Restriction map for
pFN21AE9533
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BcgI BclI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrI BssHII
Bst1107I BstBI BstF5I BstZ17I Bsu36I
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluNI MroI MscI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfaNI SfuI SgrAI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 977 ACGCGT A^C[GA][CT]GT
AlwNI 415 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 395 AGCGCT AGC^GCT
BanI 197 GGCGCC G^G[CT][GA]CC
BbeI 197 GGCGCC GGCGC^C
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
BciVI 1188 GGATAC GTATCC(6/5)
BfaI 403 CTAG C^TAG
BglI 21 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 781 GGTCTC GGTCTC(1/5)
BsmBI 541 GAGACG CGTCTC(1/5)
BsmFI 1175 GTCCC GGGAC(10/14)
Bsp1407I 995 TGTACA T^GTACA
BspMI 695 ACCTGC ACCTGC(4/8)
BsrBI 191 GAGCGG CCGCTC(-3/-3)
BsrDI 146 GCAATG GCAATG(2/0)
BsrFI 261 GCCGGT [GA]^CCGG[CT]
BsrGI 995 TGTACA T^GTACA
Cfr10I 261 GCCGGT [GA]^CCGG[CT]
EaeI 509 TGGCCG [CT]^GGCC[GA]
Eco47III 395 AGCGCT AGC^GCT
KasI 197 GGCGCC G^GCGCC
MaeI 403 CTAG C^TAG
MluI 977 ACGCGT A^CGCGT
NarI 197 GGCGCC GG^CGCC
NcoI 506 CCATGG C^CATGG
NruI 571 TCGCGA TCG^CGA
PaeR7I 538 CTCGAG C^TCGAG
PflMI 693 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1052 CAGCTG CAG^CTG
SacI 268 GAGCTC GAGCT^C
SfcI 273 CTACAG C^T[GA][CT]AG
SfiI 20 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 197 GGCGCC GGC^GCC
SmlI 538 CTCGAG C^T[CT][GA]AG
SnaBI 964 TACGTA TAC^GTA
SspBI 995 TGTACA T^GTACA
StuI 381 AGGCCT AGG^CCT
TaqI 539 TCGA T^CGA
Tsp509I 1000 AATT ^AATT
TthHB8I 539 TCGA T^CGA
Van91I 693 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 538 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 452 GGACC G^G[AT]CC
1226 GGACC
BamHI 863 GGATCC G^GATCC
1209 GGATCC
BanII 268 GAGCTC G[GA]GC[CT]^C
562 GAGCCC
BbrPI 163 CACGTG CAC^GTG
211 CACGTG
BpmI 874 CTCCAG CTGGAG(16/14)
760 CTGGAG
BssSI 343 CACGAG CACGAG(-5/-1)
474 CTCGTG
BstAPI 162 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
658 GCACTAACTGC
BstEII 1023 GGTGACC G^GT[ACGT]ACC
1154 GGTTACC
BstPI 1023 GGTGACC G^GT[ACGT]ACC
1154 GGTTACC
BstYI 863 GGATCC [GA]^GATC[CT]
1209 GGATCC
DpnII 864 GATC ^GATC
1210 GATC
DraIII 163 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
208 CACCACGTG
EarI 1064 CTCTTC CTCTTC(1/4)
1102 CTCTTC
EcoO65I 1023 GGTGACC G^GT[ACGT]ACC
1154 GGTTACC
Ksp632I 1064 CTCTTC CTCTTC(1/4)
1102 CTCTTC
MboI 864 GATC ^GATC
1210 GATC
MflI 863 GGATCC [GA]^GATC[CT]
1209 GGATCC
MslI 501 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1179 CACCCTAATG
NdeII 864 GATC ^GATC
1210 GATC
PmaCI 163 CACGTG CAC^GTG
211 CACGTG
PmlI 163 CACGTG CAC^GTG
211 CACGTG
Sau3AI 864 GATC ^GATC
1210 GATC
SmaI 42 CCCGGG CCC^GGG
735 CCCGGG
TspRI 686 CAGTG CA[GC]TG[ACGT][ACGT]^
992 CACTG
XhoII 863 GGATCC [GA]^GATC[CT]
1209 GGATCC
XmaCI 42 CCCGGG C^CCGGG
735 CCCGGG
XmaI 42 CCCGGG C^CCGGG
735 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaLI AspHI AvaI
BbsI BbvI BcnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BssKI BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DsaI EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI RsaI SacII Sau96I ScrFI
StyI TseI Tsp45I