030 CTGCAG CTGCA^G
1147 CTGCAG
PvuII 13 CAGCTG CAG^CTG
262 CAGCTG
RsaI 168 GTAC GT^AC
366 GTAC
SmlI 553 CTCAAG C^T[CT][GA]AG
1420 CTCAAG
StuI 428 AGGCCT AGG^CCT
840 AGGCCT
TspRI 594 CACTG CA[GC]TG[ACGT][ACGT]^
947 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
KspI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV PleI PpuMI
SacII Sau3AI Sau96I ScrFI SfcI
SfoI SmaI StyI TseI XmaCI
XmaI