Restriction map for
pFN21AE6412
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbvCI BcnI BfrI
BlnI BlpI Bpu1102I BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI MfeI
MroI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 520 GGTACC G^GTACC
AccI 379 GTCTAC GT^[AC][GT]AC
AcsI 782 GAATTC [GA]^AATT[CT]
AcyI 298 GGCGTC G[GA]^CG[CT]C
Alw44I 1509 GTGCAC G^TGCAC
ApaLI 1509 GTGCAC G^TGCAC
ApoI 782 GAATTC [GA]^AATT[CT]
Asp718I 520 GGTACC G^GTACC
AspHI 1509 GTGCAC G[AT]GC[AT]^C
BalI 1376 TGGCCA TGG^CCA
BbrPI 1222 CACGTG CAC^GTG
BcgI 1738 GCAGGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 51 GTATCC GTATCC(6/5)
BclI 1295 TGATCA T^GATCA
BglI 290 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 360 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GGCGTC G[GA]^CG[CT]C
BsaI 12 GGTCTC GGTCTC(1/5)
BsaWI 1241 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1509 GTGCAC G[AT]GC[AT]^C
BsmBI 1275 CGTCTC CGTCTC(1/5)
BspLU11I 1211 ACATGT A^CATGT
BspMI 440 ACCTGC ACCTGC(4/8)
BsrFI 982 GCCGGC [GA]^CCGG[CT]
BstAPI 1026 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 971 CCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 982 GCCGGC [GA]^CCGG[CT]
EcoRI 782 GAATTC G^AATTC
FbaI 1295 TGATCA T^GATCA
Hin1I 298 GGCGTC G[GA]^CG[CT]C
KpnI 520 GGTACC GGTAC^C
KspI 1087 CCGCGG CCGC^GG
MamI 360 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 811 ACGCGT A^CGCGT
MluNI 1376 TGGCCA TGG^CCA
MscI 1376 TGGCCA TGG^CCA
MseI 745 TTAA T^TAA
NaeI 982 GCCGGC GCC^GGC
NgoMIV 982 GCCGGC G^CCGGC
PflMI 594 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1222 CACGTG CAC^GTG
PmlI 1222 CACGTG CAC^GTG
PvuII 1502 CAGCTG CAG^CTG
SacII 1087 CCGCGG CCGC^GG
SapI 1488 GCTCTTC GCTCTTC(1/4)
SmlI 1666 CTCAAG C^T[CT][GA]AG
TfiI 1688 GAATC G^A[AT]TC
Tru9I 745 TTAA T^TAA
Van91I 594 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 812 CGCG CG^CG
1088 CGCG
Aor51HI 1200 AGCGCT AGC^GCT
1421 AGCGCT
BamHI 590 GGATCC G^GATCC
822 GGATCC
BglII 317 AGATCT A^GATCT
578 AGATCT
BseRI 370 CTCCTC GAGGAG(10/8)
1115 CTCCTC
BsrBI 91 GAGCGG CCGCTC(-3/-3)
562 GAGCGG
BstUI 812 CGCG CG^CG
1088 CGCG
Eco47III 1200 AGCGCT AGC^GCT
1421 AGCGCT
HaeII 1200 AGCGCT [GA]GCGC^[CT]
1421 AGCGCT
HapII 983 CCGG C^CGG
1242 CCGG
HgaI 299 GCGTC GACGC(5/10)
813 GCGTC
HpaII 983 CCGG C^CGG
1242 CCGG
MslI 387 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATGCTGGTG
MspI 983 CCGG C^CGG
1242 CCGG
MvnI 812 CGCG CG^CG
1088 CGCG
NcoI 1462 CCATGG C^CATGG
1622 CCATGG
NspI 1211 ACATGT [GA]CATG^[CT]
1358 GCATGT
PpuMI 1740 AGGACCC [GA]G^G[AT]CC[CT]
1765 AGGACCT
SfcI 930 CTACAG C^T[GA][CT]AG
1650 CTACAG
Tsp509I 747 AATT ^AATT
783 AATT
XcmI 686 CCACAGGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1037 CCATTTCTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AvaII BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII