Restriction map for
pFN21AE4520
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AciI AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BciVI BglI BlnI BmyI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I BspDI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FauI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 815 TCCGGA T^CCGGA
AflII 1662 CTTAAG C^TTAAG
AocI 507 CCTGAGG CC^T[ACGT]AGG
BalI 564 TGGCCA TGG^CCA
BamHI 1423 GGATCC G^GATCC
BanI 1560 GGCACC G^G[CT][GA]CC
BclI 314 TGATCA T^GATCA
BcnI 405 CCGGG CC^[GC]GG
BfrI 1662 CTTAAG C^TTAAG
BglII 226 AGATCT A^GATCT
BlpI 757 GCTCAGC GC^T[ACGT]AGC
BpmI 451 CTCCAG CTGGAG(16/14)
Bpu1102I 757 GCTCAGC GC^T[ACGT]AGC
BsaWI 815 TCCGGA [AT]^CCGG[AT]
BseAI 815 TCCGGA T^CCGGA
Bsp1407I 1331 TGTACA T^GTACA
BspEI 815 TCCGGA T^CCGGA
BspHI 1370 TCATGA T^CATGA
BspMI 692 ACCTGC ACCTGC(4/8)
BsrGI 1331 TGTACA T^GTACA
BstXI 900 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 507 CCTGAGG CC^T[ACGT]AGG
CelII 757 GCTCAGC GC^T[ACGT]AGC
DraII 603 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1427 CCATGG C^C[GA][CT]GG
EaeI 564 TGGCCA [CT]^GGCC[GA]
EarI 1442 CTCTTC CTCTTC(1/4)
Eco81I 507 CCTGAGG CC^T[ACGT]AGG
EcoO109I 603 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1427 CCATGG C^C[AT][AT]GG
EcoT22I 777 ATGCAT ATGCA^T
FbaI 314 TGATCA T^GATCA
HgaI 1218 GCGTC GACGC(5/10)
HincII 1657 GTTGAC GT[CT]^[GA]AC
HindII 1657 GTTGAC GT[CT]^[GA]AC
HphI 1357 GGTGA GGTGA(8/7)
Ksp632I 1442 CTCTTC CTCTTC(1/4)
MaeII 1328 ACGT A^CGT
MaeIII 530 GTGAC ^GT[ACGT]AC
MluI 1121 ACGCGT A^CGCGT
MluNI 564 TGGCCA TGG^CCA
MroI 815 TCCGGA T^CCGGA
MscI 564 TGGCCA TGG^CCA
MspA1I 901 CAGCTG C[AC]G^C[GT]G
NciI 405 CCGGG CC^[GC]GG
NcoI 1427 CCATGG C^CATGG
NruI 1417 TCGCGA TCG^CGA
NsiI 777 ATGCAT ATGCA^T
PshAI 410 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 277 CTGCAG CTGCA^G
PvuII 901 CAGCTG CAG^CTG
RcaI 1370 TCATGA T^CATGA
SspBI 1331 TGTACA T^GTACA
StuI 480 AGGCCT AGG^CCT
StyI 1427 CCATGG C^C[AT][AT]GG
Tsp45I 530 GTGAC ^GT[GC]AC
XcmI 900 CCAGCTGTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1122 CGCG CG^CG
1418 CGCG
AseI 420 ATTAAT AT^TAAT
1588 ATTAAT
AsnI 420 ATTAAT AT^TAAT
1588 ATTAAT
Asp700I 648 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1305 GAAGCACTTC
AvaII 504 GGACC G^G[AT]CC
795 GGACC
BbsI 586 GAAGAC GAAGAC(2/6)
338 GTCTTC
BbvI 279 GCAGC GCAGC(8/12)
276 GCTGC
BpuAI 586 GAAGAC GAAGAC(2/6)
338 GTCTTC
BsaJI 405 CCGGGG C^C[ACGT][ACGT]GG
1427 CCATGG
BseRI 642 CTCCTC GAGGAG(10/8)
1386 CTCCTC
BsmI 168 GAATGC GAATGC(1/-1)
38 GCATTC
BspLU11I 1578 ACATGT A^CATGT
1647 ACATGT
BstUI 1122 CGCG CG^CG
1418 CGCG
Cac8I 34 GCCTGC GC[ACGT]^[ACGT]GC
857 GCAAGC
Fnu4HI 276 GCTGC GC^[ACGT]GC
279 GCAGC
HapII 405 CCGG C^CGG
816 CCGG
HpaII 405 CCGG C^CGG
816 CCGG
ItaI 276 GCTGC GC^[ACGT]GC
279 GCAGC
MspI 405 CCGG C^CGG
816 CCGG
MvnI 1122 CGCG CG^CG
1418 CGCG
MwoI 270 GCCATCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1516 GCAGAGAAAGC
NspI 1578 ACATGT [GA]CATG^[CT]
1647 ACATGT
PshBI 420 ATTAAT AT^TAAT
1588 ATTAAT
TseI 276 GCTGC G^C[AT]GC
279 GCAGC
XbaI 348 TCTAGA T^CTAGA
1152 TCTAGA
XmnI 648 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1305 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI BfaI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI CfoI Cfr13I DdeI DpnII
EcoRII FokI HaeIII HhaI HinP1I
HinfI MaeI MboI MboII MflI
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
XhoII