Restriction map for
pFN21AE5108
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeII
MaeIII MamI MboII MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 297 CGCG CG^CG
AcsI 87 GAATTC [GA]^AATT[CT]
Alw44I 156 GTGCAC G^TGCAC
AlwI 279 GATCC GGATC(4/5)
Aor51HI 400 AGCGCT AGC^GCT
ApaLI 156 GTGCAC G^TGCAC
ApoI 87 GAATTC [GA]^AATT[CT]
Asp700I 342 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 378 GGCACC G^G[CT][GA]CC
BlpI 189 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 189 GCTGAGC GC^T[ACGT]AGC
BsgI 307 GTGCAG GTGCAG(16/14)
BspMI 323 ACCTGC ACCTGC(4/8)
BstUI 297 CGCG CG^CG
BstYI 278 AGATCC [GA]^GATC[CT]
CelII 189 GCTGAGC GC^T[ACGT]AGC
DdeI 190 CTGAG C^T[ACGT]AG
DpnII 279 GATC ^GATC
DrdI 204 GACCTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 390 CCACGG C^C[GA][CT]GG
Eco47III 400 AGCGCT AGC^GCT
EcoRI 87 GAATTC G^AATTC
HaeII 400 AGCGCT [GA]GCGC^[CT]
HinfI 115 GAATC G^A[ACGT]TC
MboI 279 GATC ^GATC
MflI 278 AGATCC [GA]^GATC[CT]
MvnI 297 CGCG CG^CG
NdeII 279 GATC ^GATC
PvuII 173 CAGCTG CAG^CTG
SacI 228 GAGCTC GAGCT^C
Sau3AI 279 GATC ^GATC
SmaI 136 CCCGGG CCC^GGG
TfiI 115 GAATC G^A[AT]TC
Tsp509I 88 AATT ^AATT
TspRI 8 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 91 TCTAGA T^CTAGA
XhoII 278 AGATCC [GA]^GATC[CT]
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
XmnI 342 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 22 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
145 CAGAGCCTG
AspHI 156 GTGCAC G[AT]GC[AT]^C
228 GAGCTC
AvaI 136 CCCGGG C^[CT]CG[GA]G
223 CCCGAG
AvaII 203 GGACC G^G[AT]CC
321 GGACC
BanII 228 GAGCTC G[GA]GC[CT]^C
237 GAGCCC
BfaI 92 CTAG C^TAG
112 CTAG
BsiHKAI 156 GTGCAC G[AT]GC[AT]^C
228 GAGCTC
BsmFI 202 GGGAC GGGAC(10/14)
213 GTCCC
BsoBI 136 CCCGGG C^[CT]CG[GA]G
223 CCCGAG
BsrI 9 ACTGG ACTGG(1/-1)
338 ACTGG
BstAPI 21 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
144 GCAGAGCCTGC
DraII 202 GGGACCT [GA]G^G[ACGT]CC[CT]
320 AGGACCT
EcoO109I 202 GGGACCT [GA]G^G[ACGT]CC[CT]
320 AGGACCT
MaeI 92 CTAG C^TAG
112 CTAG
MspA1I 173 CAGCTG C[AC]G^C[GT]G
310 CAGCGG
PpuMI 202 GGGACCT [GA]G^G[AT]CC[CT]
320 AGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI