Restriction map for
pFN21AE4491
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FspI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1435 GAATTC [GA]^AATT[CT]
AcyI 404 GGCGCC G[GA]^CG[CT]C
AfaI 1015 GTAC GT^AC
AhdI 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 91 CCTCAGG CC^T[ACGT]AGG
ApoI 1435 GAATTC [GA]^AATT[CT]
AspEI 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 404 GGCGCC G^G[CT][GA]CC
BbeI 404 GGCGCC GGCGC^C
BbvCI 499 GCTGAGG CCTCAGC(-5/-2)
BsaHI 404 GGCGCC G[GA]^CG[CT]C
BsmI 744 GAATGC GAATGC(1/-1)
BspMI 1055 GCAGGT ACCTGC(4/8)
BsrBI 258 GAGCGG CCGCTC(-3/-3)
Bsu36I 91 CCTCAGG CC^T[ACGT]AGG
Eam1105I 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 91 CCTCAGG CC^T[ACGT]AGG
EcoRI 1435 GAATTC G^AATTC
FseI 313 GGCCGGCC GGCCGG^CC
Hin1I 404 GGCGCC G[GA]^CG[CT]C
KasI 404 GGCGCC G^GCGCC
MslI 240 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 1065 GCGGCCCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 404 GGCGCC GG^CGCC
NcoI 127 CCATGG C^CATGG
PflFI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 373 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 376 GACTC GAGTC(4/5)
PshAI 582 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 220 CTGCAG CTGCA^G
RsaI 1015 GTAC GT^AC
SapI 478 GAAGAGC GCTCTTC(1/4)
SfoI 404 GGCGCC GGC^GCC
SgrAI 400 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 84 CTCAAG C^T[CT][GA]AG
SphI 1497 GCATGC GCATG^C
TaqI 1316 TCGA T^CGA
TfiI 114 GAATC G^A[AT]TC
Tth111I 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1316 TCGA T^CGA
Van91I 373 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BcnI 834 CCGGG CC^[GC]GG
1070 CCCGG
BfaI 649 CTAG C^TAG
1039 CTAG
BlpI 255 GCTGAGC GC^T[ACGT]AGC
642 GCTTAGC
BpmI 981 CTGGAG CTGGAG(16/14)
1117 CTGGAG
Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC
642 GCTTAGC
BsaI 1120 GAGACC GGTCTC(1/5)
1378 GAGACC
BseRI 456 CTCCTC GAGGAG(10/8)
1033 GAGGAG
BsrDI 438 CATTGC GCAATG(2/0)
1383 CATTGC
CelII 255 GCTGAGC GC^T[ACGT]AGC
642 GCTTAGC
EaeI 23 CGGCCA [CT]^GGCC[GA]
609 TGGCCG
EcoT14I 127 CCATGG C^C[AT][AT]GG
1205 CCAAGG
HaeII 404 GGCGCC [GA]GCGC^[CT]
614 GGCGCT
HincII 652 GTCAAC GT[CT]^[GA]AC
1264 GTCAAC
HindII 652 GTCAAC GT[CT]^[GA]AC
1264 GTCAAC
HinfI 114 GAATC G^A[ACGT]TC
376 GACTC
MaeI 649 CTAG C^TAG
1039 CTAG
MaeII 215 ACGT A^CGT
517 ACGT
NciI 834 CCGGG CC^[GC]GG
1070 CCCGG
StuI 105 AGGCCT AGG^CCT
1106 AGGCCT
StyI 127 CCATGG C^C[AT][AT]GG
1205 CCAAGG
XcmI 118 CCACCAGGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
239 CCACACCCGTGGTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AlwNI Asp700I
AspHI AvaII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI NaeI
NdeII NgoMIV NlaIII NlaIV NspI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI