Restriction map for
pFN21AB7504
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FokI FseI FspI HgaI HincII
HindII HindIII HinfI HpaI HphI
KpnI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 13 GGCGCC G[GA]^CG[CT]C
AfaI 42 GTAC GT^AC
AflIII 38 ACATGT A^C[GA][CT]GT
Aor51HI 359 AGCGCT AGC^GCT
AvaII 234 GGACC G^G[AT]CC
BanI 13 GGCGCC G^G[CT][GA]CC
BbeI 13 GGCGCC GGCGC^C
BclI 32 TGATCA T^GATCA
BpmI 160 CTGGAG CTGGAG(16/14)
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsaI 67 GGTCTC GGTCTC(1/5)
BsmBI 62 GAGACG CGTCTC(1/5)
BsmFI 641 GTCCC GGGAC(10/14)
BspHI 338 TCATGA T^CATGA
BspLU11I 38 ACATGT A^CATGT
BspMI 44 ACCTGC ACCTGC(4/8)
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BssHII 473 GCGCGC G^CGCGC
EarI 70 CTCTTC CTCTTC(1/4)
Eco47III 359 AGCGCT AGC^GCT
EcoNI 390 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 32 TGATCA T^GATCA
Hin1I 13 GGCGCC G[GA]^CG[CT]C
KasI 13 GGCGCC G^GCGCC
Ksp632I 70 CTCTTC CTCTTC(1/4)
MaeIII 212 GTCAC ^GT[ACGT]AC
MslI 421 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 13 GGCGCC GG^CGCC
NotI 457 GCGGCCGC GC^GGCCGC
NspI 38 ACATGT [GA]CATG^[CT]
PflMI 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 338 TCATGA T^CATGA
RsaI 42 GTAC GT^AC
SfaNI 194 GCATC GCATC(5/9)
SfiI 543 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 13 GGCGCC GGC^GCC
StuI 398 AGGCCT AGG^CCT
TaqI 377 TCGA T^CGA
Tsp45I 212 GTCAC ^GT[GC]AC
TthHB8I 377 TCGA T^CGA
Van91I 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 52 GATCC GGATC(4/5)
138 GATCC
BglI 451 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
544 GCCGCCTCGGC
BlpI 105 GCTGAGC GC^T[ACGT]AGC
300 GCTGAGC
Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC
300 GCTGAGC
BsiEI 458 CGGCCG CG[GA][CT]^CG
551 CGGCCG
BsrI 581 ACTGG ACTGG(1/-1)
638 CCAGT
BstAPI 150 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
439 GCAGCTGCTGC
BstYI 51 AGATCC [GA]^GATC[CT]
137 AGATCC
CelII 105 GCTGAGC GC^T[ACGT]AGC
300 GCTGAGC
DdeI 106 CTGAG C^T[ACGT]AG
301 CTGAG
EagI 458 CGGCCG C^GGCCG
551 CGGCCG
EclXI 458 CGGCCG C^GGCCG
551 CGGCCG
Eco52I 458 CGGCCG C^GGCCG
551 CGGCCG
HaeII 13 GGCGCC [GA]GCGC^[CT]
359 AGCGCT
KspI 113 CCGCGG CCGC^GG
455 CCGCGG
MboII 84 GAAGA GAAGA(8/7)
71 TCTTC
MflI 51 AGATCC [GA]^GATC[CT]
137 AGATCC
PvuII 142 CAGCTG CAG^CTG
440 CAGCTG
SacII 113 CCGCGG CCGC^GG
455 CCGCGG
SmaI 591 CCCGGG CCC^GGG
661 CCCGGG
XhoII 51 AGATCC [GA]^GATC[CT]
137 AGATCC
XmaCI 591 CCCGGG C^CCGGG
661 CCCGGG
XmaI 591 CCCGGG C^CCGGG
661 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AvaI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI