Restriction map for
pFN21AE0957
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MluI MroI MslI MvnI
NaeI NarI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 710 AAATTT [GA]^AATT[CT]
Aor51HI 21 AGCGCT AGC^GCT
ApoI 710 AAATTT [GA]^AATT[CT]
Asp700I 663 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5 CCCGGG C^[CT]CG[GA]G
AvaII 328 GGACC G^G[AT]CC
BalI 341 TGGCCA TGG^CCA
BanI 1263 GGTGCC G^G[CT][GA]CC
BciVI 1328 GTATCC GTATCC(6/5)
BfaI 899 CTAG C^TAG
BglII 504 AGATCT A^GATCT
BlpI 17 GCTCAGC GC^T[ACGT]AGC
BpmI 675 CTCCAG CTGGAG(16/14)
Bpu1102I 17 GCTCAGC GC^T[ACGT]AGC
BsaI 441 GAGACC GGTCTC(1/5)
BsgI 289 CTGCAC GTGCAG(16/14)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsoBI 5 CCCGGG C^[CT]CG[GA]G
BspHI 816 TCATGA T^CATGA
BsrBI 1406 GAGCGG CCGCTC(-3/-3)
BsrDI 756 CATTGC GCAATG(2/0)
BstF5I 744 CATCC GGATG(2/0)
CelII 17 GCTCAGC GC^T[ACGT]AGC
EagI 53 CGGCCG C^GGCCG
EarI 1424 CTCTTC CTCTTC(1/4)
EclXI 53 CGGCCG C^GGCCG
Eco47III 21 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
FauI 1412 CCCGC CCCGC(4/6)
FokI 744 CATCC GGATG(9/13)
HincII 413 GTCGAC GT[CT]^[GA]AC
HindII 413 GTCGAC GT[CT]^[GA]AC
Ksp632I 1424 CTCTTC CTCTTC(1/4)
MaeI 899 CTAG C^TAG
MluNI 341 TGGCCA TGG^CCA
MscI 341 TGGCCA TGG^CCA
MseI 1433 TTAA T^TAA
MspA1I 50 CAGCGG C[AC]G^C[GT]G
NdeI 919 CATATG CA^TATG
NotI 52 GCGGCCGC GC^GGCCGC
PflMI 319 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 816 TCATGA T^CATGA
SacI 61 GAGCTC GAGCT^C
SalI 413 GTCGAC G^TCGAC
SapI 1423 GCTCTTC GCTCTTC(1/4)
SfcI 426 CTACAG C^T[GA][CT]AG
SmaI 5 CCCGGG CCC^GGG
SmlI 212 CTCAAG C^T[CT][GA]AG
SphI 403 GCATGC GCATG^C
SspI 666 AATATT AAT^ATT
Tru9I 1433 TTAA T^TAA
Van91I 319 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1094 CCAAGAGGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
XmnI 663 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 413 GTCGAC GT^[AC][GT]AC
545 GTCTAC
AfaI 935 GTAC GT^AC
1239 GTAC
AlwI 1273 GATCC GGATC(4/5)
532 GGATC
AspHI 61 GAGCTC G[AT]GC[AT]^C
420 GTGCTC
BanII 61 GAGCTC G[GA]GC[CT]^C
825 GGGCTC
BclI 176 TGATCA T^GATCA
875 TGATCA
BseRI 672 CTCCTC GAGGAG(10/8)
1234 GAGGAG
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
420 GTGCTC
BsmI 207 GAATGC GAATGC(1/-1)
253 GAATGC
BsrI 1193 ACTGG ACTGG(1/-1)
1338 CCAGT
BstXI 332 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1333 CCAAACCAGTGG
Cfr13I 328 GGACC G^G[ACGT]CC
1410 GGCCC
DsaI 332 CCATGG C^C[GA][CT]GG
863 CCATGG
EaeI 53 CGGCCG [CT]^GGCC[GA]
341 TGGCCA
FbaI 176 TGATCA T^GATCA
875 TGATCA
HapII 6 CCGG C^CGG
1206 CCGG
HpaII 6 CCGG C^CGG
1206 CCGG
MspI 6 CCGG C^CGG
1206 CCGG
NcoI 332 CCATGG C^CATGG
863 CCATGG
NspI 133 GCATGT [GA]CATG^[CT]
403 GCATGC
RsaI 935 GTAC GT^AC
1239 GTAC
Sau96I 328 GGACC G^G[ACGT]CC
1410 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstNI
BstYI Cac8I CfoI DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MboII MfeI MflI
MnlI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII